Restriction map for
pF1KSDA0475
(Linear) (Six-base) MAPSORT of: KIAA0475.seq Check: 1906 from: 1 to: 1227
KIAA0475.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1227
Size: 254 973
ApaLI G'TGCA_C
Cuts at: 0 1164 1227
Size: 1164 63
AvaI C'yCGr_G
Cuts at: 0 218 1227
Size: 218 1009
BanI G'GyrC_C
Cuts at: 0 156 1227
Size: 156 1071
BanII G_rGCy'C
Cuts at: 0 486 1227
Size: 486 741
BbsI GAAGACnn'nnnn_
Cuts at: 0 1208 1227
Size: 1208 19
BfrBI ATG'CAT
Cuts at: 0 264 1227
Size: 264 963
Bme1580I G_kGCm'C
Cuts at: 0 1168 1227
Size: 1168 59
BmrI ACTGGGnnnn_n'
Cuts at: 0 198 1087 1227
Size: 198 889 140
Fragments arranged by size:
889 198 140
Bpu10I CC'TnA_GC
Cuts at: 0 371 1227
Size: 371 856
BsaI GGTCTCn'nnnn_
Cuts at: 0 380 1227
Size: 380 847
BsaBI GATnn'nnATC
Cuts at: 0 1109 1227
Size: 1109 118
BsaHI Gr'CG_yC
Cuts at: 0 1118 1227
Size: 1118 109
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1072 1102 1227
Size: 1072 30 125
Fragments arranged by size:
1072 125 30
BseYI C'CCAG_C
Cuts at: 0 249 1227
Size: 249 978
BsiHKAI G_wGCw'C
Cuts at: 0 559 1100 1168 1227
Size: 559 541 68 59
Fragments arranged by size:
559 541 68 59
BsmI GAATG_Cn'
Cuts at: 0 968 1227
Size: 968 259
Bsp1286I G_dGCh'C
Cuts at: 0 486 559 1100 1168 1227
Size: 486 73 541 68 59
Fragments arranged by size:
541 486 73 68 59
BsrDI GCAATG_nn'
Cuts at: 0 864 1227
Size: 864 363
BstXI CCAn_nnnn'nTGG
Cuts at: 0 683 1227
Size: 683 544
BstYI r'GATC_y
Cuts at: 0 655 1227
Size: 655 572
BtgI C'CryG_G
Cuts at: 0 269 709 1070 1121 1227
Size: 269 440 361 51 106
Fragments arranged by size:
440 361 269 106 51
BtsI GCAGTG_nn'
Cuts at: 0 1168 1227
Size: 1168 59
DrdI GACnn_nn'nnGTC
Cuts at: 0 835 1227
Size: 835 392
EaeI y'GGCC_r
Cuts at: 0 272 740 1148 1227
Size: 272 468 408 79
Fragments arranged by size:
468 408 272 79
EarI CTCTTCn'nnn_
Cuts at: 0 230 1227
Size: 230 997
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 254 1227
Size: 254 973
EcoO109I rG'GnC_Cy
Cuts at: 0 116 1227
Size: 116 1111
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1074 1227
Size: 1074 153
HaeII r_GCGC'y
Cuts at: 0 905 1227
Size: 905 322
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1072 1104 1227
Size: 1072 32 123
Fragments arranged by size:
1072 123 32
HindIII A'AGCT_T
Cuts at: 0 936 1227
Size: 936 291
MscI TGG'CCA
Cuts at: 0 274 742 1150 1227
Size: 274 468 408 77
Fragments arranged by size:
468 408 274 77
MslI CAynn'nnrTG
Cuts at: 0 875 1003 1227
Size: 875 128 224
Fragments arranged by size:
875 224 128
MspA1I CmG'CkG
Cuts at: 0 94 321 549 835 1131 1227
Size: 94 227 228 286 296 96
Fragments arranged by size:
296 286 228 227 96 94
NcoI C'CATG_G
Cuts at: 0 269 709 1070 1227
Size: 269 440 361 157
Fragments arranged by size:
440 361 269 157
NsiI A_TGCA'T
Cuts at: 0 266 1227
Size: 266 961
NspI r_CATG'y
Cuts at: 0 268 1227
Size: 268 959
PasI CC'CwG_GG
Cuts at: 0 188 1227
Size: 188 1039
PflMI CCAn_nnn'nTGG
Cuts at: 0 210 396 1227
Size: 210 186 831
Fragments arranged by size:
831 210 186
PsiI TTA'TAA
Cuts at: 0 308 1227
Size: 308 919
PstI C_TGCA'G
Cuts at: 0 695 1227
Size: 695 532
PvuII CAG'CTG
Cuts at: 0 94 321 549 835 1227
Size: 94 227 228 286 392
Fragments arranged by size:
392 286 228 227 94
ScaI AGT'ACT
Cuts at: 0 1193 1227
Size: 1193 34
SfcI C'TryA_G
Cuts at: 0 569 691 1227
Size: 569 122 536
Fragments arranged by size:
569 536 122
SmaI CCC'GGG
Cuts at: 0 220 1227
Size: 220 1007
SphI G_CATG'C
Cuts at: 0 268 1227
Size: 268 959
StyI C'CwwG_G
Cuts at: 0 269 488 709 1070 1227
Size: 269 219 221 361 157
Fragments arranged by size:
361 269 221 219 157
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 174 198 722 1227
Size: 174 24 524 505
Fragments arranged by size:
524 505 174 24
TatI w'GTAC_w
Cuts at: 0 1191 1227
Size: 1191 36
XmaI C'CCGG_G
Cuts at: 0 218 1227
Size: 218 1009
Enzymes that do cut:
AcuI ApaLI AvaI BanI BanII BbsI BfrBI Bme1580I
BmrI Bpu10I BsaI BsaBI BsaHI BsaXI BseYI BsiHKAI
BsmI Bsp1286I BsrDI BstXI BstYI BtgI BtsI DrdI
EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HindIII
MscI MslI MspA1I NcoI NsiI NspI PasI PflMI
PsiI PstI PvuII ScaI SfcI SmaI SphI StyI
TaqII TatI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BclI BglI BglII BlpI BmgBI BmtI
BplI BpmI BpuEI BsaAI BsaWI BseRI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI
ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MmeI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TstI Tth111I XbaI XcmI XhoI XmnI
ZraI