Restriction map for
pF1KSDA0634
(Linear) (Six-base) MAPSORT of: KIAA0634.seq Check: 6705 from: 1 to: 3864
KIAA0634.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1854 2599 2978 3864
Size: 1854 745 379 886
Fragments arranged by size:
1854 886 745 379
AatII G_ACGT'C
Cuts at: 0 1048 3864
Size: 1048 2816
AccI GT'mk_AC
Cuts at: 0 1600 3864
Size: 1600 2264
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 3308 3864
Size: 214 3094 556
Fragments arranged by size:
3094 556 214
AflIII A'CryG_T
Cuts at: 0 3805 3839 3864
Size: 3805 34 25
Fragments arranged by size:
3805 34 25
AhdI GACnn_n'nnGTC
Cuts at: 0 1782 3413 3864
Size: 1782 1631 451
Fragments arranged by size:
1782 1631 451
AleI CACnn'nnGTG
Cuts at: 0 1490 2851 3864
Size: 1490 1361 1013
Fragments arranged by size:
1490 1361 1013
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 412 1141 3864
Size: 412 729 2723
Fragments arranged by size:
2723 729 412
AlwNI CAG_nnn'CTG
Cuts at: 0 495 1505 2004 2631 2736 3187 3864
Size: 495 1010 499 627 105 451 677
Fragments arranged by size:
1010 677 627 499 495 451 105
ApaI G_GGCC'C
Cuts at: 0 2125 3626 3864
Size: 2125 1501 238
Fragments arranged by size:
2125 1501 238
ApaLI G'TGCA_C
Cuts at: 0 475 2930 3864
Size: 475 2455 934
Fragments arranged by size:
2455 934 475
ApoI r'AATT_y
Cuts at: 0 2149 3205 3864
Size: 2149 1056 659
Fragments arranged by size:
2149 1056 659
AvaI C'yCGr_G
Cuts at: 0 39 75 312 1249 1875 3864
Size: 39 36 237 937 626 1989
Fragments arranged by size:
1989 937 626 237 39 36
AvrII C'CTAG_G
Cuts at: 0 904 3864
Size: 904 2960
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 3471 3504 3864
Size: 3471 33 360
Fragments arranged by size:
3471 360 33
BanI G'GyrC_C
Cuts at: 0 13 148 253 334 505 2877 3864
Size: 13 135 105 81 171 2372 987
Fragments arranged by size:
2372 987 171 135 105 81 13
BanII G_rGCy'C
Cuts at: 0 47 176 319 581 1054 2125 2342 2588
Size: 47 129 143 262 473 1071 217 246
Cuts at: 2588 2684 2845 3023 3080 3329 3626 3864
Size: 96 161 178 57 249 297 238
Fragments arranged by size:
1071 473 297 262 249 246 238 217
178 161 143 129 96 57 47
BbeI G_GCGC'C
Cuts at: 0 17 152 257 3864
Size: 17 135 105 3607
Fragments arranged by size:
3607 135 105 17
BbsI GAAGACnn'nnnn_
Cuts at: 0 202 1724 1778 2217 3864
Size: 202 1522 54 439 1647
Fragments arranged by size:
1647 1522 439 202 54
BbvCI CC'TCA_GC
Cuts at: 0 2998 3864
Size: 2998 866
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 688 722 3864
Size: 688 34 3142
Fragments arranged by size:
3142 688 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 3357 3864
Size: 3357 507
BglI GCCn_nnn'nGGC
Cuts at: 0 906 2825 3864
Size: 906 1919 1039
Fragments arranged by size:
1919 1039 906
BglII A'GATC_T
Cuts at: 0 3458 3864
Size: 3458 406
BlpI GC'TnA_GC
Cuts at: 0 22 2449 2839 3388 3864
Size: 22 2427 390 549 476
Fragments arranged by size:
2427 549 476 390 22
Bme1580I G_kGCm'C
Cuts at: 0 479 848 1250 1891 2125 2934 3247 3626
Size: 479 369 402 641 234 809 313 379
Cuts at: 3626 3728 3864
Size: 102 136
Fragments arranged by size:
809 641 479 402 379 369 313 234
136 102
BmgBI CAC'GTC
Cuts at: 0 522 3864
Size: 522 3342
BmrI ACTGGGnnnn_n'
Cuts at: 0 1132 1515 3864
Size: 1132 383 2349
Fragments arranged by size:
2349 1132 383
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 460 501 517 556 2387 2623 3066
Size: 267 193 41 16 39 1831 236 443
Cuts at: 3066 3343 3621 3663 3676 3864
Size: 277 278 42 13 188
Fragments arranged by size:
1831 443 278 277 267 236 193 188
42 41 39 16 13
Bpu10I CC'TnA_GC
Cuts at: 0 2955 2998 3864
Size: 2955 43 866
Fragments arranged by size:
2955 866 43
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1564 2473 2668 3772 3864
Size: 1564 909 195 1104 92
Fragments arranged by size:
1564 1104 909 195 92
BsaI GGTCTCn'nnnn_
Cuts at: 0 2647 3099 3144 3340 3534 3864
Size: 2647 452 45 196 194 330
Fragments arranged by size:
2647 452 330 196 194 45
BsaAI yAC'GTr
Cuts at: 0 3806 3842 3864
Size: 3806 36 22
Fragments arranged by size:
3806 36 22
BsaBI GATnn'nnATC
Cuts at: 0 607 3864
Size: 607 3257
BsaHI Gr'CG_yC
Cuts at: 0 14 149 254 1045 3864
Size: 14 135 105 791 2819
Fragments arranged by size:
2819 791 135 105 14
BsaWI w'CCGG_w
Cuts at: 0 1435 2195 2346 2423 3864
Size: 1435 760 151 77 1441
Fragments arranged by size:
1441 1435 760 151 77
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 907 937 1921 1951 2302 2332 2512 2533
Size: 907 30 984 30 351 30 180 21
Cuts at: 2533 2542 2563 2671 2701 3864
Size: 9 21 108 30 1163
Fragments arranged by size:
1163 984 907 351 180 108 30 30
30 30 21 21 9
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 610 901 910 1909 2077 2326 2533 2794
Size: 610 291 9 999 168 249 207 261
Cuts at: 2794 3763 3826 3864
Size: 969 63 38
Fragments arranged by size:
999 969 610 291 261 249 207 168
63 38 9
BseYI C'CCAG_C
Cuts at: 0 274 686 777 2201 2270 2608 2664 3618
Size: 274 412 91 1424 69 338 56 954
Cuts at: 3618 3864
Size: 246
Fragments arranged by size:
1424 954 412 338 274 246 91 69
56
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 418 1464 1815 2428 3006 3864
Size: 418 1046 351 613 578 858
Fragments arranged by size:
1046 858 613 578 418 351
BsiEI CG_ry'CG
Cuts at: 0 58 3864
Size: 58 3806
BsiHKAI G_wGCw'C
Cuts at: 0 479 1256 1709 2035 2588 2684 2934 2956
Size: 479 777 453 326 553 96 250 22
Cuts at: 2956 3864
Size: 908
Fragments arranged by size:
908 777 553 479 453 326 250 96
22
BsiWI C'GTAC_G
Cuts at: 0 3842 3864
Size: 3842 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 528 3864
Size: 528 3336
Bsp1286I G_dGCh'C
Cuts at: 0 47 176 319 479 581 848 1054 1250
Size: 47 129 143 160 102 267 206 196
Cuts at: 1250 1256 1709 1891 2035 2125 2342 2588 2684
Size: 6 453 182 144 90 217 246 96
Cuts at: 2684 2845 2934 2956 3023 3080 3247 3329 3626
Size: 161 89 22 67 57 167 82 297
Cuts at: 3626 3728 3864
Size: 102 136
Fragments arranged by size:
453 297 267 246 217 206 196 182
167 161 160 144 143 136 129 102
102 96 90 89 82 67 57 47
22 6
BspEI T'CCGG_A
Cuts at: 0 2346 2423 3864
Size: 2346 77 1441
Fragments arranged by size:
2346 1441 77
BspHI T'CATG_A
Cuts at: 0 747 1452 3864
Size: 747 705 2412
Fragments arranged by size:
2412 747 705
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1787 1854 2599 2978 3864
Size: 1787 67 745 379 886
Fragments arranged by size:
1787 886 745 379 67
BsrBI CCG'CTC
Cuts at: 0 251 3864
Size: 251 3613
BsrFI r'CCGG_y
Cuts at: 0 10 145 188 331 1859 2304 2819 3048
Size: 10 135 43 143 1528 445 515 229
Cuts at: 3048 3864
Size: 816
Fragments arranged by size:
1528 816 515 445 229 143 135 43
10
BsrGI T'GTAC_A
Cuts at: 0 3194 3470 3864
Size: 3194 276 394
Fragments arranged by size:
3194 394 276
BssSI C'ACGA_G
Cuts at: 0 3368 3864
Size: 3368 496
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1952 2004 3051 3864
Size: 1952 52 1047 813
Fragments arranged by size:
1952 1047 813 52
BstEII G'GTnAC_C
Cuts at: 0 951 2395 3396 3864
Size: 951 1444 1001 468
Fragments arranged by size:
1444 1001 951 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2545 2664 2905 3090 3864
Size: 2545 119 241 185 774
Fragments arranged by size:
2545 774 241 185 119
BstYI r'GATC_y
Cuts at: 0 752 2492 3458 3864
Size: 752 1740 966 406
Fragments arranged by size:
1740 966 752 406
Bsu36I CC'TnA_GG
Cuts at: 0 1892 3403 3864
Size: 1892 1511 461
Fragments arranged by size:
1892 1511 461
BtgI C'CryG_G
Cuts at: 0 671 1190 1369 2390 2402 3273 3864
Size: 671 519 179 1021 12 871 591
Fragments arranged by size:
1021 871 671 591 519 179 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 375 624 1759 2544 3864
Size: 375 249 1135 785 1320
Fragments arranged by size:
1320 1135 785 375 249
BtsI GCAGTG_nn'
Cuts at: 0 88 214 496 1506 2323 2419 3672 3864
Size: 88 126 282 1010 817 96 1253 192
Fragments arranged by size:
1253 1010 817 282 192 126 96 88
ClaI AT'CG_AT
Cuts at: 0 2975 3864
Size: 2975 889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1743 1778 3864
Size: 1743 35 2086
Fragments arranged by size:
2086 1743 35
EaeI y'GGCC_r
Cuts at: 0 2 55 143 1187 1328 1685 2276 2821
Size: 2 53 88 1044 141 357 591 545
Cuts at: 2821 3864
Size: 1043
Fragments arranged by size:
1044 1043 591 545 357 141 88 53
2
EagI C'GGCC_G
Cuts at: 0 55 3864
Size: 55 3809
EarI CTCTTCn'nnn_
Cuts at: 0 718 983 3864
Size: 718 265 2881
Fragments arranged by size:
2881 718 265
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 475 1646 3864
Size: 475 1171 2218
Fragments arranged by size:
2218 1171 475
EcoICRI GAG'CTC
Cuts at: 0 2586 2682 3864
Size: 2586 96 1182
Fragments arranged by size:
2586 1182 96
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 267 460 501 517 556 2387 2623
Size: 214 53 193 41 16 39 1831 236
Cuts at: 2623 3066 3308 3343 3621 3663 3676 3864
Size: 443 242 35 278 42 13 188
Fragments arranged by size:
1831 443 278 242 236 214 193 188
53 42 41 39 35 16 13
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2500 3864
Size: 2500 1364
EcoO109I rG'GnC_Cy
Cuts at: 0 781 1380 1522 2121 2122 2328 2568 3282
Size: 781 599 142 599 1 206 240 714
Cuts at: 3282 3622 3864
Size: 340 242
Fragments arranged by size:
781 714 599 599 340 242 240 206
142 1
EcoRI G'AATT_C
Cuts at: 0 3205 3864
Size: 3205 659
EcoRV GAT'ATC
Cuts at: 0 2373 3864
Size: 2373 1491
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1017 2937 3864
Size: 1017 1920 927
Fragments arranged by size:
1920 1017 927
FspI TGC'GCA
Cuts at: 0 1498 3864
Size: 1498 2366
HaeII r_GCGC'y
Cuts at: 0 17 152 257 679 2793 3005 3864
Size: 17 135 105 422 2114 212 859
Fragments arranged by size:
2114 859 422 212 135 105 17
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 945 977 1200 1232 1768 1800 2074 2106
Size: 945 32 223 32 536 32 274 32
Cuts at: 2106 2512 2544 2671 2703 3217 3249 3399 3431
Size: 406 32 127 32 514 32 150 32
Cuts at: 3431 3450 3482 3864
Size: 19 32 382
Fragments arranged by size:
945 536 514 406 382 274 223 150
127 32 32 32 32 32 32 32
32 32 19
HindIII A'AGCT_T
Cuts at: 0 1333 1644 3864
Size: 1333 311 2220
Fragments arranged by size:
2220 1333 311
KasI G'GCGC_C
Cuts at: 0 13 148 253 3864
Size: 13 135 105 3611
Fragments arranged by size:
3611 135 105 13
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1337 2379 3167 3864
Size: 1337 1042 788 697
Fragments arranged by size:
1337 1042 788 697
MscI TGG'CCA
Cuts at: 0 1189 1687 3864
Size: 1189 498 2177
Fragments arranged by size:
2177 1189 498
MslI CAynn'nnrTG
Cuts at: 0 394 622 841 1490 2041 2851 3520 3864
Size: 394 228 219 649 551 810 669 344
Fragments arranged by size:
810 669 649 551 394 344 228 219
MspA1I CmG'CkG
Cuts at: 0 99 138 160 457 546 1077 1448 1691
Size: 99 39 22 297 89 531 371 243
Cuts at: 1691 1986 2274 2301 2631 2838 3262 3864
Size: 295 288 27 330 207 424 602
Fragments arranged by size:
602 531 424 371 330 297 295 288
243 207 99 89 39 27 22
NaeI GCC'GGC
Cuts at: 0 12 147 190 333 2821 3864
Size: 12 135 43 143 2488 1043
Fragments arranged by size:
2488 1043 143 135 43 12
NarI GG'CG_CC
Cuts at: 0 14 149 254 3864
Size: 14 135 105 3610
Fragments arranged by size:
3610 135 105 14
NcoI C'CATG_G
Cuts at: 0 1190 1369 2390 2402 3273 3864
Size: 1190 179 1021 12 871 591
Fragments arranged by size:
1190 1021 871 591 179 12
NgoMIV G'CCGG_C
Cuts at: 0 10 145 188 331 2819 3864
Size: 10 135 43 143 2488 1045
Fragments arranged by size:
2488 1045 143 135 43 10
NspI r_CATG'y
Cuts at: 0 1246 2209 3864
Size: 1246 963 1655
Fragments arranged by size:
1655 1246 963
PflMI CCAn_nnn'nTGG
Cuts at: 0 963 3127 3864
Size: 963 2164 737
Fragments arranged by size:
2164 963 737
PfoI T'CCnGG_A
Cuts at: 0 572 851 866 3864
Size: 572 279 15 2998
Fragments arranged by size:
2998 572 279 15
PmlI CAC'GTG
Cuts at: 0 3806 3864
Size: 3806 58
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 821 3864
Size: 789 32 3043
Fragments arranged by size:
3043 789 32
PpuMI rG'GwC_Cy
Cuts at: 0 781 1380 1522 2328 3282 3864
Size: 781 599 142 806 954 582
Fragments arranged by size:
954 806 781 599 582 142
PshAI GACnn'nnGTC
Cuts at: 0 3136 3864
Size: 3136 728
PspOMI G'GGCC_C
Cuts at: 0 2121 3622 3864
Size: 2121 1501 242
Fragments arranged by size:
2121 1501 242
PstI C_TGCA'G
Cuts at: 0 1728 1997 3034 3047 3158 3765 3864
Size: 1728 269 1037 13 111 607 99
Fragments arranged by size:
1728 1037 607 269 111 99 13
PvuII CAG'CTG
Cuts at: 0 546 1077 1448 1691 1986 2274 2631 2838
Size: 546 531 371 243 295 288 357 207
Cuts at: 2838 3262 3864
Size: 424 602
Fragments arranged by size:
602 546 531 424 371 357 295 288
243 207
SacI G_AGCT'C
Cuts at: 0 2588 2684 3864
Size: 2588 96 1180
Fragments arranged by size:
2588 1180 96
SanDI GG'GwC_CC
Cuts at: 0 781 3864
Size: 781 3083
SapI GCTCTTCn'nnn_
Cuts at: 0 718 3864
Size: 718 3146
SbfI CC_TGCA'GG
Cuts at: 0 3765 3864
Size: 3765 99
ScaI AGT'ACT
Cuts at: 0 3087 3864
Size: 3087 777
SfcI C'TryA_G
Cuts at: 0 1724 1993 3030 3043 3154 3761 3864
Size: 1724 269 1037 13 111 607 103
Fragments arranged by size:
1724 1037 607 269 111 103 13
SfoI GGC'GCC
Cuts at: 0 15 150 255 3864
Size: 15 135 105 3609
Fragments arranged by size:
3609 135 105 15
SgrAI Cr'CCGG_yG
Cuts at: 0 10 3864
Size: 10 3854
SmaI CCC'GGG
Cuts at: 0 77 314 3864
Size: 77 237 3550
Fragments arranged by size:
3550 237 77
SmlI C'TyrA_G
Cuts at: 0 1543 2488 2683 3787 3864
Size: 1543 945 195 1104 77
Fragments arranged by size:
1543 1104 945 195 77
StuI AGG'CCT
Cuts at: 0 1622 2920 3864
Size: 1622 1298 944
Fragments arranged by size:
1622 1298 944
StyI C'CwwG_G
Cuts at: 0 904 1037 1190 1369 1525 1617 2214 2390
Size: 904 133 153 179 156 92 597 176
Cuts at: 2390 2402 2501 3098 3134 3273 3854 3864
Size: 12 99 597 36 139 581 10
Fragments arranged by size:
904 597 597 581 179 176 156 153
139 133 99 92 36 12 10
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 619 1362 1714 3096 3864
Size: 619 743 352 1382 768
Fragments arranged by size:
1382 768 743 619 352
TatI w'GTAC_w
Cuts at: 0 512 2066 3085 3194 3470 3864
Size: 512 1554 1019 109 276 394
Fragments arranged by size:
1554 1019 512 394 276 109
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2302 2334 2533 2565 3864
Size: 2302 32 199 32 1299
Fragments arranged by size:
2302 1299 199 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 3595 3864
Size: 3595 269
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1680 2278 2362 3864
Size: 1680 598 84 1502
Fragments arranged by size:
1680 1502 598 84
XmaI C'CCGG_G
Cuts at: 0 75 312 3864
Size: 75 237 3552
Fragments arranged by size:
3552 237 75
XmnI GAAnn'nnTTC
Cuts at: 0 1589 2251 3864
Size: 1589 662 1613
Fragments arranged by size:
1613 1589 662
ZraI GAC'GTC
Cuts at: 0 1046 3864
Size: 1046 2818
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AhdI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI AvrII BaeI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HindIII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NspI PflMI
PfoI PmlI PpiI PpuMI PshAI PspOMI PstI PvuII
SacI SanDI SapI SbfI ScaI SfcI SfoI SgrAI
SmaI SmlI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AloI AscI AseI
AsiSI BamHI BclI BfrBI BmtI BplI BsmI BsrDI
BssHII BstBI BstZ17I DraI DraIII DrdI FseI FspAI
HincII HpaI KpnI MfeI MluI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PsiI PspXI
PsrI PvuI RsrII SacII SalI SexAI SfiI SgfI
SnaBI SpeI SphI SrfI SspI SwaI XbaI XhoI