Restriction map for
pF1KSDA0759
(Linear) (Six-base) MAPSORT of: KIAA0759.seq Check: 6423 from: 1 to: 2010
KIAA0759.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AatII G_ACGT'C
Cuts at: 0 1726 2010
Size: 1726 284
Acc65I G'GTAC_C
Cuts at: 0 1696 2010
Size: 1696 314
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 724 1931 1943 2010
Size: 296 428 1207 12 67
Fragments arranged by size:
1207 428 296 67 12
AflIII A'CryG_T
Cuts at: 0 76 2010
Size: 76 1934
AhdI GACnn_n'nnGTC
Cuts at: 0 324 2010
Size: 324 1686
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 94 126 2010
Size: 94 32 1884
Fragments arranged by size:
1884 94 32
AlwNI CAG_nnn'CTG
Cuts at: 0 702 768 1274 2010
Size: 702 66 506 736
Fragments arranged by size:
736 702 506 66
ApaI G_GGCC'C
Cuts at: 0 154 239 2010
Size: 154 85 1771
Fragments arranged by size:
1771 154 85
ApoI r'AATT_y
Cuts at: 0 650 853 1302 2010
Size: 650 203 449 708
Fragments arranged by size:
708 650 449 203
AvaI C'yCGr_G
Cuts at: 0 146 2010
Size: 146 1864
BanI G'GyrC_C
Cuts at: 0 499 1696 2010
Size: 499 1197 314
Fragments arranged by size:
1197 499 314
BanII G_rGCy'C
Cuts at: 0 154 239 433 467 784 791 1352 2010
Size: 154 85 194 34 317 7 561 658
Fragments arranged by size:
658 561 317 194 154 85 34 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 1400 2010
Size: 1400 610
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 976 1010 2010
Size: 976 34 1000
Fragments arranged by size:
1000 976 34
BclI T'GATC_A
Cuts at: 0 1851 2010
Size: 1851 159
BfrBI ATG'CAT
Cuts at: 0 1660 2010
Size: 1660 350
BglI GCCn_nnn'nGGC
Cuts at: 0 1219 2010
Size: 1219 791
Bme1580I G_kGCm'C
Cuts at: 0 154 239 504 572 2010
Size: 154 85 265 68 1438
Fragments arranged by size:
1438 265 154 85 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 462 487 873 921 983 2010
Size: 462 25 386 48 62 1027
Fragments arranged by size:
1027 462 386 62 48 25
BmtI G_CTAG'C
Cuts at: 0 1040 1981 2010
Size: 1040 941 29
Fragments arranged by size:
1040 941 29
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 693 2010
Size: 693 1317
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 314 430 567 871 1336 1668 2010
Size: 314 116 137 304 465 332 342
Fragments arranged by size:
465 342 332 314 304 137 116
Bpu10I CC'TnA_GC
Cuts at: 0 155 560 695 761 2010
Size: 155 405 135 66 1249
Fragments arranged by size:
1249 405 155 135 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1669 1690 1864 2010
Size: 1669 21 174 146
Fragments arranged by size:
1669 174 146 21
BsaHI Gr'CG_yC
Cuts at: 0 1723 2010
Size: 1723 287
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 454 484 2010
Size: 454 30 1526
Fragments arranged by size:
1526 454 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 981 2010
Size: 412 569 1029
Fragments arranged by size:
1029 569 412
BseYI C'CCAG_C
Cuts at: 0 531 683 1444 1748 2010
Size: 531 152 761 304 262
Fragments arranged by size:
761 531 304 262 152
BsiHKAI G_wGCw'C
Cuts at: 0 218 784 791 1352 2010
Size: 218 566 7 561 658
Fragments arranged by size:
658 566 561 218 7
BsmI GAATG_Cn'
Cuts at: 0 309 2010
Size: 309 1701
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1503 1900 2010
Size: 1503 397 110
Fragments arranged by size:
1503 397 110
Bsp1286I G_dGCh'C
Cuts at: 0 154 218 239 433 467 504 572 784
Size: 154 64 21 194 34 37 68 212
Cuts at: 784 791 1352 2010
Size: 7 561 658
Fragments arranged by size:
658 561 212 194 154 68 64 37
34 21 7
BspHI T'CATG_A
Cuts at: 0 645 2010
Size: 645 1365
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 32 1632 2010
Size: 32 1600 378
Fragments arranged by size:
1600 378 32
BsrDI GCAATG_nn'
Cuts at: 0 798 2010
Size: 798 1212
BsrFI r'CCGG_y
Cuts at: 0 33 2010
Size: 33 1977
BstAPI GCAn_nnn'nTGC
Cuts at: 0 481 1274 2010
Size: 481 793 736
Fragments arranged by size:
793 736 481
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1230 1360 1452 2010
Size: 1230 130 92 558
Fragments arranged by size:
1230 558 130 92
BtgI C'CryG_G
Cuts at: 0 137 1359 2010
Size: 137 1222 651
Fragments arranged by size:
1222 651 137
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1214 1542 2010
Size: 1214 328 468
Fragments arranged by size:
1214 468 328
BtsI GCAGTG_nn'
Cuts at: 0 193 403 523 635 1272 1707 2010
Size: 193 210 120 112 637 435 303
Fragments arranged by size:
637 435 303 210 193 120 112
EaeI y'GGCC_r
Cuts at: 0 35 506 1220 1250 1521 1755 2010
Size: 35 471 714 30 271 234 255
Fragments arranged by size:
714 471 271 255 234 35 30
EarI CTCTTCn'nnn_
Cuts at: 0 52 293 518 572 1907 1962 2010
Size: 52 241 225 54 1335 55 48
Fragments arranged by size:
1335 241 225 55 54 52 48
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1012 1249 2010
Size: 1012 237 761
Fragments arranged by size:
1012 761 237
EcoICRI GAG'CTC
Cuts at: 0 782 789 1350 2010
Size: 782 7 561 660
Fragments arranged by size:
782 660 561 7
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 296 314 430 567 724 871 1336 1668
Size: 296 18 116 137 157 147 465 332
Cuts at: 1668 1931 1943 2010
Size: 263 12 67
Fragments arranged by size:
465 332 296 263 157 147 137 116
67 18 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 760 847 1683 2010
Size: 760 87 836 327
Fragments arranged by size:
836 760 327 87
EcoO109I rG'GnC_Cy
Cuts at: 0 150 151 174 235 236 528 603 699
Size: 150 1 23 61 1 292 75 96
Cuts at: 699 765 867 1296 1431 2010
Size: 66 102 429 135 579
Fragments arranged by size:
579 429 292 150 135 102 96 75
66 61 23 1 1
EcoRI G'AATT_C
Cuts at: 0 853 2010
Size: 853 1157
EcoRV GAT'ATC
Cuts at: 0 876 2010
Size: 876 1134
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 273 305 310 342 2010
Size: 273 32 5 32 1668
Fragments arranged by size:
1668 273 32 32 5
KpnI G_GTAC'C
Cuts at: 0 1700 2010
Size: 1700 310
MfeI C'AATT_G
Cuts at: 0 819 2010
Size: 819 1191
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 440 2010
Size: 440 1570
MscI TGG'CCA
Cuts at: 0 508 1222 1252 2010
Size: 508 714 30 758
Fragments arranged by size:
758 714 508 30
MslI CAynn'nnrTG
Cuts at: 0 739 1774 2010
Size: 739 1035 236
Fragments arranged by size:
1035 739 236
MspA1I CmG'CkG
Cuts at: 0 339 535 921 1155 1822 2010
Size: 339 196 386 234 667 188
Fragments arranged by size:
667 386 339 234 196 188
NaeI GCC'GGC
Cuts at: 0 35 2010
Size: 35 1975
NcoI C'CATG_G
Cuts at: 0 137 1359 2010
Size: 137 1222 651
Fragments arranged by size:
1222 651 137
NgoMIV G'CCGG_C
Cuts at: 0 33 2010
Size: 33 1977
NheI G'CTAG_C
Cuts at: 0 1036 1977 2010
Size: 1036 941 33
Fragments arranged by size:
1036 941 33
NsiI A_TGCA'T
Cuts at: 0 1662 2010
Size: 1662 348
NspI r_CATG'y
Cuts at: 0 80 2010
Size: 80 1930
PasI CC'CwG_GG
Cuts at: 0 1452 2010
Size: 1452 558
PciI A'CATG_T
Cuts at: 0 76 2010
Size: 76 1934
PfoI T'CCnGG_A
Cuts at: 0 291 887 896 1684 2010
Size: 291 596 9 788 326
Fragments arranged by size:
788 596 326 291 9
PpuMI rG'GwC_Cy
Cuts at: 0 528 765 867 1296 2010
Size: 528 237 102 429 714
Fragments arranged by size:
714 528 429 237 102
PsiI TTA'TAA
Cuts at: 0 749 2010
Size: 749 1261
PspOMI G'GGCC_C
Cuts at: 0 150 235 2010
Size: 150 85 1775
Fragments arranged by size:
1775 150 85
PstI C_TGCA'G
Cuts at: 0 185 360 1430 1555 2010
Size: 185 175 1070 125 455
Fragments arranged by size:
1070 455 185 175 125
PvuII CAG'CTG
Cuts at: 0 339 921 1822 2010
Size: 339 582 901 188
Fragments arranged by size:
901 582 339 188
SacI G_AGCT'C
Cuts at: 0 784 791 1352 2010
Size: 784 7 561 658
Fragments arranged by size:
784 658 561 7
SanDI GG'GwC_CC
Cuts at: 0 867 2010
Size: 867 1143
SapI GCTCTTCn'nnn_
Cuts at: 0 1962 2010
Size: 1962 48
ScaI AGT'ACT
Cuts at: 0 1051 2010
Size: 1051 959
SfcI C'TryA_G
Cuts at: 0 181 356 1426 1551 1691 2010
Size: 181 175 1070 125 140 319
Fragments arranged by size:
1070 319 181 175 140 125
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1219 2010
Size: 1219 791
SmlI C'TyrA_G
Cuts at: 0 1648 1669 1879 2010
Size: 1648 21 210 131
Fragments arranged by size:
1648 210 131 21
StuI AGG'CCT
Cuts at: 0 1130 2010
Size: 1130 880
StyI C'CwwG_G
Cuts at: 0 137 1359 2010
Size: 137 1222 651
Fragments arranged by size:
1222 651 137
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 487 697 965 2010
Size: 487 210 268 1045
Fragments arranged by size:
1045 487 268 210
TatI w'GTAC_w
Cuts at: 0 1049 2010
Size: 1049 961
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1989 2010
Size: 1989 21
ZraI GAC'GTC
Cuts at: 0 1724 2010
Size: 1724 286
Enzymes that do cut:
AatII Acc65I AcuI AflIII AhdI AloI AlwNI ApaI
ApoI AvaI BanI BanII BbsI BcgI BclI BfrBI
BglI Bme1580I BmrI BmtI BplI BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BstAPI BstXI BtgI BtgZI
BtsI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV Hin4I KpnI MfeI MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV NheI NsiI NspI PasI
PciI PfoI PpuMI PsiI PspOMI PstI PvuII SacI
SanDI SapI ScaI SfcI SfiI SmlI StuI StyI
TaqII TatI XcmI ZraI
Enzymes that do not cut:
AarI AccI AclI AfeI AflII AgeI AleI AlfI
ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI
BbvCI BciVI BglII BlpI BmgBI BsaI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BspEI BsrBI BsrGI BssHII
BssSI BstBI BstEII BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EagI FalI FseI FspI FspAI
HaeII HincII HindIII HpaI KasI MluI NarI NdeI
NotI NruI PacI PflMI PmeI PmlI PpiI PshAI
PspXI PsrI PvuI RsrII SacII SalI SbfI SexAI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI XmnI