Restriction map for
pF1KA0806
(Linear) (Six-base) MAPSORT of: KIAA0806.seq Check: 9400 from: 1 to: 3195
KIAA0806.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
AatII G_ACGT'C
Cuts at: 0 1976 3195
Size: 1976 1219
Acc65I G'GTAC_C
Cuts at: 0 2689 3195
Size: 2689 506
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1066 3195
Size: 1066 2129
AflII C'TTAA_G
Cuts at: 0 720 760 1095 3195
Size: 720 40 335 2100
Fragments arranged by size:
2100 720 335 40
AleI CACnn'nnGTG
Cuts at: 0 1561 3195
Size: 1561 1634
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 141 196 3195
Size: 141 55 2999
Fragments arranged by size:
2999 141 55
AlwNI CAG_nnn'CTG
Cuts at: 0 39 2799 3195
Size: 39 2760 396
Fragments arranged by size:
2760 396 39
ApaLI G'TGCA_C
Cuts at: 0 1412 1554 3195
Size: 1412 142 1641
Fragments arranged by size:
1641 1412 142
ApoI r'AATT_y
Cuts at: 0 516 1020 1109 1638 1710 3195
Size: 516 504 89 529 72 1485
Fragments arranged by size:
1485 529 516 504 89 72
AvaI C'yCGr_G
Cuts at: 0 1937 1963 2123 2693 2744 3195
Size: 1937 26 160 570 51 451
Fragments arranged by size:
1937 570 451 160 51 26
AvrII C'CTAG_G
Cuts at: 0 15 3195
Size: 15 3180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2499 2532 2681 2714 3195
Size: 2499 33 149 33 481
Fragments arranged by size:
2499 481 149 33 33
BanI G'GyrC_C
Cuts at: 0 3 9 1846 2689 3195
Size: 3 6 1837 843 506
Fragments arranged by size:
1837 843 506 6 3
BanII G_rGCy'C
Cuts at: 0 2537 3195
Size: 2537 658
BbeI G_GCGC'C
Cuts at: 0 7 13 3195
Size: 7 6 3182
Fragments arranged by size:
3182 7 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 1968 2513 2561 3195
Size: 1968 545 48 634
Fragments arranged by size:
1968 634 545 48
BbvCI CC'TCA_GC
Cuts at: 0 451 2774 3195
Size: 451 2323 421
Fragments arranged by size:
2323 451 421
BciVI GTATCCnnnnn_n'
Cuts at: 0 1623 2850 3195
Size: 1623 1227 345
Fragments arranged by size:
1623 1227 345
BclI T'GATC_A
Cuts at: 0 2790 3195
Size: 2790 405
BfrBI ATG'CAT
Cuts at: 0 916 3195
Size: 916 2279
BglII A'GATC_T
Cuts at: 0 629 724 3195
Size: 629 95 2471
Fragments arranged by size:
2471 629 95
Bme1580I G_kGCm'C
Cuts at: 0 1416 1558 3193 3195
Size: 1416 142 1635 2
Fragments arranged by size:
1635 1416 142 2
BmgBI CAC'GTC
Cuts at: 0 1553 1626 1956 3195
Size: 1553 73 330 1239
Fragments arranged by size:
1553 1239 330 73
BmtI G_CTAG'C
Cuts at: 0 1432 3195
Size: 1432 1763
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 139 2103 3195
Size: 139 1964 1092
Fragments arranged by size:
1964 1092 139
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 403 457 1685 2125 2177 2721 3195
Size: 216 187 54 1228 440 52 544 474
Fragments arranged by size:
1228 544 474 440 216 187 54 52
Bpu10I CC'TnA_GC
Cuts at: 0 451 2774 3195
Size: 451 2323 421
Fragments arranged by size:
2323 451 421
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1255 1471 3195
Size: 1255 216 1724
Fragments arranged by size:
1724 1255 216
BsaI GGTCTCn'nnnn_
Cuts at: 0 127 2053 3195
Size: 127 1926 1142
Fragments arranged by size:
1926 1142 127
BsaHI Gr'CG_yC
Cuts at: 0 4 10 20 1973 3195
Size: 4 6 10 1953 1222
Fragments arranged by size:
1953 1222 10 6 4
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 401 431 2719 2749 3195
Size: 19 30 352 30 2288 30 446
Fragments arranged by size:
2288 446 352 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 85 2545 2791 3195
Size: 43 42 2460 246 404
Fragments arranged by size:
2460 404 246 43 42
BseYI C'CCAG_C
Cuts at: 0 39 2281 2557 3035 3195
Size: 39 2242 276 478 160
Fragments arranged by size:
2242 478 276 160 39
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1871 3195
Size: 1871 1324
BsiHKAI G_wGCw'C
Cuts at: 0 1416 1558 2537 3195
Size: 1416 142 979 658
Fragments arranged by size:
1416 979 658 142
BsmI GAATG_Cn'
Cuts at: 0 149 894 1213 3195
Size: 149 745 319 1982
Fragments arranged by size:
1982 745 319 149
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2182 3195
Size: 2182 1013
Bsp1286I G_dGCh'C
Cuts at: 0 1416 1558 2537 3193 3195
Size: 1416 142 979 656 2
Fragments arranged by size:
1416 979 656 142 2
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 109 2302 3195
Size: 109 2193 893
Fragments arranged by size:
2193 893 109
BsrBI CCG'CTC
Cuts at: 0 91 2966 3195
Size: 91 2875 229
Fragments arranged by size:
2875 229 91
BsrDI GCAATG_nn'
Cuts at: 0 77 2974 3195
Size: 77 2897 221
Fragments arranged by size:
2897 221 77
BsrFI r'CCGG_y
Cuts at: 0 6 3195
Size: 6 3189
BssSI C'ACGA_G
Cuts at: 0 827 3195
Size: 827 2368
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1959 2640 3195
Size: 1959 681 555
Fragments arranged by size:
1959 681 555
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2811 3195
Size: 2811 384
BstYI r'GATC_y
Cuts at: 0 629 724 1455 1827 3195
Size: 629 95 731 372 1368
Fragments arranged by size:
1368 731 629 372 95
BtgI C'CryG_G
Cuts at: 0 1850 3195
Size: 1850 1345
BtsI GCAGTG_nn'
Cuts at: 0 1556 1568 1578 3195
Size: 1556 12 10 1617
Fragments arranged by size:
1617 1556 12 10
DraI TTT'AAA
Cuts at: 0 1304 3195
Size: 1304 1891
DraIII CAC_nnn'GTG
Cuts at: 0 1595 3195
Size: 1595 1600
DrdI GACnn_nn'nnGTC
Cuts at: 0 1036 1157 3195
Size: 1036 121 2038
Fragments arranged by size:
2038 1036 121
EaeI y'GGCC_r
Cuts at: 0 1853 2328 3195
Size: 1853 475 867
Fragments arranged by size:
1853 867 475
EarI CTCTTCn'nnn_
Cuts at: 0 79 2376 3195
Size: 79 2297 819
Fragments arranged by size:
2297 819 79
EcoICRI GAG'CTC
Cuts at: 0 2535 3195
Size: 2535 660
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 216 403 457 1066 1685 2125 2177 2721
Size: 216 187 54 609 619 440 52 544
Cuts at: 2721 3195
Size: 474
Fragments arranged by size:
619 609 544 474 440 216 187 54
52
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3126 3195
Size: 3126 69
EcoO109I rG'GnC_Cy
Cuts at: 0 2741 3078 3195
Size: 2741 337 117
Fragments arranged by size:
2741 337 117
HaeII r_GCGC'y
Cuts at: 0 7 13 205 3195
Size: 7 6 192 2990
Fragments arranged by size:
2990 192 7 6
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 439 471 553 585 1789 1821 3195
Size: 439 32 82 32 1204 32 1374
Fragments arranged by size:
1374 1204 439 82 32 32 32
KasI G'GCGC_C
Cuts at: 0 3 9 3195
Size: 3 6 3186
Fragments arranged by size:
3186 6 3
KpnI G_GTAC'C
Cuts at: 0 2693 3195
Size: 2693 502
MfeI C'AATT_G
Cuts at: 0 2361 3195
Size: 2361 834
MscI TGG'CCA
Cuts at: 0 1855 2330 3195
Size: 1855 475 865
Fragments arranged by size:
1855 865 475
MslI CAynn'nnrTG
Cuts at: 0 1540 1561 2396 2434 2959 3071 3195
Size: 1540 21 835 38 525 112 124
Fragments arranged by size:
1540 835 525 124 112 38 21
MspA1I CmG'CkG
Cuts at: 0 741 966 2982 3195
Size: 741 225 2016 213
Fragments arranged by size:
2016 741 225 213
NaeI GCC'GGC
Cuts at: 0 8 3195
Size: 8 3187
NarI GG'CG_CC
Cuts at: 0 4 10 3195
Size: 4 6 3185
Fragments arranged by size:
3185 6 4
NcoI C'CATG_G
Cuts at: 0 1850 3195
Size: 1850 1345
NgoMIV G'CCGG_C
Cuts at: 0 6 3195
Size: 6 3189
NheI G'CTAG_C
Cuts at: 0 1428 3195
Size: 1428 1767
NsiI A_TGCA'T
Cuts at: 0 918 3195
Size: 918 2277
NspI r_CATG'y
Cuts at: 0 501 1953 3195
Size: 501 1452 1242
Fragments arranged by size:
1452 1242 501
PflMI CCAn_nnn'nTGG
Cuts at: 0 657 1595 3195
Size: 657 938 1600
Fragments arranged by size:
1600 938 657
PfoI T'CCnGG_A
Cuts at: 0 23 158 236 2737 3195
Size: 23 135 78 2501 458
Fragments arranged by size:
2501 458 135 78 23
PpuMI rG'GwC_Cy
Cuts at: 0 2741 3195
Size: 2741 454
PshAI GACnn'nnGTC
Cuts at: 0 167 3195
Size: 167 3028
PstI C_TGCA'G
Cuts at: 0 169 1563 1875 2238 2550 3195
Size: 169 1394 312 363 312 645
Fragments arranged by size:
1394 645 363 312 312 169
PvuII CAG'CTG
Cuts at: 0 966 3195
Size: 966 2229
SacI G_AGCT'C
Cuts at: 0 2537 3195
Size: 2537 658
SfcI C'TryA_G
Cuts at: 0 165 1559 1871 2016 2234 2511 2546 2604
Size: 165 1394 312 145 218 277 35 58
Cuts at: 2604 3195
Size: 591
Fragments arranged by size:
1394 591 312 277 218 165 145 58
35
SfoI GGC'GCC
Cuts at: 0 5 11 3195
Size: 5 6 3184
Fragments arranged by size:
3184 6 5
SgrAI Cr'CCGG_yG
Cuts at: 0 6 3195
Size: 6 3189
SmaI CCC'GGG
Cuts at: 0 2695 3195
Size: 2695 500
SmlI C'TyrA_G
Cuts at: 0 720 760 1095 1234 1486 1937 3195
Size: 720 40 335 139 252 451 1258
Fragments arranged by size:
1258 720 451 335 252 139 40
SpeI A'CTAG_T
Cuts at: 0 1684 3195
Size: 1684 1511
SphI G_CATG'C
Cuts at: 0 501 1953 3195
Size: 501 1452 1242
Fragments arranged by size:
1452 1242 501
SspI AAT'ATT
Cuts at: 0 363 3195
Size: 363 2832
StuI AGG'CCT
Cuts at: 0 3014 3195
Size: 3014 181
StyI C'CwwG_G
Cuts at: 0 15 542 705 1850 2313 2574 3195
Size: 15 527 163 1145 463 261 621
Fragments arranged by size:
1145 621 527 463 261 163 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1596 3195
Size: 1596 1599
TatI w'GTAC_w
Cuts at: 0 2841 3195
Size: 2841 354
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 401 433 2310 2342 3195
Size: 401 32 1877 32 853
Fragments arranged by size:
1877 853 401 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2799 3195
Size: 2799 396
XbaI T'CTAG_A
Cuts at: 0 52 1242 3195
Size: 52 1190 1953
Fragments arranged by size:
1953 1190 52
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2383 3195
Size: 2383 812
XhoI C'TCGA_G
Cuts at: 0 1937 3195
Size: 1937 1258
XmaI C'CCGG_G
Cuts at: 0 2693 3195
Size: 2693 502
XmnI GAAnn'nnTTC
Cuts at: 0 1213 1313 3195
Size: 1213 100 1882
Fragments arranged by size:
1882 1213 100
ZraI GAC'GTC
Cuts at: 0 1974 3195
Size: 1974 1221
Enzymes that do cut:
AatII Acc65I AcuI AflII AleI AlfI AlwNI ApaLI
ApoI AvaI AvrII BaeI BanI BanII BbeI BbsI
BbvCI BciVI BclI BfrBI BglII Bme1580I BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaHI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtsI
DraI DraIII DrdI EaeI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I KasI KpnI MfeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NheI NsiI NspI
PflMI PfoI PpuMI PshAI PstI PvuII SacI SfcI
SfoI SgrAI SmaI SmlI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflIII AgeI AhdI AloI
ApaI AscI AseI AsiSI BamHI BcgI BglI BlpI
BmrI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgZI ClaI
CspCI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI MluI MmeI NdeI NotI
NruI PacI PasI PciI PmeI PmlI PpiI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SgfI SnaBI SrfI
SwaI