Restriction map for
pF1KSDA0617
(Linear) (Six-base) MAPSORT of: KIAA0617.seq Check: 4235 from: 1 to: 2304
KIAA0617.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AccI GT'mk_AC
Cuts at: 0 416 533 1635 2084 2288 2304
Size: 416 117 1102 449 204 16
Fragments arranged by size:
1102 449 416 204 117 16
AclI AA'CG_TT
Cuts at: 0 1287 2304
Size: 1287 1017
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1010 1063 1739 2304
Size: 674 336 53 676 565
Fragments arranged by size:
676 674 565 336 53
AleI CACnn'nnGTG
Cuts at: 0 1560 2304
Size: 1560 744
AlwNI CAG_nnn'CTG
Cuts at: 0 2032 2304
Size: 2032 272
ApaLI G'TGCA_C
Cuts at: 0 1736 2304
Size: 1736 568
ApoI r'AATT_y
Cuts at: 0 282 643 667 840 1456 1844 2304
Size: 282 361 24 173 616 388 460
Fragments arranged by size:
616 460 388 361 282 173 24
AseI AT'TA_AT
Cuts at: 0 430 722 1804 2304
Size: 430 292 1082 500
Fragments arranged by size:
1082 500 430 292
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1469 1502 2304
Size: 1469 33 802
Fragments arranged by size:
1469 802 33
BamHI G'GATC_C
Cuts at: 0 50 2304
Size: 50 2254
BanII G_rGCy'C
Cuts at: 0 170 1890 2304
Size: 170 1720 414
Fragments arranged by size:
1720 414 170
BbsI GAAGACnn'nnnn_
Cuts at: 0 826 887 2304
Size: 826 61 1417
Fragments arranged by size:
1417 826 61
BclI T'GATC_A
Cuts at: 0 330 1986 2304
Size: 330 1656 318
Fragments arranged by size:
1656 330 318
BfrBI ATG'CAT
Cuts at: 0 181 2304
Size: 181 2123
BglII A'GATC_T
Cuts at: 0 1671 2304
Size: 1671 633
Bme1580I G_kGCm'C
Cuts at: 0 1740 2304
Size: 1740 564
BmgBI CAC'GTC
Cuts at: 0 1400 2304
Size: 1400 904
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 232 2304
Size: 232 2072
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1128 1633 2304
Size: 1128 505 671
Fragments arranged by size:
1128 671 505
BsaI GGTCTCn'nnnn_
Cuts at: 0 586 1154 2304
Size: 586 568 1150
Fragments arranged by size:
1150 586 568
BsaBI GATnn'nnATC
Cuts at: 0 49 2304
Size: 49 2255
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 515 545 1126 1156 2304
Size: 515 30 581 30 1148
Fragments arranged by size:
1148 581 515 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 178 2304
Size: 178 2126
BsiEI CG_ry'CG
Cuts at: 0 1059 2304
Size: 1059 1245
BsiHKAI G_wGCw'C
Cuts at: 0 170 1523 1740 2304
Size: 170 1353 217 564
Fragments arranged by size:
1353 564 217 170
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1096 2304
Size: 1096 1208
Bsp1286I G_dGCh'C
Cuts at: 0 170 1523 1740 1890 2304
Size: 170 1353 217 150 414
Fragments arranged by size:
1353 414 217 170 150
BsrBI CCG'CTC
Cuts at: 0 516 2304
Size: 516 1788
BsrDI GCAATG_nn'
Cuts at: 0 1250 2304
Size: 1250 1054
BsrGI T'GTAC_A
Cuts at: 0 371 390 896 2304
Size: 371 19 506 1408
Fragments arranged by size:
1408 506 371 19
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1773 2304
Size: 1773 531
BstYI r'GATC_y
Cuts at: 0 50 1038 1671 1714 2304
Size: 50 988 633 43 590
Fragments arranged by size:
988 633 590 50 43
BstZ17I GTA'TAC
Cuts at: 0 2289 2304
Size: 2289 15
Bsu36I CC'TnA_GG
Cuts at: 0 2273 2304
Size: 2273 31
BtgI C'CryG_G
Cuts at: 0 71 2304
Size: 71 2233
BtsI GCAGTG_nn'
Cuts at: 0 747 2304
Size: 747 1557
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1611 1646 2304
Size: 1611 35 658
Fragments arranged by size:
1611 658 35
DraI TTT'AAA
Cuts at: 0 1098 2304
Size: 1098 1206
EarI CTCTTCn'nnn_
Cuts at: 0 1039 2304
Size: 1039 1265
EcoICRI GAG'CTC
Cuts at: 0 168 2304
Size: 168 2136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 674 1010 1063 1128 1633 1739 2304
Size: 674 336 53 65 505 106 565
Fragments arranged by size:
674 565 505 336 106 65 53
EcoNI CCTnn'n_nnAGG
Cuts at: 0 960 2304
Size: 960 1344
EcoO109I rG'GnC_Cy
Cuts at: 0 102 2304
Size: 102 2202
EcoRI G'AATT_C
Cuts at: 0 840 1456 2304
Size: 840 616 848
Fragments arranged by size:
848 840 616
EcoRV GAT'ATC
Cuts at: 0 1364 1863 2304
Size: 1364 499 441
Fragments arranged by size:
1364 499 441
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 572 1607 2304
Size: 572 1035 697
Fragments arranged by size:
1035 697 572
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 564 596 2304
Size: 564 32 1708
Fragments arranged by size:
1708 564 32
HincII GTy'rAC
Cuts at: 0 1017 1636 2304
Size: 1017 619 668
Fragments arranged by size:
1017 668 619
HindIII A'AGCT_T
Cuts at: 0 320 2304
Size: 320 1984
MfeI C'AATT_G
Cuts at: 0 776 2304
Size: 776 1528
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1710 2075 2304
Size: 1710 365 229
Fragments arranged by size:
1710 365 229
MslI CAynn'nnrTG
Cuts at: 0 43 203 367 1447 1560 1804 2304
Size: 43 160 164 1080 113 244 500
Fragments arranged by size:
1080 500 244 164 160 113 43
MspA1I CmG'CkG
Cuts at: 0 1923 2304
Size: 1923 381
NcoI C'CATG_G
Cuts at: 0 71 2304
Size: 71 2233
NdeI CA'TA_TG
Cuts at: 0 857 1658 2295 2304
Size: 857 801 637 9
Fragments arranged by size:
857 801 637 9
NsiI A_TGCA'T
Cuts at: 0 183 2304
Size: 183 2121
NspI r_CATG'y
Cuts at: 0 897 1591 2304
Size: 897 694 713
Fragments arranged by size:
897 713 694
PfoI T'CCnGG_A
Cuts at: 0 1067 2304
Size: 1067 1237
PpuMI rG'GwC_Cy
Cuts at: 0 102 2304
Size: 102 2202
PsiI TTA'TAA
Cuts at: 0 1295 2304
Size: 1295 1009
PstI C_TGCA'G
Cuts at: 0 642 1671 2304
Size: 642 1029 633
Fragments arranged by size:
1029 642 633
PvuI CG_AT'CG
Cuts at: 0 1059 2304
Size: 1059 1245
SacI G_AGCT'C
Cuts at: 0 170 2304
Size: 170 2134
SalI G'TCGA_C
Cuts at: 0 1634 2304
Size: 1634 670
SfcI C'TryA_G
Cuts at: 0 171 638 824 1471 1667 1891 2117 2304
Size: 171 467 186 647 196 224 226 187
Fragments arranged by size:
647 467 226 224 196 187 186 171
SspI AAT'ATT
Cuts at: 0 1232 2257 2304
Size: 1232 1025 47
Fragments arranged by size:
1232 1025 47
StyI C'CwwG_G
Cuts at: 0 71 885 2304
Size: 71 814 1419
Fragments arranged by size:
1419 814 71
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 452 2304
Size: 452 1852
TatI w'GTAC_w
Cuts at: 0 276 371 390 852 896 2304
Size: 276 95 19 462 44 1408
Fragments arranged by size:
1408 462 276 95 44 19
XbaI T'CTAG_A
Cuts at: 0 2137 2304
Size: 2137 167
XmnI GAAnn'nnTTC
Cuts at: 0 647 2304
Size: 647 1657
Enzymes that do cut:
AccI AclI AcuI AleI AlwNI ApaLI ApoI AseI
BaeI BamHI BanII BbsI BclI BfrBI BglII Bme1580I
BmgBI BplI BpmI BsaI BsaBI BsaXI BseRI BsiEI
BsiHKAI BsmBI Bsp1286I BsrBI BsrDI BsrGI BstAPI BstYI
BstZ17I Bsu36I BtgI BtsI CspCI DraI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI Hin4I HincII
HindIII MfeI MmeI MslI MspA1I NcoI NdeI NsiI
NspI PfoI PpuMI PsiI PstI PvuI SacI SalI
SfcI SspI StyI TaqII TatI XbaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaI AscI AsiSI AvaI AvrII BanI
BbeI BbvCI BcgI BciVI BglI BlpI BmrI BmtI
Bpu10I BpuEI BsaAI BsaHI BsaWI BseYI BsgI BsiWI
BsmI BspEI BspHI BspMI BsrFI BssHII BssSI BstBI
BstEII BstXI BtgZI ClaI DraIII DrdI EaeI EagI
EciI FseI FspI FspAI HaeII HpaI KasI KpnI
MluI MscI NaeI NarI NgoMIV NheI NotI NruI
PacI PasI PciI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI StuI SwaI TstI
Tth111I XcmI XhoI XmaI ZraI