Restriction map for
pF1KSDA0547
(Linear) (Six-base) MAPSORT of: KIAA0547.seq Check: 215 from: 1 to: 2058
KIAA0547.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 1730 2058
Size: 1730 328
Acc65I G'GTAC_C
Cuts at: 0 1999 2058
Size: 1999 59
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 995 1084 1613 1967 2058
Size: 922 73 89 529 354 91
Fragments arranged by size:
922 529 354 91 89 73
AflII C'TTAA_G
Cuts at: 0 2043 2058
Size: 2043 15
AleI CACnn'nnGTG
Cuts at: 0 961 2058
Size: 961 1097
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 480 2058
Size: 480 1578
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1975 2007 2058
Size: 1975 32 51
Fragments arranged by size:
1975 51 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1616 1962 2058
Size: 1616 346 96
Fragments arranged by size:
1616 346 96
ApaI G_GGCC'C
Cuts at: 0 7 2058
Size: 7 2051
ApoI r'AATT_y
Cuts at: 0 817 883 2058
Size: 817 66 1175
Fragments arranged by size:
1175 817 66
AscI GG'CGCG_CC
Cuts at: 0 136 224 2058
Size: 136 88 1834
Fragments arranged by size:
1834 136 88
AseI AT'TA_AT
Cuts at: 0 1912 2058
Size: 1912 146
AvaI C'yCGr_G
Cuts at: 0 317 580 1654 2058
Size: 317 263 1074 404
Fragments arranged by size:
1074 404 317 263
BamHI G'GATC_C
Cuts at: 0 1568 2058
Size: 1568 490
BanI G'GyrC_C
Cuts at: 0 517 764 799 1999 2058
Size: 517 247 35 1200 59
Fragments arranged by size:
1200 517 247 59 35
BanII G_rGCy'C
Cuts at: 0 7 431 530 1099 1638 1873 2058
Size: 7 424 99 569 539 235 185
Fragments arranged by size:
569 539 424 235 185 99 7
BbeI G_GCGC'C
Cuts at: 0 521 768 2058
Size: 521 247 1290
Fragments arranged by size:
1290 521 247
BbsI GAAGACnn'nnnn_
Cuts at: 0 1080 2058
Size: 1080 978
BbvCI CC'TCA_GC
Cuts at: 0 64 2058
Size: 64 1994
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 685 719 2058
Size: 685 34 1339
Fragments arranged by size:
1339 685 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1945 2058
Size: 1945 113
BclI T'GATC_A
Cuts at: 0 1847 2058
Size: 1847 211
BglI GCCn_nnn'nGGC
Cuts at: 0 1214 2058
Size: 1214 844
BglII A'GATC_T
Cuts at: 0 251 2058
Size: 251 1807
Bme1580I G_kGCm'C
Cuts at: 0 7 2058
Size: 7 2051
BmgBI CAC'GTC
Cuts at: 0 351 750 2058
Size: 351 399 1308
Fragments arranged by size:
1308 399 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 1309 1567 2058
Size: 1309 258 491
Fragments arranged by size:
1309 491 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 964 2058
Size: 964 1094
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 754 1250 1324 1667 1856 2058
Size: 478 276 496 74 343 189 202
Fragments arranged by size:
496 478 343 276 202 189 74
Bpu10I CC'TnA_GC
Cuts at: 0 64 2058
Size: 64 1994
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 653 1113 1161 2058
Size: 653 460 48 897
Fragments arranged by size:
897 653 460 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1657 1689 2058
Size: 1657 32 369
Fragments arranged by size:
1657 369 32
BsaAI yAC'GTr
Cuts at: 0 96 2058
Size: 96 1962
BsaHI Gr'CG_yC
Cuts at: 0 385 518 674 765 1248 2058
Size: 385 133 156 91 483 810
Fragments arranged by size:
810 483 385 156 133 91
BsaWI w'CCGG_w
Cuts at: 0 345 2058
Size: 345 1713
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 418 448 934 964 1829 1859 2058
Size: 418 30 486 30 865 30 199
Fragments arranged by size:
865 486 418 199 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 904 1681 1815 2058
Size: 520 384 777 134 243
Fragments arranged by size:
777 520 384 243 134
BseYI C'CCAG_C
Cuts at: 0 620 2050 2058
Size: 620 1430 8
Fragments arranged by size:
1430 620 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1136 1602 2058
Size: 118 1018 466 456
Fragments arranged by size:
1018 466 456 118
BsiHKAI G_wGCw'C
Cuts at: 0 1688 1781 1873 2058
Size: 1688 93 92 185
Fragments arranged by size:
1688 185 93 92
BsmI GAATG_Cn'
Cuts at: 0 1443 1602 2058
Size: 1443 159 456
Fragments arranged by size:
1443 456 159
BsmBI CGTCTCn'nnnn_
Cuts at: 0 376 2058
Size: 376 1682
Bsp1286I G_dGCh'C
Cuts at: 0 7 431 530 1099 1638 1688 1781 1873
Size: 7 424 99 569 539 50 93 92
Cuts at: 1873 2058
Size: 185
Fragments arranged by size:
569 539 424 185 99 93 92 50
7
BspEI T'CCGG_A
Cuts at: 0 345 2058
Size: 345 1713
BsrBI CCG'CTC
Cuts at: 0 150 2058
Size: 150 1908
BsrFI r'CCGG_y
Cuts at: 0 851 2058
Size: 851 1207
BssHII G'CGCG_C
Cuts at: 0 85 136 201 224 245 2058
Size: 85 51 65 23 21 1813
Fragments arranged by size:
1813 85 65 51 23 21
BstAPI GCAn_nnn'nTGC
Cuts at: 0 198 2058
Size: 198 1860
BstYI r'GATC_y
Cuts at: 0 251 1568 1808 2058
Size: 251 1317 240 250
Fragments arranged by size:
1317 251 250 240
BtgI C'CryG_G
Cuts at: 0 302 521 596 803 1903 2058
Size: 302 219 75 207 1100 155
Fragments arranged by size:
1100 302 219 207 155 75
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 591 2058
Size: 591 1467
BtsI GCAGTG_nn'
Cuts at: 0 192 825 1146 2058
Size: 192 633 321 912
Fragments arranged by size:
912 633 321 192
DraIII CAC_nnn'GTG
Cuts at: 0 198 1430 2058
Size: 198 1232 628
Fragments arranged by size:
1232 628 198
EaeI y'GGCC_r
Cuts at: 0 80 557 681 1074 1407 1906 2058
Size: 80 477 124 393 333 499 152
Fragments arranged by size:
499 477 393 333 152 124 80
EarI CTCTTCn'nnn_
Cuts at: 0 1060 1943 2058
Size: 1060 883 115
Fragments arranged by size:
1060 883 115
EcoICRI GAG'CTC
Cuts at: 0 1871 2058
Size: 1871 187
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 754 922 995 1084 1250 1324 1613
Size: 478 276 168 73 89 166 74 289
Cuts at: 1613 1667 1856 1967 2058
Size: 54 189 111 91
Fragments arranged by size:
478 289 276 189 168 166 111 91
89 74 73 54
EcoNI CCTnn'n_nnAGG
Cuts at: 0 559 2058
Size: 559 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 4 102 510 1053 1380 2058
Size: 4 98 408 543 327 678
Fragments arranged by size:
678 543 408 327 98 4
EcoRI G'AATT_C
Cuts at: 0 817 2058
Size: 817 1241
HaeII r_GCGC'y
Cuts at: 0 62 269 521 768 2058
Size: 62 207 252 247 1290
Fragments arranged by size:
1290 252 247 207 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 243 275 418 450 523 555 651 683
Size: 243 32 143 32 73 32 96 32
Cuts at: 683 934 966 1174 1206 2058
Size: 251 32 208 32 852
Fragments arranged by size:
852 251 243 208 143 96 73 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 404 2058
Size: 404 1654
HpaI GTT'AAC
Cuts at: 0 404 2058
Size: 404 1654
KasI G'GCGC_C
Cuts at: 0 517 764 2058
Size: 517 247 1294
Fragments arranged by size:
1294 517 247
KpnI G_GTAC'C
Cuts at: 0 2003 2058
Size: 2003 55
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1782 2058
Size: 1782 276
MscI TGG'CCA
Cuts at: 0 683 1076 1908 2058
Size: 683 393 832 150
Fragments arranged by size:
832 683 393 150
MslI CAynn'nnrTG
Cuts at: 0 961 2058
Size: 961 1097
MspA1I CmG'CkG
Cuts at: 0 304 523 598 762 2058
Size: 304 219 75 164 1296
Fragments arranged by size:
1296 304 219 164 75
NaeI GCC'GGC
Cuts at: 0 853 2058
Size: 853 1205
NarI GG'CG_CC
Cuts at: 0 518 765 2058
Size: 518 247 1293
Fragments arranged by size:
1293 518 247
NcoI C'CATG_G
Cuts at: 0 1903 2058
Size: 1903 155
NdeI CA'TA_TG
Cuts at: 0 1896 2058
Size: 1896 162
NgoMIV G'CCGG_C
Cuts at: 0 851 2058
Size: 851 1207
PasI CC'CwG_GG
Cuts at: 0 513 2058
Size: 513 1545
PflMI CCAn_nnn'nTGG
Cuts at: 0 501 2058
Size: 501 1557
PfoI T'CCnGG_A
Cuts at: 0 1833 2058
Size: 1833 225
PpuMI rG'GwC_Cy
Cuts at: 0 102 1053 1380 2058
Size: 102 951 327 678
Fragments arranged by size:
951 678 327 102
PspOMI G'GGCC_C
Cuts at: 0 3 2058
Size: 3 2055
PspXI vC'TCGA_Gb
Cuts at: 0 580 2058
Size: 580 1478
PstI C_TGCA'G
Cuts at: 0 327 2058
Size: 327 1731
SacI G_AGCT'C
Cuts at: 0 1873 2058
Size: 1873 185
SacII CC_GC'GG
Cuts at: 0 305 524 599 2058
Size: 305 219 75 1459
Fragments arranged by size:
1459 305 219 75
SapI GCTCTTCn'nnn_
Cuts at: 0 1943 2058
Size: 1943 115
SfcI C'TryA_G
Cuts at: 0 323 1859 2058
Size: 323 1536 199
Fragments arranged by size:
1536 323 199
SfoI GGC'GCC
Cuts at: 0 519 766 2058
Size: 519 247 1292
Fragments arranged by size:
1292 519 247
SgrAI Cr'CCGG_yG
Cuts at: 0 851 2058
Size: 851 1207
SmaI CCC'GGG
Cuts at: 0 319 2058
Size: 319 1739
SmlI C'TyrA_G
Cuts at: 0 580 668 1092 1176 2043 2058
Size: 580 88 424 84 867 15
Fragments arranged by size:
867 580 424 88 84 15
SpeI A'CTAG_T
Cuts at: 0 1930 2058
Size: 1930 128
SrfI GCCC'GGGC
Cuts at: 0 319 2058
Size: 319 1739
SspI AAT'ATT
Cuts at: 0 867 1480 2058
Size: 867 613 578
Fragments arranged by size:
867 613 578
StuI AGG'CCT
Cuts at: 0 666 2058
Size: 666 1392
StyI C'CwwG_G
Cuts at: 0 1328 1753 1903 1968 2058
Size: 1328 425 150 65 90
Fragments arranged by size:
1328 425 150 90 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 558 2058
Size: 558 1500
TatI w'GTAC_w
Cuts at: 0 1518 2058
Size: 1518 540
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1829 1861 2058
Size: 1829 32 197
Fragments arranged by size:
1829 197 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 552 2058
Size: 552 1506
XhoI C'TCGA_G
Cuts at: 0 580 2058
Size: 580 1478
XmaI C'CCGG_G
Cuts at: 0 317 2058
Size: 317 1741
Enzymes that do cut:
AccI Acc65I AcuI AflII AleI AlfI AloI AlwNI
ApaI ApoI AscI AseI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BsrBI BsrFI BssHII BstAPI
BstYI BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI
Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I HincII HpaI
KasI KpnI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV PasI PflMI PfoI PpuMI PspOMI
PspXI PstI SacI SacII SapI SfcI SfoI SgrAI
SmaI SmlI SpeI SrfI SspI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflIII AgeI AhdI ApaLI
AsiSI AvrII BaeI BfrBI BlpI BmtI BsaBI BsiEI
BsiWI BspHI BspMI BsrDI BsrGI BssSI BstBI BstEII
BstXI BstZ17I Bsu36I ClaI CspCI DraI DrdI EagI
EciI EcoRV FalI FseI FspI FspAI HindIII MfeI
MluI NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII
RsrII SalI SanDI SbfI ScaI SexAI SfiI SgfI
SnaBI SphI SwaI Tth111I XbaI XmnI ZraI