Restriction map for
pF1KA0494
(Linear) (Six-base) MAPSORT of: KIAA0494.seq Check: 2319 from: 1 to: 1485
KIAA0494.orf
With 182 enzymes: SgfI *
April 24, 2009 13:00 ..
Acc65I G'GTAC_C
Cuts at: 0 848 1485
Size: 848 637
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 728 963 1300 1328 1485
Size: 556 172 235 337 28 157
Fragments arranged by size:
556 337 235 172 157 28
AhdI GACnn_n'nnGTC
Cuts at: 0 123 1485
Size: 123 1362
AlwNI CAG_nnn'CTG
Cuts at: 0 121 1485
Size: 121 1364
ApoI r'AATT_y
Cuts at: 0 148 322 783 1089 1228 1485
Size: 148 174 461 306 139 257
Fragments arranged by size:
461 306 257 174 148 139
AvaI C'yCGr_G
Cuts at: 0 874 1485
Size: 874 611
AvrII C'CTAG_G
Cuts at: 0 1386 1485
Size: 1386 99
BanI G'GyrC_C
Cuts at: 0 848 1485
Size: 848 637
BanII G_rGCy'C
Cuts at: 0 20 1485
Size: 20 1465
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 922 956 1485
Size: 922 34 529
Fragments arranged by size:
922 529 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1110 1485
Size: 1110 375
BclI T'GATC_A
Cuts at: 0 1127 1485
Size: 1127 358
BfrBI ATG'CAT
Cuts at: 0 25 1485
Size: 25 1460
BglII A'GATC_T
Cuts at: 0 209 997 1311 1485
Size: 209 788 314 174
Fragments arranged by size:
788 314 209 174
Bme1580I G_kGCm'C
Cuts at: 0 840 1485
Size: 840 645
BmgBI CAC'GTC
Cuts at: 0 1347 1485
Size: 1347 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 1246 1485
Size: 1246 239
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1135 1485
Size: 147 988 350
Fragments arranged by size:
988 350 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 1105 1151 1480 1485
Size: 446 659 46 329 5
Fragments arranged by size:
659 446 329 46 5
Bpu10I CC'TnA_GC
Cuts at: 0 1465 1485
Size: 1465 20
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 277 298 430 1437 1485
Size: 277 21 132 1007 48
Fragments arranged by size:
1007 277 132 48 21
BsaI GGTCTCn'nnnn_
Cuts at: 0 1165 1241 1485
Size: 1165 76 244
Fragments arranged by size:
1165 244 76
BsaBI GATnn'nnATC
Cuts at: 0 457 689 1485
Size: 457 232 796
Fragments arranged by size:
796 457 232
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 90 120 1238 1268 1485
Size: 90 30 1118 30 217
Fragments arranged by size:
1118 217 90 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 838 1485
Size: 838 647
BseYI C'CCAG_C
Cuts at: 0 40 1485
Size: 40 1445
BsiHKAI G_wGCw'C
Cuts at: 0 20 1137 1485
Size: 20 1117 348
Fragments arranged by size:
1117 348 20
Bsp1286I G_dGCh'C
Cuts at: 0 20 840 1137 1485
Size: 20 820 297 348
Fragments arranged by size:
820 348 297 20
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 273 1485
Size: 273 1212
BsrBI CCG'CTC
Cuts at: 0 222 1485
Size: 222 1263
BsrDI GCAATG_nn'
Cuts at: 0 1219 1485
Size: 1219 266
BstBI TT'CG_AA
Cuts at: 0 327 1485
Size: 327 1158
BstYI r'GATC_y
Cuts at: 0 209 300 997 1311 1485
Size: 209 91 697 314 174
Fragments arranged by size:
697 314 209 174 91
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 947 1485
Size: 947 538
BtsI GCAGTG_nn'
Cuts at: 0 506 1485
Size: 506 979
DraI TTT'AAA
Cuts at: 0 605 1485
Size: 605 880
EaeI y'GGCC_r
Cuts at: 0 1338 1485
Size: 1338 147
EarI CTCTTCn'nnn_
Cuts at: 0 131 137 571 1178 1485
Size: 131 6 434 607 307
Fragments arranged by size:
607 434 307 131 6
EcoICRI GAG'CTC
Cuts at: 0 18 1485
Size: 18 1467
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 446 556 728 963 1105 1151 1300 1328
Size: 446 110 172 235 142 46 149 28
Cuts at: 1328 1480 1485
Size: 152 5
Fragments arranged by size:
446 235 172 152 149 142 110 46
28 5
EcoRI G'AATT_C
Cuts at: 0 148 1485
Size: 148 1337
EcoRV GAT'ATC
Cuts at: 0 798 1485
Size: 798 687
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1037 1485
Size: 1037 448
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 103 109 135 141 201 233 1119 1151
Size: 103 6 26 6 60 32 886 32
Cuts at: 1151 1485
Size: 334
Fragments arranged by size:
886 334 103 60 32 32 26 6
6
HincII GTy'rAC
Cuts at: 0 491 918 1485
Size: 491 427 567
Fragments arranged by size:
567 491 427
KpnI G_GTAC'C
Cuts at: 0 852 1485
Size: 852 633
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 141 1485
Size: 141 1344
MscI TGG'CCA
Cuts at: 0 1340 1485
Size: 1340 145
MslI CAynn'nnrTG
Cuts at: 0 466 1485
Size: 466 1019
MspA1I CmG'CkG
Cuts at: 0 532 1125 1485
Size: 532 593 360
Fragments arranged by size:
593 532 360
NsiI A_TGCA'T
Cuts at: 0 27 1485
Size: 27 1458
PflMI CCAn_nnn'nTGG
Cuts at: 0 1347 1485
Size: 1347 138
PfoI T'CCnGG_A
Cuts at: 0 714 1457 1485
Size: 714 743 28
Fragments arranged by size:
743 714 28
PspXI vC'TCGA_Gb
Cuts at: 0 874 1485
Size: 874 611
PstI C_TGCA'G
Cuts at: 0 501 537 680 1485
Size: 501 36 143 805
Fragments arranged by size:
805 501 143 36
PvuII CAG'CTG
Cuts at: 0 532 1125 1485
Size: 532 593 360
Fragments arranged by size:
593 532 360
SacI G_AGCT'C
Cuts at: 0 20 1485
Size: 20 1465
SapI GCTCTTCn'nnn_
Cuts at: 0 1178 1485
Size: 1178 307
SfcI C'TryA_G
Cuts at: 0 497 533 560 634 676 1485
Size: 497 36 27 74 42 809
Fragments arranged by size:
809 497 74 42 36 27
SmlI C'TyrA_G
Cuts at: 0 292 313 409 874 1416 1485
Size: 292 21 96 465 542 69
Fragments arranged by size:
542 465 292 96 69 21
StuI AGG'CCT
Cuts at: 0 1164 1485
Size: 1164 321
StyI C'CwwG_G
Cuts at: 0 182 1386 1485
Size: 182 1204 99
Fragments arranged by size:
1204 182 99
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 979 1485
Size: 979 506
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 90 122 1485
Size: 90 32 1363
Fragments arranged by size:
1363 90 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1348 1485
Size: 1348 137
XhoI C'TCGA_G
Cuts at: 0 874 1485
Size: 874 611
XmnI GAAnn'nnTTC
Cuts at: 0 323 1313 1485
Size: 323 990 172
Fragments arranged by size:
990 323 172
Enzymes that do cut:
Acc65I AcuI AhdI AlwNI ApoI AvaI AvrII BanI
BanII BcgI BciVI BclI BfrBI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaXI
BseRI BseYI BsiHKAI Bsp1286I BspMI BsrBI BsrDI BstBI
BstYI BtgZI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoRI EcoRV FalI Hin4I HincII KpnI MmeI MscI
MslI MspA1I NsiI PflMI PfoI PspXI PstI PvuII
SacI SapI SfcI SmlI StuI StyI TaqII TstI
XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BbvCI BglI BlpI BmtI
BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrFI BsrGI BssHII BssSI BstAPI BstEII
BstXI BstZ17I Bsu36I BtgI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoO109I FseI FspI FspAI HaeII
HindIII HpaI KasI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TatI Tth111I XbaI XmaI
ZraI