Restriction map for
pF1KSDA0557
(Linear) (Six-base) MAPSORT of: KIAA0557.seq Check: 1884 from: 1 to: 1440
KIAA0557.orf
With 182 enzymes: SgfI *
October 24, 2006 15:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 985 1321 1440
Size: 985 336 119
Fragments arranged by size:
985 336 119
AatII G_ACGT'C
Cuts at: 0 1012 1440
Size: 1012 428
AccI GT'mk_AC
Cuts at: 0 692 1440
Size: 692 748
Acc65I G'GTAC_C
Cuts at: 0 824 1440
Size: 824 616
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 984 1086 1152 1254 1440
Size: 984 102 66 102 186
Fragments arranged by size:
984 186 102 102 66
AfeI AGC'GCT
Cuts at: 0 1165 1249 1440
Size: 1165 84 191
Fragments arranged by size:
1165 191 84
AleI CACnn'nnGTG
Cuts at: 0 1295 1440
Size: 1295 145
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1369 1440
Size: 1369 71
AlwNI CAG_nnn'CTG
Cuts at: 0 27 48 267 294 1440
Size: 27 21 219 27 1146
Fragments arranged by size:
1146 219 27 27 21
ApaI G_GGCC'C
Cuts at: 0 191 577 1440
Size: 191 386 863
Fragments arranged by size:
863 386 191
ApaLI G'TGCA_C
Cuts at: 0 1120 1440
Size: 1120 320
AvaI C'yCGr_G
Cuts at: 0 191 323 448 1150 1440
Size: 191 132 125 702 290
Fragments arranged by size:
702 290 191 132 125
BamHI G'GATC_C
Cuts at: 0 767 1440
Size: 767 673
BanI G'GyrC_C
Cuts at: 0 441 663 824 1345 1440
Size: 441 222 161 521 95
Fragments arranged by size:
521 441 222 161 95
BanII G_rGCy'C
Cuts at: 0 110 191 418 577 1180 1440
Size: 110 81 227 159 603 260
Fragments arranged by size:
603 260 227 159 110 81
BbsI GAAGACnn'nnnn_
Cuts at: 0 948 1440
Size: 948 492
BclI T'GATC_A
Cuts at: 0 912 1440
Size: 912 528
BfrBI ATG'CAT
Cuts at: 0 719 1440
Size: 719 721
BlpI GC'TnA_GC
Cuts at: 0 488 553 731 1440
Size: 488 65 178 709
Fragments arranged by size:
709 488 178 65
Bme1580I G_kGCm'C
Cuts at: 0 191 446 577 668 1124 1150 1348 1440
Size: 191 255 131 91 456 26 198 92
Fragments arranged by size:
456 255 198 191 131 92 91 26
BmgBI CAC'GTC
Cuts at: 0 684 1440
Size: 684 756
BmrI ACTGGGnnnn_n'
Cuts at: 0 883 1230 1440
Size: 883 347 210
Fragments arranged by size:
883 347 210
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 126 617 1440
Size: 126 491 823
Fragments arranged by size:
823 491 126
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 289 304 513 529 587 769 1164
Size: 4 285 15 209 16 58 182 395
Cuts at: 1164 1387 1439 1440
Size: 223 52 1
Fragments arranged by size:
395 285 223 209 182 58 52 16
15 4 1
Bpu10I CC'TnA_GC
Cuts at: 0 202 1440
Size: 202 1238
BsaHI Gr'CG_yC
Cuts at: 0 628 1009 1440
Size: 628 381 431
Fragments arranged by size:
628 431 381
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 613 643 1440
Size: 613 30 797
Fragments arranged by size:
797 613 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 97 118 370 721 1440
Size: 97 21 252 351 719
Fragments arranged by size:
719 351 252 97 21
BseYI C'CCAG_C
Cuts at: 0 130 184 543 548 726 1440
Size: 130 54 359 5 178 714
Fragments arranged by size:
714 359 178 130 54 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1215 1339 1440
Size: 1215 124 101
Fragments arranged by size:
1215 124 101
BsiEI CG_ry'CG
Cuts at: 0 241 1092 1440
Size: 241 851 348
Fragments arranged by size:
851 348 241
BsiHKAI G_wGCw'C
Cuts at: 0 836 1124 1180 1440
Size: 836 288 56 260
Fragments arranged by size:
836 288 260 56
BsmBI CGTCTCn'nnnn_
Cuts at: 0 112 1440
Size: 112 1328
Bsp1286I G_dGCh'C
Cuts at: 0 110 191 418 446 577 668 836 1124
Size: 110 81 227 28 131 91 168 288
Cuts at: 1124 1150 1180 1348 1440
Size: 26 30 168 92
Fragments arranged by size:
288 227 168 168 131 110 92 91
81 30 28 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 985 1321 1361 1440
Size: 985 336 40 79
Fragments arranged by size:
985 336 79 40
BsrBI CCG'CTC
Cuts at: 0 350 867 1050 1094 1218 1440
Size: 350 517 183 44 124 222
Fragments arranged by size:
517 350 222 183 124 44
BsrFI r'CCGG_y
Cuts at: 0 1407 1440
Size: 1407 33
BssSI C'ACGA_G
Cuts at: 0 370 1440
Size: 370 1070
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1238 1440
Size: 1238 202
BstXI CCAn_nnnn'nTGG
Cuts at: 0 856 1440
Size: 856 584
BstYI r'GATC_y
Cuts at: 0 62 263 314 498 767 1440
Size: 62 201 51 184 269 673
Fragments arranged by size:
673 269 201 184 62 51
BstZ17I GTA'TAC
Cuts at: 0 693 1440
Size: 693 747
BtgI C'CryG_G
Cuts at: 0 113 362 954 1440
Size: 113 249 592 486
Fragments arranged by size:
592 486 249 113
DraIII CAC_nnn'GTG
Cuts at: 0 1238 1440
Size: 1238 202
EaeI y'GGCC_r
Cuts at: 0 2 238 586 858 1326 1440
Size: 2 236 348 272 468 114
Fragments arranged by size:
468 348 272 236 114 2
EagI C'GGCC_G
Cuts at: 0 238 1440
Size: 238 1202
EarI CTCTTCn'nnn_
Cuts at: 0 53 95 160 169 509 1440
Size: 53 42 65 9 340 931
Fragments arranged by size:
931 340 65 53 42 9
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 136 1327 1440
Size: 136 1191 113
Fragments arranged by size:
1191 136 113
EcoICRI GAG'CTC
Cuts at: 0 1178 1440
Size: 1178 262
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 4 289 304 513 529 587 769 984
Size: 4 285 15 209 16 58 182 215
Cuts at: 984 1086 1152 1164 1254 1387 1439 1440
Size: 102 66 12 90 133 52 1
Fragments arranged by size:
285 215 209 182 133 102 90 66
58 52 16 15 12 4 1
EcoNI CCTnn'n_nnAGG
Cuts at: 0 52 1440
Size: 52 1388
EcoO109I rG'GnC_Cy
Cuts at: 0 45 126 188 198 573 598 760 853
Size: 45 81 62 10 375 25 162 93
Cuts at: 853 1423 1440
Size: 570 17
Fragments arranged by size:
570 375 162 93 81 62 45 25
17 10
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 965 1440
Size: 965 475
FspI TGC'GCA
Cuts at: 0 250 1440
Size: 250 1190
HaeII r_GCGC'y
Cuts at: 0 1167 1251 1277 1440
Size: 1167 84 26 163
Fragments arranged by size:
1167 163 84 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 306 338 487 519 613 645 752 784
Size: 306 32 149 32 94 32 107 32
Cuts at: 784 1440
Size: 656
Fragments arranged by size:
656 306 149 107 94 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 828 1440
Size: 828 612
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 755 1121 1440
Size: 755 366 319
Fragments arranged by size:
755 366 319
MscI TGG'CCA
Cuts at: 0 4 588 860 1440
Size: 4 584 272 580
Fragments arranged by size:
584 580 272 4
MslI CAynn'nnrTG
Cuts at: 0 1295 1440
Size: 1295 145
MspA1I CmG'CkG
Cuts at: 0 230 387 411 552 579 735 747 1211
Size: 230 157 24 141 27 156 12 464
Cuts at: 1211 1368 1440
Size: 157 72
Fragments arranged by size:
464 230 157 157 156 141 72 27
24 12
NcoI C'CATG_G
Cuts at: 0 954 1440
Size: 954 486
NsiI A_TGCA'T
Cuts at: 0 721 1440
Size: 721 719
PflMI CCAn_nnn'nTGG
Cuts at: 0 180 1410 1440
Size: 180 1230 30
Fragments arranged by size:
1230 180 30
PfoI T'CCnGG_A
Cuts at: 0 266 1416 1440
Size: 266 1150 24
Fragments arranged by size:
1150 266 24
PpuMI rG'GwC_Cy
Cuts at: 0 45 126 760 1440
Size: 45 81 634 680
Fragments arranged by size:
680 634 81 45
PspOMI G'GGCC_C
Cuts at: 0 187 573 1440
Size: 187 386 867
Fragments arranged by size:
867 386 187
PstI C_TGCA'G
Cuts at: 0 386 1440
Size: 386 1054
PvuII CAG'CTG
Cuts at: 0 552 1440
Size: 552 888
SacI G_AGCT'C
Cuts at: 0 1180 1440
Size: 1180 260
SapI GCTCTTCn'nnn_
Cuts at: 0 160 169 1440
Size: 160 9 1271
Fragments arranged by size:
1271 160 9
SfcI C'TryA_G
Cuts at: 0 382 1440
Size: 382 1058
SmaI CCC'GGG
Cuts at: 0 193 1440
Size: 193 1247
StuI AGG'CCT
Cuts at: 0 909 1440
Size: 909 531
StyI C'CwwG_G
Cuts at: 0 35 254 954 1440
Size: 35 219 700 486
Fragments arranged by size:
700 486 219 35
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1249 1440
Size: 1249 191
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 181 1440
Size: 181 1259
XmaI C'CCGG_G
Cuts at: 0 191 1440
Size: 191 1249
XmnI GAAnn'nnTTC
Cuts at: 0 1165 1249 1440
Size: 1165 84 191
Fragments arranged by size:
1165 191 84
ZraI GAC'GTC
Cuts at: 0 1010 1440
Size: 1010 430
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AfeI AleI AlfI
AlwNI ApaI ApaLI AvaI BamHI BanI BanII BbsI
BclI BfrBI BlpI Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI
BstYI BstZ17I BtgI DraIII EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII Hin4I
KpnI MmeI MscI MslI MspA1I NcoI NsiI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SapI SfcI
SmaI StuI StyI TaqII XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AflII AflIII AgeI AhdI AloI ApoI AscI
AseI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BglI BglII BmtI BpuEI BsaI BsaAI BsaBI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI
BstEII Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EcoRI EcoRV FseI FspAI HincII HindIII HpaI KasI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI
SanDI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
TstI Tth111I XbaI XhoI