Restriction map for
pF1KSDA0646
(Linear) (Six-base) MAPSORT of: KIAA0646.seq Check: 7390 from: 1 to: 1455
KIAA0646.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 518 701 859 955 1027 1455
Size: 395 123 183 158 96 72 428
Fragments arranged by size:
428 395 183 158 123 96 72
AhdI GACnn_n'nnGTC
Cuts at: 0 885 1332 1455
Size: 885 447 123
Fragments arranged by size:
885 447 123
AlwNI CAG_nnn'CTG
Cuts at: 0 490 1029 1265 1455
Size: 490 539 236 190
Fragments arranged by size:
539 490 236 190
ApoI r'AATT_y
Cuts at: 0 463 510 1455
Size: 463 47 945
Fragments arranged by size:
945 463 47
AseI AT'TA_AT
Cuts at: 0 1370 1455
Size: 1370 85
BamHI G'GATC_C
Cuts at: 0 609 1455
Size: 609 846
BanI G'GyrC_C
Cuts at: 0 53 1455
Size: 53 1402
BanII G_rGCy'C
Cuts at: 0 11 654 1047 1202 1396 1455
Size: 11 643 393 155 194 59
Fragments arranged by size:
643 393 194 155 59 11
BbsI GAAGACnn'nnnn_
Cuts at: 0 386 949 1455
Size: 386 563 506
Fragments arranged by size:
563 506 386
BlpI GC'TnA_GC
Cuts at: 0 911 1455
Size: 911 544
Bme1580I G_kGCm'C
Cuts at: 0 1257 1455
Size: 1257 198
BmrI ACTGGGnnnn_n'
Cuts at: 0 393 1455
Size: 393 1062
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 512 1455
Size: 512 943
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 1455
Size: 649 806
BsaI GGTCTCn'nnnn_
Cuts at: 0 23 1455
Size: 23 1432
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 636 666 1455
Size: 636 30 789
Fragments arranged by size:
789 636 30
BseYI C'CCAG_C
Cuts at: 0 890 1455
Size: 890 565
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 920 1455
Size: 920 535
BsiHKAI G_wGCw'C
Cuts at: 0 1202 1396 1455
Size: 1202 194 59
Fragments arranged by size:
1202 194 59
BsmI GAATG_Cn'
Cuts at: 0 354 1312 1455
Size: 354 958 143
Fragments arranged by size:
958 354 143
Bsp1286I G_dGCh'C
Cuts at: 0 11 654 1047 1202 1257 1396 1455
Size: 11 643 393 155 55 139 59
Fragments arranged by size:
643 393 155 139 59 55 11
BspHI T'CATG_A
Cuts at: 0 693 1455
Size: 693 762
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 475 1455
Size: 475 980
BsrFI r'CCGG_y
Cuts at: 0 37 1455
Size: 37 1418
BstEII G'GTnAC_C
Cuts at: 0 750 1455
Size: 750 705
BstYI r'GATC_y
Cuts at: 0 609 1455
Size: 609 846
Bsu36I CC'TnA_GG
Cuts at: 0 209 1455
Size: 209 1246
EaeI y'GGCC_r
Cuts at: 0 42 584 1455
Size: 42 542 871
Fragments arranged by size:
871 542 42
EarI CTCTTCn'nnn_
Cuts at: 0 720 950 1049 1124 1455
Size: 720 230 99 75 331
Fragments arranged by size:
720 331 230 99 75
EcoICRI GAG'CTC
Cuts at: 0 1200 1394 1455
Size: 1200 194 61
Fragments arranged by size:
1200 194 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 395 512 518 701 859 955 1027 1455
Size: 395 117 6 183 158 96 72 428
Fragments arranged by size:
428 395 183 158 117 96 72 6
EcoO109I rG'GnC_Cy
Cuts at: 0 487 502 1002 1455
Size: 487 15 500 453
Fragments arranged by size:
500 487 453 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1095 1183 1455
Size: 1095 88 272
Fragments arranged by size:
1095 272 88
HaeII r_GCGC'y
Cuts at: 0 80 1455
Size: 80 1375
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 119 151 162 194 299 331 381 413
Size: 119 32 11 32 105 32 50 32
Cuts at: 413 871 903 1318 1350 1455
Size: 458 32 415 32 105
Fragments arranged by size:
458 415 119 105 105 50 32 32
32 32 32 32 11
HincII GTy'rAC
Cuts at: 0 620 1455
Size: 620 835
HindIII A'AGCT_T
Cuts at: 0 732 1455
Size: 732 723
MfeI C'AATT_G
Cuts at: 0 1362 1455
Size: 1362 93
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 342 1300 1455
Size: 342 958 155
Fragments arranged by size:
958 342 155
MscI TGG'CCA
Cuts at: 0 586 1455
Size: 586 869
MslI CAynn'nnrTG
Cuts at: 0 1178 1455
Size: 1178 277
MspA1I CmG'CkG
Cuts at: 0 894 1017 1455
Size: 894 123 438
Fragments arranged by size:
894 438 123
PfoI T'CCnGG_A
Cuts at: 0 489 760 950 1455
Size: 489 271 190 505
Fragments arranged by size:
505 489 271 190
PpuMI rG'GwC_Cy
Cuts at: 0 487 1002 1455
Size: 487 515 453
Fragments arranged by size:
515 487 453
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1200 1232 1455
Size: 1200 32 223
Fragments arranged by size:
1200 223 32
PstI C_TGCA'G
Cuts at: 0 1034 1455
Size: 1034 421
PvuII CAG'CTG
Cuts at: 0 894 1017 1455
Size: 894 123 438
Fragments arranged by size:
894 438 123
SacI G_AGCT'C
Cuts at: 0 1202 1396 1455
Size: 1202 194 59
Fragments arranged by size:
1202 194 59
SapI GCTCTTCn'nnn_
Cuts at: 0 1049 1455
Size: 1049 406
SfcI C'TryA_G
Cuts at: 0 116 1030 1455
Size: 116 914 425
Fragments arranged by size:
914 425 116
SgrAI Cr'CCGG_yG
Cuts at: 0 37 1455
Size: 37 1418
SmlI C'TyrA_G
Cuts at: 0 628 1455
Size: 628 827
StyI C'CwwG_G
Cuts at: 0 990 1124 1455
Size: 990 134 331
Fragments arranged by size:
990 331 134
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 1455
Size: 92 1363
TatI w'GTAC_w
Cuts at: 0 589 1455
Size: 589 866
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 88 120 636 668 1455
Size: 88 32 516 32 787
Fragments arranged by size:
787 516 88 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 756 1455
Size: 756 699
XmnI GAAnn'nnTTC
Cuts at: 0 946 1163 1455
Size: 946 217 292
Fragments arranged by size:
946 292 217
Enzymes that do cut:
AcuI AhdI AlwNI ApoI AseI BamHI BanI BanII
BbsI BlpI Bme1580I BmrI BpmI BpuEI BsaI BsaXI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI
BstEII BstYI Bsu36I EaeI EarI EcoICRI Eco57MI EcoO109I
FalI HaeII Hin4I HincII HindIII MfeI MmeI MscI
MslI MspA1I PfoI PpuMI PsrI PstI PvuII SacI
SapI SfcI SgrAI SmlI StyI TaqII TatI TstI
Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaI ApaLI AscI AsiSI
AvaI AvrII BaeI BbeI BbvCI BcgI BciVI BclI
BfrBI BglI BglII BmgBI BmtI BplI Bpu10I BsaAI
BsaBI BsaHI BsaWI BseRI BsiEI BsiWI BsmBI BspEI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EagI EciI EcoNI EcoRI EcoRV FseI FspI
FspAI HpaI KasI KpnI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PvuI RsrII SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SmaI SnaBI SpeI
SphI SrfI SspI StuI SwaI XbaI XcmI XhoI
XmaI ZraI