Restriction map for
pF1KSDA0585
(Linear) (Six-base) MAPSORT of: KIAA0585.seq Check: 6737 from: 1 to: 1242
KIAA0585.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 979 1242
Size: 979 263
AfeI AGC'GCT
Cuts at: 0 613 1242
Size: 613 629
AlwNI CAG_nnn'CTG
Cuts at: 0 584 1242
Size: 584 658
ApaLI G'TGCA_C
Cuts at: 0 1111 1242
Size: 1111 131
ApoI r'AATT_y
Cuts at: 0 172 751 858 1242
Size: 172 579 107 384
Fragments arranged by size:
579 384 172 107
AvaI C'yCGr_G
Cuts at: 0 371 662 961 1242
Size: 371 291 299 281
Fragments arranged by size:
371 299 291 281
BamHI G'GATC_C
Cuts at: 0 1090 1242
Size: 1090 152
BanI G'GyrC_C
Cuts at: 0 453 617 1242
Size: 453 164 625
Fragments arranged by size:
625 453 164
BanII G_rGCy'C
Cuts at: 0 59 115 1236 1242
Size: 59 56 1121 6
Fragments arranged by size:
1121 59 56 6
BbsI GAAGACnn'nnnn_
Cuts at: 0 449 1242
Size: 449 793
BlpI GC'TnA_GC
Cuts at: 0 109 1242
Size: 109 1133
Bme1580I G_kGCm'C
Cuts at: 0 1115 1242
Size: 1115 127
BmgBI CAC'GTC
Cuts at: 0 1136 1242
Size: 1136 106
BmrI ACTGGGnnnn_n'
Cuts at: 0 560 1242
Size: 560 682
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 1215 1242
Size: 120 1095 27
Fragments arranged by size:
1095 120 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 1011 1032 1038 1188 1242
Size: 280 731 21 6 150 54
Fragments arranged by size:
731 280 150 54 21 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 1242
Size: 43 1199
BsaI GGTCTCn'nnnn_
Cuts at: 0 906 1242
Size: 906 336
BsaBI GATnn'nnATC
Cuts at: 0 559 1242
Size: 559 683
BsaWI w'CCGG_w
Cuts at: 0 544 1242
Size: 544 698
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 621 651 776 806 1150 1180 1242
Size: 621 30 125 30 344 30 62
Fragments arranged by size:
621 344 125 62 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1035 1143 1191 1242
Size: 1035 108 48 51
Fragments arranged by size:
1035 108 51 48
BseYI C'CCAG_C
Cuts at: 0 490 1242
Size: 490 752
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1158 1242
Size: 1158 84
BsiEI CG_ry'CG
Cuts at: 0 52 122 1242
Size: 52 70 1120
Fragments arranged by size:
1120 70 52
BsiHKAI G_wGCw'C
Cuts at: 0 59 370 959 1115 1242
Size: 59 311 589 156 127
Fragments arranged by size:
589 311 156 127 59
BsmI GAATG_Cn'
Cuts at: 0 225 594 1242
Size: 225 369 648
Fragments arranged by size:
648 369 225
BsmBI CGTCTCn'nnnn_
Cuts at: 0 659 1242
Size: 659 583
Bsp1286I G_dGCh'C
Cuts at: 0 59 115 370 959 1115 1236 1242
Size: 59 56 255 589 156 121 6
Fragments arranged by size:
589 255 156 121 59 56 6
BspEI T'CCGG_A
Cuts at: 0 544 1242
Size: 544 698
BsrBI CCG'CTC
Cuts at: 0 183 1242
Size: 183 1059
BstXI CCAn_nnnn'nTGG
Cuts at: 0 490 809 1242
Size: 490 319 433
Fragments arranged by size:
490 433 319
BstYI r'GATC_y
Cuts at: 0 1090 1242
Size: 1090 152
BtgI C'CryG_G
Cuts at: 0 122 206 1242
Size: 122 84 1036
Fragments arranged by size:
1036 122 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1115 1242
Size: 1115 127
DrdI GACnn_nn'nnGTC
Cuts at: 0 1180 1242
Size: 1180 62
EaeI y'GGCC_r
Cuts at: 0 49 119 742 1242
Size: 49 70 623 500
Fragments arranged by size:
623 500 70 49
EagI C'GGCC_G
Cuts at: 0 49 119 1242
Size: 49 70 1123
Fragments arranged by size:
1123 70 49
EarI CTCTTCn'nnn_
Cuts at: 0 1041 1120 1242
Size: 1041 79 122
Fragments arranged by size:
1041 122 79
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 142 1242
Size: 142 1100
EcoICRI GAG'CTC
Cuts at: 0 57 1242
Size: 57 1185
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 979 1011 1032 1038 1188 1242
Size: 280 699 32 21 6 150 54
Fragments arranged by size:
699 280 150 54 32 21 6
EcoO109I rG'GnC_Cy
Cuts at: 0 506 1242
Size: 506 736
EcoRI G'AATT_C
Cuts at: 0 751 1242
Size: 751 491
FspI TGC'GCA
Cuts at: 0 224 242 1242
Size: 224 18 1000
Fragments arranged by size:
1000 224 18
HaeII r_GCGC'y
Cuts at: 0 267 615 1242
Size: 267 348 627
Fragments arranged by size:
627 348 267
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 220 252 307 339 776 808 1057 1069
Size: 220 32 55 32 437 32 249 12
Cuts at: 1069 1075 1089 1101 1107 1150 1182 1242
Size: 6 14 12 6 43 32 60
Fragments arranged by size:
437 249 220 60 55 43 32 32
32 32 14 12 12 6 6
HincII GTy'rAC
Cuts at: 0 990 1242
Size: 990 252
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 760 1043 1094 1242
Size: 760 283 51 148
Fragments arranged by size:
760 283 148 51
MscI TGG'CCA
Cuts at: 0 744 1242
Size: 744 498
MslI CAynn'nnrTG
Cuts at: 0 655 1242
Size: 655 587
MspA1I CmG'CkG
Cuts at: 0 159 1242
Size: 159 1083
NspI r_CATG'y
Cuts at: 0 820 1242
Size: 820 422
PasI CC'CwG_GG
Cuts at: 0 641 1242
Size: 641 601
PflMI CCAn_nnn'nTGG
Cuts at: 0 584 1242
Size: 584 658
PfoI T'CCnGG_A
Cuts at: 0 1204 1242
Size: 1204 38
PvuII CAG'CTG
Cuts at: 0 159 1242
Size: 159 1083
SacI G_AGCT'C
Cuts at: 0 59 1242
Size: 59 1183
SexAI A'CCwGG_T
Cuts at: 0 700 1242
Size: 700 542
SmlI C'TyrA_G
Cuts at: 0 58 371 1242
Size: 58 313 871
Fragments arranged by size:
871 313 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 868 1242
Size: 868 374
TatI w'GTAC_w
Cuts at: 0 356 1242
Size: 356 886
Tth111I GACn'n_nGTC
Cuts at: 0 1180 1242
Size: 1180 62
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 809 882 1242
Size: 809 73 360
Fragments arranged by size:
809 360 73
XhoI C'TCGA_G
Cuts at: 0 371 1242
Size: 371 871
Enzymes that do cut:
AcuI AfeI AlwNI ApaLI ApoI AvaI BamHI BanI
BanII BbsI BlpI Bme1580I BmgBI BmrI BplI BpmI
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BsrBI BstXI
BstYI BtgI BtgZI DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII
MmeI MscI MslI MspA1I NspI PasI PflMI PfoI
PvuII SacI SexAI SmlI TaqII TatI Tth111I XcmI
XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmtI Bpu10I BsaAI BsaHI BsiWI BspHI
BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII
EcoNI EcoRV FalI FseI FspAI HindIII HpaI KasI
KpnI MfeI MluI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI XbaI
XmaI XmnI ZraI