Restriction map for
pF1KSDA0794
(Linear) (Six-base) MAPSORT of: KIAA0794.seq Check: 6243 from: 1 to: 1467
KIAA0794.orf
With 182 enzymes: SgfI *
October 24, 2006 15:14 ..
AclI AA'CG_TT
Cuts at: 0 1350 1467
Size: 1350 117
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 188 1467
Size: 58 130 1279
Fragments arranged by size:
1279 130 58
AgeI A'CCGG_T
Cuts at: 0 70 1467
Size: 70 1397
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 558 1467
Size: 558 909
BanI G'GyrC_C
Cuts at: 0 725 1467
Size: 725 742
BanII G_rGCy'C
Cuts at: 0 19 176 1467
Size: 19 157 1291
Fragments arranged by size:
1291 157 19
BbsI GAAGACnn'nnnn_
Cuts at: 0 863 1467
Size: 863 604
BciVI GTATCCnnnnn_n'
Cuts at: 0 1265 1330 1467
Size: 1265 65 137
Fragments arranged by size:
1265 137 65
BfrBI ATG'CAT
Cuts at: 0 447 1467
Size: 447 1020
BglII A'GATC_T
Cuts at: 0 417 1467
Size: 417 1050
Bme1580I G_kGCm'C
Cuts at: 0 237 828 1467
Size: 237 591 639
Fragments arranged by size:
639 591 237
BmrI ACTGGGnnnn_n'
Cuts at: 0 170 1467
Size: 170 1297
BmtI G_CTAG'C
Cuts at: 0 1309 1467
Size: 1309 158
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1195 1467
Size: 1195 272
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1421 1467
Size: 1421 46
BsaAI yAC'GTr
Cuts at: 0 1323 1467
Size: 1323 144
BsaHI Gr'CG_yC
Cuts at: 0 25 1467
Size: 25 1442
BsaWI w'CCGG_w
Cuts at: 0 70 1467
Size: 70 1397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1 31 1467
Size: 1 30 1436
Fragments arranged by size:
1436 30 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 398 994 1093 1467
Size: 398 596 99 374
Fragments arranged by size:
596 398 374 99
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 293 1345 1467
Size: 293 1052 122
Fragments arranged by size:
1052 293 122
BsiHKAI G_wGCw'C
Cuts at: 0 291 1467
Size: 291 1176
Bsp1286I G_dGCh'C
Cuts at: 0 19 176 237 291 828 1467
Size: 19 157 61 54 537 639
Fragments arranged by size:
639 537 157 61 54 19
BspHI T'CATG_A
Cuts at: 0 621 1467
Size: 621 846
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 603 1467
Size: 603 864
BsrBI CCG'CTC
Cuts at: 0 938 1467
Size: 938 529
BsrDI GCAATG_nn'
Cuts at: 0 1411 1467
Size: 1411 56
BsrFI r'CCGG_y
Cuts at: 0 70 1467
Size: 70 1397
BsrGI T'GTAC_A
Cuts at: 0 1452 1467
Size: 1452 15
BstYI r'GATC_y
Cuts at: 0 417 1467
Size: 417 1050
BtgI C'CryG_G
Cuts at: 0 9 20 697 1467
Size: 9 11 677 770
Fragments arranged by size:
770 677 11 9
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 569 1467
Size: 569 898
BtsI GCAGTG_nn'
Cuts at: 0 981 1467
Size: 981 486
ClaI AT'CG_AT
Cuts at: 0 392 1467
Size: 392 1075
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 459 478 494 513 1467
Size: 459 19 16 19 954
Fragments arranged by size:
954 459 19 19 16
EaeI y'GGCC_r
Cuts at: 0 427 480 1467
Size: 427 53 987
Fragments arranged by size:
987 427 53
EarI CTCTTCn'nnn_
Cuts at: 0 164 1010 1289 1467
Size: 164 846 279 178
Fragments arranged by size:
846 279 178 164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 188 1195 1467
Size: 58 130 1007 272
Fragments arranged by size:
1007 272 130 58
HaeII r_GCGC'y
Cuts at: 0 37 1467
Size: 37 1430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 374 406 922 934 954 966 973 1005
Size: 374 32 516 12 20 12 7 32
Cuts at: 1005 1467
Size: 462
Fragments arranged by size:
516 462 374 32 32 20 12 12
7
MscI TGG'CCA
Cuts at: 0 482 1467
Size: 482 985
MslI CAynn'nnrTG
Cuts at: 0 540 626 1467
Size: 540 86 841
Fragments arranged by size:
841 540 86
MspA1I CmG'CkG
Cuts at: 0 22 1095 1156 1242 1467
Size: 22 1073 61 86 225
Fragments arranged by size:
1073 225 86 61 22
NdeI CA'TA_TG
Cuts at: 0 98 1467
Size: 98 1369
NheI G'CTAG_C
Cuts at: 0 1305 1467
Size: 1305 162
NsiI A_TGCA'T
Cuts at: 0 449 1467
Size: 449 1018
NspI r_CATG'y
Cuts at: 0 539 1467
Size: 539 928
PflMI CCAn_nnn'nTGG
Cuts at: 0 283 1467
Size: 283 1184
PfoI T'CCnGG_A
Cuts at: 0 332 590 1122 1467
Size: 332 258 532 345
Fragments arranged by size:
532 345 332 258
PmlI CAC'GTG
Cuts at: 0 1323 1467
Size: 1323 144
PsiI TTA'TAA
Cuts at: 0 659 1467
Size: 659 808
PvuII CAG'CTG
Cuts at: 0 1156 1242 1467
Size: 1156 86 225
Fragments arranged by size:
1156 225 86
SacII CC_GC'GG
Cuts at: 0 23 1467
Size: 23 1444
SapI GCTCTTCn'nnn_
Cuts at: 0 164 1467
Size: 164 1303
SmlI C'TyrA_G
Cuts at: 0 1436 1467
Size: 1436 31
SspI AAT'ATT
Cuts at: 0 585 1467
Size: 585 882
StuI AGG'CCT
Cuts at: 0 1427 1467
Size: 1427 40
StyI C'CwwG_G
Cuts at: 0 1143 1467
Size: 1143 324
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 447 763 1467
Size: 447 316 704
Fragments arranged by size:
704 447 316
TatI w'GTAC_w
Cuts at: 0 1452 1467
Size: 1452 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1 33 1356 1388 1467
Size: 1 32 1323 32 79
Fragments arranged by size:
1323 79 32 32 1
XmnI GAAnn'nnTTC
Cuts at: 0 227 353 1467
Size: 227 126 1114
Fragments arranged by size:
1114 227 126
Enzymes that do cut:
AclI AcuI AgeI AlfI BanI BanII BbsI BciVI
BfrBI BglII Bme1580I BmrI BmtI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BsgI BsiHKAI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BstYI BtgI BtgZI
BtsI ClaI CspCI EaeI EarI Eco57MI HaeII Hin4I
MscI MslI MspA1I NdeI NheI NsiI NspI PflMI
PfoI PmlI PsiI PvuII SacII SapI SmlI SspI
StuI StyI TaqII TatI TstI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AhdI
AleI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BbeI BbvCI BcgI
BclI BglI BlpI BmgBI BplI Bpu10I BsaI BsaBI
BseYI BsiEI BsiWI BsmI BsmBI BspEI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NcoI NgoMIV
NotI NruI PacI PasI PciI PmeI PpiI PpuMI
PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SalI SanDI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SwaI
Tth111I XbaI XcmI XhoI XmaI ZraI