Restriction map for
pF1KSDA0669
(Linear) (Six-base) MAPSORT of: KIAA0669.seq Check: 7932 from: 1 to: 2340
KIAA0669.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AatII G_ACGT'C
Cuts at: 0 131 152 2340
Size: 131 21 2188
Fragments arranged by size:
2188 131 21
AccI GT'mk_AC
Cuts at: 0 1564 1713 1772 2340
Size: 1564 149 59 568
Fragments arranged by size:
1564 568 149 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1867 2340
Size: 1867 473
AflIII A'CryG_T
Cuts at: 0 469 541 1997 2340
Size: 469 72 1456 343
Fragments arranged by size:
1456 469 343 72
AlwNI CAG_nnn'CTG
Cuts at: 0 442 969 2340
Size: 442 527 1371
Fragments arranged by size:
1371 527 442
ApaI G_GGCC'C
Cuts at: 0 713 956 1030 2340
Size: 713 243 74 1310
Fragments arranged by size:
1310 713 243 74
AvaI C'yCGr_G
Cuts at: 0 155 241 712 885 955 2340
Size: 155 86 471 173 70 1385
Fragments arranged by size:
1385 471 173 155 86 70
BanI G'GyrC_C
Cuts at: 0 655 1039 1198 1240 1506 2340
Size: 655 384 159 42 266 834
Fragments arranged by size:
834 655 384 266 159 42
BanII G_rGCy'C
Cuts at: 0 424 521 713 956 1030 1213 2340
Size: 424 97 192 243 74 183 1127
Fragments arranged by size:
1127 424 243 192 183 97 74
BbeI G_GCGC'C
Cuts at: 0 659 1043 1202 2340
Size: 659 384 159 1138
Fragments arranged by size:
1138 659 384 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 1280 1958 2340
Size: 1280 678 382
Fragments arranged by size:
1280 678 382
BbvCI CC'TCA_GC
Cuts at: 0 1079 2334 2340
Size: 1079 1255 6
Fragments arranged by size:
1255 1079 6
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1350 1384 2340
Size: 1350 34 956
Fragments arranged by size:
1350 956 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1085 2340
Size: 1085 1255
BclI T'GATC_A
Cuts at: 0 491 2217 2340
Size: 491 1726 123
Fragments arranged by size:
1726 491 123
BglI GCCn_nnn'nGGC
Cuts at: 0 64 379 1048 1222 2340
Size: 64 315 669 174 1118
Fragments arranged by size:
1118 669 315 174 64
BlpI GC'TnA_GC
Cuts at: 0 1621 2340
Size: 1621 719
Bme1580I G_kGCm'C
Cuts at: 0 713 956 1030 1243 1373 1511 1541 2340
Size: 713 243 74 213 130 138 30 799
Fragments arranged by size:
799 713 243 213 138 130 74 30
BmgBI CAC'GTC
Cuts at: 0 542 2340
Size: 542 1798
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 1602 2340
Size: 1428 174 738
Fragments arranged by size:
1428 738 174
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 213 2340
Size: 147 66 2127
Fragments arranged by size:
2127 147 66
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 2340
Size: 1385 955
Bpu10I CC'TnA_GC
Cuts at: 0 1079 1680 1729 2334 2340
Size: 1079 601 49 605 6
Fragments arranged by size:
1079 605 601 49 6
BsaAI yAC'GTr
Cuts at: 0 1152 2340
Size: 1152 1188
BsaHI Gr'CG_yC
Cuts at: 0 128 149 656 1040 1199 2340
Size: 128 21 507 384 159 1141
Fragments arranged by size:
1141 507 384 159 128 21
BsaWI w'CCGG_w
Cuts at: 0 661 2340
Size: 661 1679
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 118 148 302 305 332 335 656 686
Size: 118 30 154 3 27 3 321 30
Cuts at: 686 1379 1409 2033 2063 2295 2325 2340
Size: 693 30 624 30 232 30 15
Fragments arranged by size:
693 624 321 232 154 118 30 30
30 30 30 27 15 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 123 193 253 326 329 2322 2340
Size: 123 70 60 73 3 1993 18
Fragments arranged by size:
1993 123 73 70 60 18 3
BseYI C'CCAG_C
Cuts at: 0 410 791 931 1061 1292 1297 1423 1751
Size: 410 381 140 130 231 5 126 328
Cuts at: 1751 2340
Size: 589
Fragments arranged by size:
589 410 381 328 231 140 130 126
5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1417 2340
Size: 1417 923
BsiEI CG_ry'CG
Cuts at: 0 534 899 2340
Size: 534 365 1441
Fragments arranged by size:
1441 534 365
BsiHKAI G_wGCw'C
Cuts at: 0 399 2340
Size: 399 1941
BsmI GAATG_Cn'
Cuts at: 0 1257 2340
Size: 1257 1083
BsmBI CGTCTCn'nnnn_
Cuts at: 0 135 156 202 255 2340
Size: 135 21 46 53 2085
Fragments arranged by size:
2085 135 53 46 21
Bsp1286I G_dGCh'C
Cuts at: 0 399 424 521 713 956 1030 1213 1243
Size: 399 25 97 192 243 74 183 30
Cuts at: 1243 1373 1511 1541 2340
Size: 130 138 30 799
Fragments arranged by size:
799 399 243 192 183 138 130 97
74 30 30 25
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1148 1332 2340
Size: 1148 184 1008
Fragments arranged by size:
1148 1008 184
BsrBI CCG'CTC
Cuts at: 0 512 639 949 1543 2340
Size: 512 127 310 594 797
Fragments arranged by size:
797 594 512 310 127
BsrDI GCAATG_nn'
Cuts at: 0 1823 2084 2220 2340
Size: 1823 261 136 120
Fragments arranged by size:
1823 261 136 120
BsrFI r'CCGG_y
Cuts at: 0 12 999 1156 1195 1243 2340
Size: 12 987 157 39 48 1097
Fragments arranged by size:
1097 987 157 48 39 12
BssHII G'CGCG_C
Cuts at: 0 1268 2340
Size: 1268 1072
BstXI CCAn_nnnn'nTGG
Cuts at: 0 360 1669 2340
Size: 360 1309 671
Fragments arranged by size:
1309 671 360
BstYI r'GATC_y
Cuts at: 0 589 2082 2340
Size: 589 1493 258
Fragments arranged by size:
1493 589 258
Bsu36I CC'TnA_GG
Cuts at: 0 176 2340
Size: 176 2164
BtgI C'CryG_G
Cuts at: 0 53 161 504 1046 1217 1235 1307 1502
Size: 53 108 343 542 171 18 72 195
Cuts at: 1502 2340
Size: 838
Fragments arranged by size:
838 542 343 195 171 108 72 53
18
BtsI GCAGTG_nn'
Cuts at: 0 1040 1470 2285 2340
Size: 1040 430 815 55
Fragments arranged by size:
1040 815 430 55
DraI TTT'AAA
Cuts at: 0 2135 2340
Size: 2135 205
DraIII CAC_nnn'GTG
Cuts at: 0 1503 2340
Size: 1503 837
DrdI GACnn_nn'nnGTC
Cuts at: 0 577 744 2340
Size: 577 167 1596
Fragments arranged by size:
1596 577 167
EaeI y'GGCC_r
Cuts at: 0 14 65 531 1125 1154 1223 1245 1907
Size: 14 51 466 594 29 69 22 662
Cuts at: 1907 2340
Size: 433
Fragments arranged by size:
662 594 466 433 69 51 29 22
14
EagI C'GGCC_G
Cuts at: 0 531 2340
Size: 531 1809
EarI CTCTTCn'nnn_
Cuts at: 0 19 200 204 2340
Size: 19 181 4 2136
Fragments arranged by size:
2136 181 19 4
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 417 1436 2340
Size: 417 1019 904
Fragments arranged by size:
1019 904 417
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1385 1867 2340
Size: 1385 482 473
Fragments arranged by size:
1385 482 473
EcoO109I rG'GnC_Cy
Cuts at: 0 952 1027 2340
Size: 952 75 1313
Fragments arranged by size:
1313 952 75
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 48 229 2186 2340
Size: 48 181 1957 154
Fragments arranged by size:
1957 181 154 48
FseI GG_CCGG'CC
Cuts at: 0 1160 2340
Size: 1160 1180
HaeII r_GCGC'y
Cuts at: 0 349 659 1043 1202 1687 2340
Size: 349 310 384 159 485 653
Fragments arranged by size:
653 485 384 349 310 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 118 150 302 334 2017 2049 2209 2241
Size: 118 32 152 32 1683 32 160 32
Cuts at: 2241 2340
Size: 99
Fragments arranged by size:
1683 160 152 118 99 32 32 32
32
HincII GTy'rAC
Cuts at: 0 1773 1889 2340
Size: 1773 116 451
Fragments arranged by size:
1773 451 116
HpaI GTT'AAC
Cuts at: 0 1889 2340
Size: 1889 451
KasI G'GCGC_C
Cuts at: 0 655 1039 1198 2340
Size: 655 384 159 1142
Fragments arranged by size:
1142 655 384 159
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 743 2340
Size: 743 1597
MscI TGG'CCA
Cuts at: 0 67 1909 2340
Size: 67 1842 431
Fragments arranged by size:
1842 431 67
MspA1I CmG'CkG
Cuts at: 0 279 286 647 873 1048 1473 1620 2340
Size: 279 7 361 226 175 425 147 720
Fragments arranged by size:
720 425 361 279 226 175 147 7
NaeI GCC'GGC
Cuts at: 0 14 1001 1158 2340
Size: 14 987 157 1182
Fragments arranged by size:
1182 987 157 14
NarI GG'CG_CC
Cuts at: 0 656 1040 1199 2340
Size: 656 384 159 1141
Fragments arranged by size:
1141 656 384 159
NcoI C'CATG_G
Cuts at: 0 1307 2340
Size: 1307 1033
NgoMIV G'CCGG_C
Cuts at: 0 12 999 1156 2340
Size: 12 987 157 1184
Fragments arranged by size:
1184 987 157 12
NspI r_CATG'y
Cuts at: 0 473 1669 2340
Size: 473 1196 671
Fragments arranged by size:
1196 671 473
PasI CC'CwG_GG
Cuts at: 0 942 2340
Size: 942 1398
PciI A'CATG_T
Cuts at: 0 469 2340
Size: 469 1871
PmlI CAC'GTG
Cuts at: 0 1152 2340
Size: 1152 1188
PspOMI G'GGCC_C
Cuts at: 0 709 952 1026 2340
Size: 709 243 74 1314
Fragments arranged by size:
1314 709 243 74
PstI C_TGCA'G
Cuts at: 0 447 1035 1145 1166 1533 2340
Size: 447 588 110 21 367 807
Fragments arranged by size:
807 588 447 367 110 21
PvuII CAG'CTG
Cuts at: 0 279 1620 2340
Size: 279 1341 720
Fragments arranged by size:
1341 720 279
SacII CC_GC'GG
Cuts at: 0 1049 2340
Size: 1049 1291
SalI G'TCGA_C
Cuts at: 0 1771 2340
Size: 1771 569
SapI GCTCTTCn'nnn_
Cuts at: 0 19 204 2340
Size: 19 185 2136
Fragments arranged by size:
2136 185 19
ScaI AGT'ACT
Cuts at: 0 618 2340
Size: 618 1722
SexAI A'CCwGG_T
Cuts at: 0 1485 2340
Size: 1485 855
SfcI C'TryA_G
Cuts at: 0 443 522 1031 1141 1162 1529 2340
Size: 443 79 509 110 21 367 811
Fragments arranged by size:
811 509 443 367 110 79 21
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 64 2340
Size: 64 2276
SfoI GGC'GCC
Cuts at: 0 657 1041 1200 2340
Size: 657 384 159 1140
Fragments arranged by size:
1140 657 384 159
SgrAI Cr'CCGG_yG
Cuts at: 0 1195 2340
Size: 1195 1145
SmaI CCC'GGG
Cuts at: 0 243 887 957 2340
Size: 243 644 70 1383
Fragments arranged by size:
1383 644 243 70
StyI C'CwwG_G
Cuts at: 0 1307 2340
Size: 1307 1033
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1399 1447 2340
Size: 1399 48 893
Fragments arranged by size:
1399 893 48
TatI w'GTAC_w
Cuts at: 0 616 2340
Size: 616 1724
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 656 688 1379 1411 2340
Size: 656 32 691 32 929
Fragments arranged by size:
929 691 656 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 60 1233 1305 2340
Size: 60 1173 72 1035
Fragments arranged by size:
1173 1035 72 60
XmaI C'CCGG_G
Cuts at: 0 241 885 955 2340
Size: 241 644 70 1385
Fragments arranged by size:
1385 644 241 70
ZraI GAC'GTC
Cuts at: 0 129 150 2340
Size: 129 21 2190
Fragments arranged by size:
2190 129 21
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI ApaI AvaI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BssHII
BstXI BstYI Bsu36I BtgI BtsI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I FalI FseI
HaeII Hin4I HincII HpaI KasI MmeI MscI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PciI PmlI
PspOMI PstI PvuII SacII SalI SapI ScaI SexAI
SfcI SfiI SfoI SgrAI SmaI StyI TaqII TatI
TstI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BfrBI BglII BmtI BpuEI BsaI BsaBI
BsiWI BspEI BspHI BsrGI BssSI BstAPI BstBI BstEII
BstZ17I BtgZI ClaI CspCI EcoICRI EcoNI EcoRI EcoRV
FspI FspAI HindIII KpnI MfeI MluI MslI NdeI
NheI NotI NruI NsiI PacI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SanDI SbfI SgfI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI