Restriction map for
pF1KSDA0668
(Linear) (Six-base) MAPSORT of: KIAA0668.seq Check: 1489 from: 1 to: 2151
KIAA0668.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1569 2119 2151
Size: 1569 550 32
Fragments arranged by size:
1569 550 32
AatII G_ACGT'C
Cuts at: 0 599 2151
Size: 599 1552
Acc65I G'GTAC_C
Cuts at: 0 563 1745 2151
Size: 563 1182 406
Fragments arranged by size:
1182 563 406
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 513 649 1048 1192 1309 1411 2109 2151
Size: 513 136 399 144 117 102 698 42
Fragments arranged by size:
698 513 399 144 136 117 102 42
AfeI AGC'GCT
Cuts at: 0 1612 2151
Size: 1612 539
AleI CACnn'nnGTG
Cuts at: 0 1483 2151
Size: 1483 668
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1351 1383 2151
Size: 1351 32 768
Fragments arranged by size:
1351 768 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1170 1272 1605 2151
Size: 1170 102 333 546
Fragments arranged by size:
1170 546 333 102
ApaI G_GGCC'C
Cuts at: 0 181 2151
Size: 181 1970
ApaLI G'TGCA_C
Cuts at: 0 1582 1786 2151
Size: 1582 204 365
Fragments arranged by size:
1582 365 204
ApoI r'AATT_y
Cuts at: 0 877 2151
Size: 877 1274
AvaI C'yCGr_G
Cuts at: 0 393 833 1397 1763 2101 2151
Size: 393 440 564 366 338 50
Fragments arranged by size:
564 440 393 366 338 50
BamHI G'GATC_C
Cuts at: 0 2078 2151
Size: 2078 73
BanI G'GyrC_C
Cuts at: 0 553 563 1745 2151
Size: 553 10 1182 406
Fragments arranged by size:
1182 553 406 10
BanII G_rGCy'C
Cuts at: 0 76 181 253 388 490 942 1219 1300
Size: 76 105 72 135 102 452 277 81
Cuts at: 1300 1424 2144 2151
Size: 124 720 7
Fragments arranged by size:
720 452 277 135 124 105 102 81
76 72 7
BbsI GAAGACnn'nnnn_
Cuts at: 0 591 637 1118 1964 2151
Size: 591 46 481 846 187
Fragments arranged by size:
846 591 481 187 46
BbvCI CC'TCA_GC
Cuts at: 0 1231 1720 2151
Size: 1231 489 431
Fragments arranged by size:
1231 489 431
BglI GCCn_nnn'nGGC
Cuts at: 0 522 1730 2151
Size: 522 1208 421
Fragments arranged by size:
1208 522 421
BlpI GC'TnA_GC
Cuts at: 0 815 1448 2151
Size: 815 633 703
Fragments arranged by size:
815 703 633
Bme1580I G_kGCm'C
Cuts at: 0 181 1586 1790 1889 1926 2151
Size: 181 1405 204 99 37 225
Fragments arranged by size:
1405 225 204 181 99 37
BmgBI CAC'GTC
Cuts at: 0 671 1356 2151
Size: 671 685 795
Fragments arranged by size:
795 685 671
BmrI ACTGGGnnnn_n'
Cuts at: 0 291 1749 2100 2151
Size: 291 1458 351 51
Fragments arranged by size:
1458 351 291 51
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1789 2151
Size: 1789 362
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 868 943 1015 1261 1483 1672 1759
Size: 277 591 75 72 246 222 189 87
Cuts at: 1759 1902 2151
Size: 143 249
Fragments arranged by size:
591 277 249 246 222 189 143 87
75 72
Bpu10I CC'TnA_GC
Cuts at: 0 967 1231 1531 1720 2151
Size: 967 264 300 189 431
Fragments arranged by size:
967 431 300 264 189
BsaI GGTCTCn'nnnn_
Cuts at: 0 1687 1696 2151
Size: 1687 9 455
Fragments arranged by size:
1687 455 9
BsaAI yAC'GTr
Cuts at: 0 420 2057 2151
Size: 420 1637 94
Fragments arranged by size:
1637 420 94
BsaHI Gr'CG_yC
Cuts at: 0 275 596 855 2151
Size: 275 321 259 1296
Fragments arranged by size:
1296 321 275 259
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 321 324 393 1450 1588 1708 2151
Size: 321 3 69 1057 138 120 443
Fragments arranged by size:
1057 443 321 138 120 69 3
BseYI C'CCAG_C
Cuts at: 0 82 162 174 779 1328 1802 2151
Size: 82 80 12 605 549 474 349
Fragments arranged by size:
605 549 474 349 82 80 12
BsiHKAI G_wGCw'C
Cuts at: 0 76 97 253 1219 1300 1586 1790 2151
Size: 76 21 156 966 81 286 204 361
Fragments arranged by size:
966 361 286 204 156 81 76 21
BsmBI CGTCTCn'nnnn_
Cuts at: 0 480 2151
Size: 480 1671
Bsp1286I G_dGCh'C
Cuts at: 0 76 97 181 253 388 490 942 1219
Size: 76 21 84 72 135 102 452 277
Cuts at: 1219 1300 1424 1586 1790 1889 1926 2144 2151
Size: 81 124 162 204 99 37 218 7
Fragments arranged by size:
452 277 218 204 162 135 124 102
99 84 81 76 72 37 21 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 302 1569 1970 2119 2151
Size: 302 1267 401 149 32
Fragments arranged by size:
1267 401 302 149 32
BsrBI CCG'CTC
Cuts at: 0 852 2151
Size: 852 1299
BsrFI r'CCGG_y
Cuts at: 0 521 1255 2151
Size: 521 734 896
Fragments arranged by size:
896 734 521
BssSI C'ACGA_G
Cuts at: 0 226 973 2151
Size: 226 747 1178
Fragments arranged by size:
1178 747 226
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1605 2151
Size: 1605 546
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 955 2151
Size: 912 43 1196
Fragments arranged by size:
1196 912 43
BstYI r'GATC_y
Cuts at: 0 1334 1472 2078 2151
Size: 1334 138 606 73
Fragments arranged by size:
1334 606 138 73
BtgI C'CryG_G
Cuts at: 0 1520 1832 2151
Size: 1520 312 319
Fragments arranged by size:
1520 319 312
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 782 2151
Size: 782 1369
DraI TTT'AAA
Cuts at: 0 105 2151
Size: 105 2046
DraIII CAC_nnn'GTG
Cuts at: 0 1593 2151
Size: 1593 558
DrdI GACnn_nn'nnGTC
Cuts at: 0 1200 2151
Size: 1200 951
EaeI y'GGCC_r
Cuts at: 0 506 945 1253 1508 2051 2151
Size: 506 439 308 255 543 100
Fragments arranged by size:
543 506 439 308 255 100
EarI CTCTTCn'nnn_
Cuts at: 0 124 393 532 1123 1732 2151
Size: 124 269 139 591 609 419
Fragments arranged by size:
609 591 419 269 139 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1321 1828 1846 2151
Size: 1321 507 18 305
Fragments arranged by size:
1321 507 305 18
EcoICRI GAG'CTC
Cuts at: 0 74 251 1217 1298 2151
Size: 74 177 966 81 853
Fragments arranged by size:
966 853 177 81 74
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 513 649 868 943 1015 1048 1192
Size: 277 236 136 219 75 72 33 144
Cuts at: 1192 1261 1309 1411 1483 1672 1759 1902 2109
Size: 69 48 102 72 189 87 143 207
Cuts at: 2109 2151
Size: 42
Fragments arranged by size:
277 236 219 207 189 144 143 136
102 87 75 72 72 69 48 42
33
EcoNI CCTnn'n_nnAGG
Cuts at: 0 129 1030 1264 1966 2151
Size: 129 901 234 702 185
Fragments arranged by size:
901 702 234 185 129
EcoO109I rG'GnC_Cy
Cuts at: 0 122 291 1602 2040 2151
Size: 122 169 1311 438 111
Fragments arranged by size:
1311 438 169 122 111
FseI GG_CCGG'CC
Cuts at: 0 1259 2151
Size: 1259 892
HaeII r_GCGC'y
Cuts at: 0 21 156 169 473 618 991 1614 2151
Size: 21 135 13 304 145 373 623 537
Fragments arranged by size:
623 537 373 304 145 135 21 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1345 1377 1431 1463 2151
Size: 1345 32 54 32 688
Fragments arranged by size:
1345 688 54 32 32
KpnI G_GTAC'C
Cuts at: 0 567 1749 2151
Size: 567 1182 402
Fragments arranged by size:
1182 567 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 911 2151
Size: 911 1240
MscI TGG'CCA
Cuts at: 0 508 947 2053 2151
Size: 508 439 1106 98
Fragments arranged by size:
1106 508 439 98
MslI CAynn'nnrTG
Cuts at: 0 1483 2151
Size: 1483 668
MspA1I CmG'CkG
Cuts at: 0 65 174 347 580 657 1170 1251 1452
Size: 65 109 173 233 77 513 81 201
Cuts at: 1452 1522 2151
Size: 70 629
Fragments arranged by size:
629 513 233 201 173 109 81 77
70 65
NaeI GCC'GGC
Cuts at: 0 523 1257 2151
Size: 523 734 894
Fragments arranged by size:
894 734 523
NgoMIV G'CCGG_C
Cuts at: 0 521 1255 2151
Size: 521 734 896
Fragments arranged by size:
896 734 521
NspI r_CATG'y
Cuts at: 0 1885 2151
Size: 1885 266
PasI CC'CwG_GG
Cuts at: 0 36 2151
Size: 36 2115
PflMI CCAn_nnn'nTGG
Cuts at: 0 2067 2151
Size: 2067 84
PfoI T'CCnGG_A
Cuts at: 0 1064 1208 2151
Size: 1064 144 943
Fragments arranged by size:
1064 943 144
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 2151
Size: 220 32 1899
Fragments arranged by size:
1899 220 32
PpuMI rG'GwC_Cy
Cuts at: 0 122 291 2151
Size: 122 169 1860
Fragments arranged by size:
1860 169 122
PspOMI G'GGCC_C
Cuts at: 0 177 2151
Size: 177 1974
PstI C_TGCA'G
Cuts at: 0 716 1265 1547 1610 1967 2151
Size: 716 549 282 63 357 184
Fragments arranged by size:
716 549 357 282 184 63
PvuII CAG'CTG
Cuts at: 0 174 580 1170 1251 1452 2151
Size: 174 406 590 81 201 699
Fragments arranged by size:
699 590 406 201 174 81
SacI G_AGCT'C
Cuts at: 0 76 253 1219 1300 2151
Size: 76 177 966 81 851
Fragments arranged by size:
966 851 177 81 76
SacII CC_GC'GG
Cuts at: 0 1523 2151
Size: 1523 628
SapI GCTCTTCn'nnn_
Cuts at: 0 393 532 2151
Size: 393 139 1619
Fragments arranged by size:
1619 393 139
SexAI A'CCwGG_T
Cuts at: 0 559 2060 2151
Size: 559 1501 91
Fragments arranged by size:
1501 559 91
SfcI C'TryA_G
Cuts at: 0 207 712 1261 1543 1606 1614 1963 2151
Size: 207 505 549 282 63 8 349 188
Fragments arranged by size:
549 505 349 282 207 188 63 8
SmaI CCC'GGG
Cuts at: 0 835 2151
Size: 835 1316
StuI AGG'CCT
Cuts at: 0 1894 2151
Size: 1894 257
StyI C'CwwG_G
Cuts at: 0 1889 1928 1983 2151
Size: 1889 39 55 168
Fragments arranged by size:
1889 168 55 39
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 258 1495 1805 1917 2151
Size: 258 1237 310 112 234
Fragments arranged by size:
1237 310 258 234 112
TatI w'GTAC_w
Cuts at: 0 2105 2151
Size: 2105 46
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 252 351 955 1799 2061 2151
Size: 252 99 604 844 262 90
Fragments arranged by size:
844 604 262 252 99 90
XmaI C'CCGG_G
Cuts at: 0 833 2151
Size: 833 1318
XmnI GAAnn'nnTTC
Cuts at: 0 634 2151
Size: 634 1517
ZraI GAC'GTC
Cuts at: 0 597 2151
Size: 597 1554
Enzymes that do cut:
AarI AatII Acc65I AcuI AfeI AleI AloI AlwNI
ApaI ApaLI ApoI AvaI BamHI BanI BanII BbsI
BbvCI BglI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaI BsaAI BsaHI BseRI BseYI BsiHKAI BsmBI
Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI
BtgI BtgZI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I KpnI
MmeI MscI MslI MspA1I NaeI NgoMIV NspI PasI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SacII SapI SexAI SfcI SmaI StuI StyI TaqII
TatI XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI AclI AflII AflIII AgeI AhdI AlfI AscI
AseI AsiSI AvrII BaeI BbeI BcgI BciVI BclI
BfrBI BglII BmtI BpuEI BsaBI BsaWI BsaXI BsgI
BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII
BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI EagI
EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI
KasI MfeI MluI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PciI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI SwaI TstI Tth111I XbaI XhoI