Restriction map for
pF1KSDA0470
(Linear) (Six-base) MAPSORT of: KIAA0470.seq Check: 4139 from: 1 to: 4380
KIAA0470.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AccI GT'mk_AC
Cuts at: 0 151 1763 2765 4323 4380
Size: 151 1612 1002 1558 57
Fragments arranged by size:
1612 1558 1002 151 57
AclI AA'CG_TT
Cuts at: 0 1449 4380
Size: 1449 2931
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 340 359 464 485 620 1720 2633
Size: 338 2 19 105 21 135 1100 913
Cuts at: 2633 3023 3104 3227 3362 4380
Size: 390 81 123 135 1018
Fragments arranged by size:
1100 1018 913 390 338 135 135 123
105 81 21 19 2
AfeI AGC'GCT
Cuts at: 0 3408 4380
Size: 3408 972
AflII C'TTAA_G
Cuts at: 0 328 1197 4380
Size: 328 869 3183
Fragments arranged by size:
3183 869 328
AleI CACnn'nnGTG
Cuts at: 0 2698 4380
Size: 2698 1682
AlwNI CAG_nnn'CTG
Cuts at: 0 436 1166 2503 3589 4065 4380
Size: 436 730 1337 1086 476 315
Fragments arranged by size:
1337 1086 730 476 436 315
ApoI r'AATT_y
Cuts at: 0 739 847 1519 2076 2147 2725 3292 4288
Size: 739 108 672 557 71 578 567 996
Cuts at: 4288 4380
Size: 92
Fragments arranged by size:
996 739 672 578 567 557 108 92
71
AvaI C'yCGr_G
Cuts at: 0 3536 4215 4380
Size: 3536 679 165
Fragments arranged by size:
3536 679 165
BamHI G'GATC_C
Cuts at: 0 2352 4380
Size: 2352 2028
BanI G'GyrC_C
Cuts at: 0 981 4380
Size: 981 3399
BanII G_rGCy'C
Cuts at: 0 95 1158 1353 4257 4380
Size: 95 1063 195 2904 123
Fragments arranged by size:
2904 1063 195 123 95
BbsI GAAGACnn'nnnn_
Cuts at: 0 926 1991 3470 4380
Size: 926 1065 1479 910
Fragments arranged by size:
1479 1065 926 910
BbvCI CC'TCA_GC
Cuts at: 0 2141 2880 3360 4380
Size: 2141 739 480 1020
Fragments arranged by size:
2141 1020 739 480
BciVI GTATCCnnnnn_n'
Cuts at: 0 3099 4380
Size: 3099 1281
BclI T'GATC_A
Cuts at: 0 909 1497 2832 4380
Size: 909 588 1335 1548
Fragments arranged by size:
1548 1335 909 588
BfrBI ATG'CAT
Cuts at: 0 3681 4380
Size: 3681 699
BglII A'GATC_T
Cuts at: 0 1332 3519 3663 4380
Size: 1332 2187 144 717
Fragments arranged by size:
2187 1332 717 144
BlpI GC'TnA_GC
Cuts at: 0 3158 4380
Size: 3158 1222
BmrI ACTGGGnnnn_n'
Cuts at: 0 2462 4148 4380
Size: 2462 1686 232
Fragments arranged by size:
2462 1686 232
BmtI G_CTAG'C
Cuts at: 0 1012 1613 4380
Size: 1012 601 2767
Fragments arranged by size:
2767 1012 601
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1652 1849 4380
Size: 1652 197 2531
Fragments arranged by size:
2531 1652 197
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2051 2561 3864 4380
Size: 2051 510 1303 516
Fragments arranged by size:
2051 1303 516 510
Bpu10I CC'TnA_GC
Cuts at: 0 2141 2880 3360 4380
Size: 2141 739 480 1020
Fragments arranged by size:
2141 1020 739 480
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 932 1207 1690 1818 3779 4017 4380
Size: 932 275 483 128 1961 238 363
Fragments arranged by size:
1961 932 483 363 275 238 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 878 4380
Size: 878 3502
BsaAI yAC'GTr
Cuts at: 0 2431 4380
Size: 2431 1949
BsaBI GATnn'nnATC
Cuts at: 0 278 1289 4380
Size: 278 1011 3091
Fragments arranged by size:
3091 1011 278
BsaHI Gr'CG_yC
Cuts at: 0 1203 4380
Size: 1203 3177
BsaWI w'CCGG_w
Cuts at: 0 219 2943 3446 4380
Size: 219 2724 503 934
Fragments arranged by size:
2724 934 503 219
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1963 1993 2093 2123 2601 2631 4380
Size: 1963 30 100 30 478 30 1749
Fragments arranged by size:
1963 1749 478 100 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1016 1984 2189 4380
Size: 1016 968 205 2191
Fragments arranged by size:
2191 1016 968 205
BseYI C'CCAG_C
Cuts at: 0 957 1150 1643 2236 4380
Size: 957 193 493 593 2144
Fragments arranged by size:
2144 957 593 493 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 654 842 1511 4380
Size: 469 185 188 669 2869
Fragments arranged by size:
2869 669 469 188 185
BsiEI CG_ry'CG
Cuts at: 0 3774 4380
Size: 3774 606
BsiHKAI G_wGCw'C
Cuts at: 0 95 3024 4380
Size: 95 2929 1356
Fragments arranged by size:
2929 1356 95
BsmI GAATG_Cn'
Cuts at: 0 4302 4380
Size: 4302 78
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1623 4380
Size: 1623 2757
Bsp1286I G_dGCh'C
Cuts at: 0 95 1158 1353 3024 4257 4380
Size: 95 1063 195 1671 1233 123
Fragments arranged by size:
1671 1233 1063 195 123 95
BspEI T'CCGG_A
Cuts at: 0 219 4380
Size: 219 4161
BspHI T'CATG_A
Cuts at: 0 777 4380
Size: 777 3603
BsrDI GCAATG_nn'
Cuts at: 0 3425 3530 4380
Size: 3425 105 850
Fragments arranged by size:
3425 850 105
BsrGI T'GTAC_A
Cuts at: 0 2895 4380
Size: 2895 1485
BssSI C'ACGA_G
Cuts at: 0 56 4380
Size: 56 4324
BstAPI GCAn_nnn'nTGC
Cuts at: 0 436 829 3076 4099 4380
Size: 436 393 2247 1023 281
Fragments arranged by size:
2247 1023 436 393 281
BstXI CCAn_nnnn'nTGG
Cuts at: 0 619 2700 4380
Size: 619 2081 1680
Fragments arranged by size:
2081 1680 619
BstYI r'GATC_y
Cuts at: 0 1332 2352 3519 3663 4380
Size: 1332 1020 1167 144 717
Fragments arranged by size:
1332 1167 1020 717 144
BstZ17I GTA'TAC
Cuts at: 0 1764 4324 4380
Size: 1764 2560 56
Fragments arranged by size:
2560 1764 56
BtgI C'CryG_G
Cuts at: 0 491 513 4380
Size: 491 22 3867
Fragments arranged by size:
3867 491 22
BtsI GCAGTG_nn'
Cuts at: 0 36 3587 3611 4380
Size: 36 3551 24 769
Fragments arranged by size:
3551 769 36 24
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1824 1859 4380
Size: 1824 35 2521
Fragments arranged by size:
2521 1824 35
DraI TTT'AAA
Cuts at: 0 2049 3957 4380
Size: 2049 1908 423
Fragments arranged by size:
2049 1908 423
DraIII CAC_nnn'GTG
Cuts at: 0 2119 4380
Size: 2119 2261
DrdI GACnn_nn'nnGTC
Cuts at: 0 3343 4380
Size: 3343 1037
EaeI y'GGCC_r
Cuts at: 0 4236 4380
Size: 4236 144
EarI CTCTTCn'nnn_
Cuts at: 0 72 1046 1538 1643 3240 3491 3647 4262
Size: 72 974 492 105 1597 251 156 615
Cuts at: 4262 4349 4380
Size: 87 31
Fragments arranged by size:
1597 974 615 492 251 156 105 87
72 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3849 4380
Size: 3849 531
EcoICRI GAG'CTC
Cuts at: 0 93 4380
Size: 93 4287
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 338 340 359 464 485 620 1720 2051
Size: 338 2 19 105 21 135 1100 331
Cuts at: 2051 2561 2633 3023 3104 3227 3362 3864 4380
Size: 510 72 390 81 123 135 502 516
Fragments arranged by size:
1100 516 510 502 390 338 331 135
135 123 105 81 72 21 19 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3119 3237 3846 4380
Size: 3119 118 609 534
Fragments arranged by size:
3119 609 534 118
EcoO109I rG'GnC_Cy
Cuts at: 0 312 4380
Size: 312 4068
EcoRI G'AATT_C
Cuts at: 0 739 4380
Size: 739 3641
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 343 706 2363 4380
Size: 343 363 1657 2017
Fragments arranged by size:
2017 1657 363 343
FspI TGC'GCA
Cuts at: 0 3016 4380
Size: 3016 1364
HaeII r_GCGC'y
Cuts at: 0 918 1167 3410 4380
Size: 918 249 2243 970
Fragments arranged by size:
2243 970 918 249
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 649 681 2773 2805 2809 2841 3055 3087
Size: 649 32 2092 32 4 32 214 32
Cuts at: 3087 3348 3380 3474 3506 4380
Size: 261 32 94 32 874
Fragments arranged by size:
2092 874 649 261 214 94 32 32
32 32 32 32 4
HincII GTy'rAC
Cuts at: 0 2561 4380
Size: 2561 1819
HindIII A'AGCT_T
Cuts at: 0 3035 4380
Size: 3035 1345
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 54 4380
Size: 54 4326
MscI TGG'CCA
Cuts at: 0 4238 4380
Size: 4238 142
MslI CAynn'nnrTG
Cuts at: 0 630 1121 1576 2698 4380
Size: 630 491 455 1122 1682
Fragments arranged by size:
1682 1122 630 491 455
MspA1I CmG'CkG
Cuts at: 0 673 2884 3934 4086 4285 4380
Size: 673 2211 1050 152 199 95
Fragments arranged by size:
2211 1050 673 199 152 95
NcoI C'CATG_G
Cuts at: 0 491 4380
Size: 491 3889
NheI G'CTAG_C
Cuts at: 0 1008 1609 4380
Size: 1008 601 2771
Fragments arranged by size:
2771 1008 601
NsiI A_TGCA'T
Cuts at: 0 3683 4380
Size: 3683 697
NspI r_CATG'y
Cuts at: 0 832 2896 4380
Size: 832 2064 1484
Fragments arranged by size:
2064 1484 832
PasI CC'CwG_GG
Cuts at: 0 865 4380
Size: 865 3515
PflMI CCAn_nnn'nTGG
Cuts at: 0 529 4234 4380
Size: 529 3705 146
Fragments arranged by size:
3705 529 146
PfoI T'CCnGG_A
Cuts at: 0 939 1884 4380
Size: 939 945 2496
Fragments arranged by size:
2496 945 939
PpuMI rG'GwC_Cy
Cuts at: 0 312 4380
Size: 312 4068
PsiI TTA'TAA
Cuts at: 0 1301 2474 4380
Size: 1301 1173 1906
Fragments arranged by size:
1906 1301 1173
PstI C_TGCA'G
Cuts at: 0 672 1368 4380
Size: 672 696 3012
Fragments arranged by size:
3012 696 672
PvuII CAG'CTG
Cuts at: 0 673 2884 3934 4086 4285 4380
Size: 673 2211 1050 152 199 95
Fragments arranged by size:
2211 1050 673 199 152 95
SacI G_AGCT'C
Cuts at: 0 95 4380
Size: 95 4285
SanDI GG'GwC_CC
Cuts at: 0 312 4380
Size: 312 4068
SapI GCTCTTCn'nnn_
Cuts at: 0 4262 4380
Size: 4262 118
ScaI AGT'ACT
Cuts at: 0 689 1587 4380
Size: 689 898 2793
Fragments arranged by size:
2793 898 689
SfcI C'TryA_G
Cuts at: 0 290 668 702 1167 1228 1364 2225 3920
Size: 290 378 34 465 61 136 861 1695
Cuts at: 3920 4380
Size: 460
Fragments arranged by size:
1695 861 465 460 378 290 136 61
34
SmaI CCC'GGG
Cuts at: 0 4217 4380
Size: 4217 163
SmlI C'TyrA_G
Cuts at: 0 328 947 1186 1197 1669 1797 3794 4032
Size: 328 619 239 11 472 128 1997 238
Cuts at: 4032 4380
Size: 348
Fragments arranged by size:
1997 619 472 348 328 239 238 128
11
SpeI A'CTAG_T
Cuts at: 0 3276 3427 4380
Size: 3276 151 953
Fragments arranged by size:
3276 953 151
SphI G_CATG'C
Cuts at: 0 832 4380
Size: 832 3548
SspI AAT'ATT
Cuts at: 0 3950 4380
Size: 3950 430
StuI AGG'CCT
Cuts at: 0 1308 4380
Size: 1308 3072
StyI C'CwwG_G
Cuts at: 0 491 1604 2983 3341 4380
Size: 491 1113 1379 358 1039
Fragments arranged by size:
1379 1113 1039 491 358
TatI w'GTAC_w
Cuts at: 0 294 687 1088 1585 2521 2895 4371 4380
Size: 294 393 401 497 936 374 1476 9
Fragments arranged by size:
1476 936 497 401 393 374 294 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1963 1995 2093 2125 2601 2633 4380
Size: 1963 32 98 32 476 32 1747
Fragments arranged by size:
1963 1747 476 98 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2415 3220 4380
Size: 2415 805 1160
Fragments arranged by size:
2415 1160 805
XmaI C'CCGG_G
Cuts at: 0 4215 4380
Size: 4215 165
XmnI GAAnn'nnTTC
Cuts at: 0 2261 4380
Size: 2261 2119
Enzymes that do cut:
AccI AclI AcuI AfeI AflII AleI AlwNI ApoI
AvaI BamHI BanI BanII BbsI BbvCI BciVI BclI
BfrBI BglII BlpI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BsrDI BsrGI BssSI BstAPI BstXI BstYI BstZ17I
BtgI BtsI CspCI DraI DraIII DrdI EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI
HaeII Hin4I HincII HindIII MmeI MscI MslI MspA1I
NcoI NheI NsiI NspI PasI PflMI PfoI PpuMI
PsiI PstI PvuII SacI SanDI SapI ScaI SfcI
SmaI SmlI SpeI SphI SspI StuI StyI TatI
TstI XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AflIII AgeI AhdI AlfI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BbeI
BcgI BglI Bme1580I BmgBI BsiWI BspMI BsrBI BsrFI
BssHII BstBI BstEII Bsu36I BtgZI ClaI EagI EcoRV
FseI FspAI HpaI KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NotI NruI PacI PciI PmeI
PmlI PpiI PshAI PspOMI PspXI PsrI PvuI RsrII
SacII SalI SbfI SexAI SfiI SfoI SgfI SgrAI
SnaBI SrfI SwaI TaqII Tth111I XcmI XhoI ZraI