Restriction map for
pF1KSDA0457
(Linear) (Six-base) MAPSORT of: KIAA0457.seq Check: 5401 from: 1 to: 2496
KIAA0457.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 96 2496
Size: 96 2400
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1054 1063 1360 2496
Size: 780 274 9 297 1136
Fragments arranged by size:
1136 780 297 274 9
AflII C'TTAA_G
Cuts at: 0 1056 2023 2496
Size: 1056 967 473
Fragments arranged by size:
1056 967 473
AflIII A'CryG_T
Cuts at: 0 1346 2496
Size: 1346 1150
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 397 455 699 1582 2478 2496
Size: 397 58 244 883 896 18
Fragments arranged by size:
896 883 397 244 58 18
AlwNI CAG_nnn'CTG
Cuts at: 0 154 2496
Size: 154 2342
ApoI r'AATT_y
Cuts at: 0 1705 2496
Size: 1705 791
AvaI C'yCGr_G
Cuts at: 0 267 2496
Size: 267 2229
BamHI G'GATC_C
Cuts at: 0 915 2496
Size: 915 1581
BanI G'GyrC_C
Cuts at: 0 22 373 2496
Size: 22 351 2123
Fragments arranged by size:
2123 351 22
BanII G_rGCy'C
Cuts at: 0 292 724 2378 2496
Size: 292 432 1654 118
Fragments arranged by size:
1654 432 292 118
BbeI G_GCGC'C
Cuts at: 0 26 2496
Size: 26 2470
BbsI GAAGACnn'nnnn_
Cuts at: 0 1148 2233 2288 2496
Size: 1148 1085 55 208
Fragments arranged by size:
1148 1085 208 55
BbvCI CC'TCA_GC
Cuts at: 0 1584 1873 2496
Size: 1584 289 623
Fragments arranged by size:
1584 623 289
BfrBI ATG'CAT
Cuts at: 0 873 1779 2496
Size: 873 906 717
Fragments arranged by size:
906 873 717
BglI GCCn_nnn'nGGC
Cuts at: 0 390 2496
Size: 390 2106
BglII A'GATC_T
Cuts at: 0 2349 2496
Size: 2349 147
BlpI GC'TnA_GC
Cuts at: 0 394 2496
Size: 394 2102
Bme1580I G_kGCm'C
Cuts at: 0 187 2496
Size: 187 2309
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 837 888 1474 1621 1882 2104 2362 2404
Size: 837 51 586 147 261 222 258 42
Cuts at: 2404 2496
Size: 92
Fragments arranged by size:
837 586 261 258 222 147 92 51
42
Bpu10I CC'TnA_GC
Cuts at: 0 957 988 1331 1584 1873 2496
Size: 957 31 343 253 289 623
Fragments arranged by size:
957 623 343 289 253 31
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 606 2496
Size: 606 1890
BsaI GGTCTCn'nnnn_
Cuts at: 0 454 822 2496
Size: 454 368 1674
Fragments arranged by size:
1674 454 368
BsaAI yAC'GTr
Cuts at: 0 1347 2496
Size: 1347 1149
BsaBI GATnn'nnATC
Cuts at: 0 2348 2496
Size: 2348 148
BsaHI Gr'CG_yC
Cuts at: 0 23 470 2496
Size: 23 447 2026
Fragments arranged by size:
2026 447 23
BsaWI w'CCGG_w
Cuts at: 0 194 284 1031 1617 2496
Size: 194 90 747 586 879
Fragments arranged by size:
879 747 586 194 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 883 913 2496
Size: 883 30 1583
Fragments arranged by size:
1583 883 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 235 891 1486 1840 1861 2177 2496
Size: 235 656 595 354 21 316 319
Fragments arranged by size:
656 595 354 319 316 235 21
BseYI C'CCAG_C
Cuts at: 0 27 174 421 476 1914 2496
Size: 27 147 247 55 1438 582
Fragments arranged by size:
1438 582 247 147 55 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 326 1153 2058 2496
Size: 326 827 905 438
Fragments arranged by size:
905 827 438 326
BsiHKAI G_wGCw'C
Cuts at: 0 1964 2378 2496
Size: 1964 414 118
Fragments arranged by size:
1964 414 118
BsmI GAATG_Cn'
Cuts at: 0 873 2496
Size: 873 1623
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1114 1353 2496
Size: 1114 239 1143
Fragments arranged by size:
1143 1114 239
Bsp1286I G_dGCh'C
Cuts at: 0 187 292 724 1964 2378 2496
Size: 187 105 432 1240 414 118
Fragments arranged by size:
1240 432 414 187 118 105
BspEI T'CCGG_A
Cuts at: 0 284 2496
Size: 284 2212
BspHI T'CATG_A
Cuts at: 0 2340 2496
Size: 2340 156
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 96 2496
Size: 96 2400
BsrDI GCAATG_nn'
Cuts at: 0 711 2496
Size: 711 1785
BsrFI r'CCGG_y
Cuts at: 0 37 1588 2496
Size: 37 1551 908
Fragments arranged by size:
1551 908 37
BssSI C'ACGA_G
Cuts at: 0 766 1356 2496
Size: 766 590 1140
Fragments arranged by size:
1140 766 590
BstAPI GCAn_nnn'nTGC
Cuts at: 0 557 2496
Size: 557 1939
BstEII G'GTnAC_C
Cuts at: 0 1216 2496
Size: 1216 1280
BstXI CCAn_nnnn'nTGG
Cuts at: 0 912 2496
Size: 912 1584
BstYI r'GATC_y
Cuts at: 0 915 2349 2496
Size: 915 1434 147
Fragments arranged by size:
1434 915 147
Bsu36I CC'TnA_GG
Cuts at: 0 17 578 1720 2496
Size: 17 561 1142 776
Fragments arranged by size:
1142 776 561 17
BtgI C'CryG_G
Cuts at: 0 446 1251 2496
Size: 446 805 1245
Fragments arranged by size:
1245 805 446
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 509 792 2496
Size: 509 283 1704
Fragments arranged by size:
1704 509 283
BtsI GCAGTG_nn'
Cuts at: 0 185 1325 2053 2496
Size: 185 1140 728 443
Fragments arranged by size:
1140 728 443 185
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1474 1509 2496
Size: 1474 35 987
Fragments arranged by size:
1474 987 35
EaeI y'GGCC_r
Cuts at: 0 400 2496
Size: 400 2096
EarI CTCTTCn'nnn_
Cuts at: 0 1944 2496
Size: 1944 552
EcoICRI GAG'CTC
Cuts at: 0 2376 2496
Size: 2376 120
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 837 888 1054 1063 1360 1474 1621
Size: 780 57 51 166 9 297 114 147
Cuts at: 1621 1882 2104 2362 2404 2496
Size: 261 222 258 42 92
Fragments arranged by size:
780 297 261 258 222 166 147 114
92 57 51 42 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1822 2496
Size: 1822 674
EcoO109I rG'GnC_Cy
Cuts at: 0 13 151 582 771 2496
Size: 13 138 431 189 1725
Fragments arranged by size:
1725 431 189 138 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2335 2496
Size: 2335 161
HaeII r_GCGC'y
Cuts at: 0 26 2496
Size: 26 2470
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 691 723 883 915 955 987 1036 1068
Size: 691 32 160 32 40 32 49 32
Cuts at: 1068 1819 1851 2087 2119 2496
Size: 751 32 236 32 377
Fragments arranged by size:
751 691 377 236 160 49 40 32
32 32 32 32 32
HincII GTy'rAC
Cuts at: 0 1734 2496
Size: 1734 762
HindIII A'AGCT_T
Cuts at: 0 624 2087 2496
Size: 624 1463 409
Fragments arranged by size:
1463 624 409
HpaI GTT'AAC
Cuts at: 0 1734 2496
Size: 1734 762
KasI G'GCGC_C
Cuts at: 0 22 2496
Size: 22 2474
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1298 2496
Size: 1298 1198
MslI CAynn'nnrTG
Cuts at: 0 2339 2496
Size: 2339 157
MspA1I CmG'CkG
Cuts at: 0 476 557 953 1274 1455 1530 1545 2042
Size: 476 81 396 321 181 75 15 497
Cuts at: 2042 2473 2496
Size: 431 23
Fragments arranged by size:
497 476 431 396 321 181 81 75
23 15
NaeI GCC'GGC
Cuts at: 0 39 2496
Size: 39 2457
NarI GG'CG_CC
Cuts at: 0 23 2496
Size: 23 2473
NcoI C'CATG_G
Cuts at: 0 446 2496
Size: 446 2050
NgoMIV G'CCGG_C
Cuts at: 0 37 2496
Size: 37 2459
NsiI A_TGCA'T
Cuts at: 0 875 1781 2496
Size: 875 906 715
Fragments arranged by size:
906 875 715
NspI r_CATG'y
Cuts at: 0 873 1783 2496
Size: 873 910 713
Fragments arranged by size:
910 873 713
PasI CC'CwG_GG
Cuts at: 0 412 1536 2496
Size: 412 1124 960
Fragments arranged by size:
1124 960 412
PflMI CCAn_nnn'nTGG
Cuts at: 0 732 2059 2496
Size: 732 1327 437
Fragments arranged by size:
1327 732 437
PfoI T'CCnGG_A
Cuts at: 0 1637 1897 2120 2496
Size: 1637 260 223 376
Fragments arranged by size:
1637 376 260 223
PmlI CAC'GTG
Cuts at: 0 1347 2496
Size: 1347 1149
PpuMI rG'GwC_Cy
Cuts at: 0 13 582 771 2496
Size: 13 569 189 1725
Fragments arranged by size:
1725 569 189 13
PstI C_TGCA'G
Cuts at: 0 93 493 562 825 1550 1714 2262 2402
Size: 93 400 69 263 725 164 548 140
Cuts at: 2402 2417 2457 2496
Size: 15 40 39
Fragments arranged by size:
725 548 400 263 164 140 93 69
40 39 15
PvuII CAG'CTG
Cuts at: 0 476 557 1455 1530 1545 2042 2473 2496
Size: 476 81 898 75 15 497 431 23
Fragments arranged by size:
898 497 476 431 81 75 23 15
SacI G_AGCT'C
Cuts at: 0 2378 2496
Size: 2378 118
SfcI C'TryA_G
Cuts at: 0 89 489 558 821 1393 1546 1710 2113
Size: 89 400 69 263 572 153 164 403
Cuts at: 2113 2144 2258 2398 2413 2453 2496
Size: 31 114 140 15 40 43
Fragments arranged by size:
572 403 400 263 164 153 140 114
89 69 43 40 31 15
SfoI GGC'GCC
Cuts at: 0 24 2496
Size: 24 2472
SgrAI Cr'CCGG_yG
Cuts at: 0 37 2496
Size: 37 2459
SmlI C'TyrA_G
Cuts at: 0 267 621 1056 2023 2496
Size: 267 354 435 967 473
Fragments arranged by size:
967 473 435 354 267
SphI G_CATG'C
Cuts at: 0 1783 2496
Size: 1783 713
StuI AGG'CCT
Cuts at: 0 275 1564 2496
Size: 275 1289 932
Fragments arranged by size:
1289 932 275
StyI C'CwwG_G
Cuts at: 0 446 2496
Size: 446 2050
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 413 1300 2496
Size: 413 887 1196
Fragments arranged by size:
1196 887 413
TatI w'GTAC_w
Cuts at: 0 2009 2303 2496
Size: 2009 294 193
Fragments arranged by size:
2009 294 193
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 223 255 2496
Size: 223 32 2241
Fragments arranged by size:
2241 223 32
XhoI C'TCGA_G
Cuts at: 0 267 2496
Size: 267 2229
Enzymes that do cut:
AarI AcuI AflII AflIII AlfI AlwNI ApoI AvaI
BamHI BanI BanII BbeI BbsI BbvCI BfrBI BglI
BglII BlpI Bme1580I BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrDI BsrFI
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I
FalI HaeII Hin4I HincII HindIII HpaI KasI MmeI
MslI MspA1I NaeI NarI NcoI NgoMIV NsiI NspI
PasI PflMI PfoI PmlI PpuMI PstI PvuII SacI
SfcI SfoI SgrAI SmlI SphI StuI StyI TaqII
TatI TstI XhoI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AgeI AhdI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BcgI BciVI BclI BmgBI BmrI BmtI BplI BsiEI
BsiWI BsrBI BsrGI BssHII BstBI BstZ17I ClaI DraI
DraIII DrdI EagI EciI EcoRI EcoRV FseI FspI
FspAI KpnI MfeI MluI MscI NdeI NheI NotI
NruI PacI PciI PmeI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SmaI SnaBI SpeI
SrfI SspI SwaI Tth111I XbaI XcmI XmaI XmnI
ZraI