(Linear) (Six-base) MAPSORT of: KIAA0448.seq  Check: 1323  from: 1  to: 1068



KIAA0448.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     689    1068 
   Size:        689     379
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     782    1068 
   Size:        782     286
 
AflIII A'CryG_T

Cuts at:      0     423    1068 
   Size:        423     645
 
AhdI GACnn_n'nnGTC

Cuts at:      0     145    1068 
   Size:        145     923
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     928     960    1068 
   Size:        928      32     108

  Fragments arranged by size: 

                 928     108      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      42      99    1068 
   Size:         42      57     969

  Fragments arranged by size: 

                 969      57      42
 
ApoI r'AATT_y

Cuts at:      0     448     952    1068 
   Size:        448     504     116

  Fragments arranged by size: 

                 504     448     116
 
AseI AT'TA_AT

Cuts at:      0     479     734    1068 
   Size:        479     255     334

  Fragments arranged by size: 

                 479     334     255
 
BamHI G'GATC_C

Cuts at:      0     492    1068 
   Size:        492     576
 
BanII G_rGCy'C

Cuts at:      0       8     987    1068 
   Size:          8     979      81

  Fragments arranged by size: 

                 979      81       8
 
BclI T'GATC_A

Cuts at:      0     224    1068 
   Size:        224     844
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     129    1068 
   Size:        129     939
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     614    1068 
   Size:        121     493     454

  Fragments arranged by size: 

                 493     454     121
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1004    1068 
   Size:       1004      64
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     928     958    1068 
   Size:        928      30     110

  Fragments arranged by size: 

                 928     110      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     118    1068 
   Size:        118     950
 
BsiHKAI G_wGCw'C

Cuts at:      0     630    1068 
   Size:        630     438
 
BsmI GAATG_Cn'

Cuts at:      0     685    1068 
   Size:        685     383
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     558    1068 
   Size:        558     510
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     630     987    1068 
   Size:          8     622     357      81

  Fragments arranged by size: 

                 622     357      81       8
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     689    1068 
   Size:        689     379
 
BsrBI CCG'CTC

Cuts at:      0     113    1068 
   Size:        113     955
 
BsrDI GCAATG_nn'

Cuts at:      0     349     799     899    1068 
   Size:        349     450     100     169

  Fragments arranged by size: 

                 450     349     169     100
 
BstYI r'GATC_y

Cuts at:      0      89     492    1068 
   Size:         89     403     576

  Fragments arranged by size: 

                 576     403      89
 
Bsu36I CC'TnA_GG

Cuts at:      0      10    1068 
   Size:         10    1058
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0      82    1068 
   Size:         82     986
 
EaeI y'GGCC_r

Cuts at:      0     666    1068 
   Size:        666     402
 
EarI CTCTTCn'nnn_

Cuts at:      0      79     206    1068 
   Size:         79     127     862

  Fragments arranged by size: 

                 862     127      79
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     121     614     782    1068 
   Size:        121     493     168     286

  Fragments arranged by size: 

                 493     286     168     121
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     131     163    1068 
   Size:        131      32     905

  Fragments arranged by size: 

                 905     131      32
 
MscI TGG'CCA

Cuts at:      0     668    1068 
   Size:        668     400
 
MspA1I CmG'CkG

Cuts at:      0      39    1068 
   Size:         39    1029
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      99    1068 
   Size:         99     969
 
PfoI T'CCnGG_A

Cuts at:      0     388    1068 
   Size:        388     680
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     928     960    1068 
   Size:        928      32     108

  Fragments arranged by size: 

                 928     108      32
 
PsiI TTA'TAA

Cuts at:      0     233    1068 
   Size:        233     835
 
PvuII CAG'CTG

Cuts at:      0      39    1068 
   Size:         39    1029
 
SapI GCTCTTCn'nnn_

Cuts at:      0      79    1068 
   Size:         79     989
 
SfcI C'TryA_G

Cuts at:      0     910    1068 
   Size:        910     158
 
SspI AAT'ATT

Cuts at:      0     742    1068 
   Size:        742     326
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     638    1068 
   Size:        638     430
 
XmnI GAAnn'nnTTC

Cuts at:      0     743     943    1068 
   Size:        743     200     125

  Fragments arranged by size: 

                 743     200     125
 

 Enzymes that do cut:

     AarI     AcuI   AflIII     AhdI     AloI    AlwNI     ApoI     AseI
    BamHI    BanII     BclI     BplI     BpmI     BsaI    BsaXI    BseRI
  BsiHKAI     BsmI    BsmBI Bsp1286I    BspMI    BsrBI    BsrDI    BstYI
   Bsu36I    BtgZI     EaeI     EarI  Eco57MI    Hin4I     MscI   MspA1I
    PflMI     PfoI     PpiI     PsiI    PvuII     SapI     SfcI     SspI
     XcmI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AlfI     ApaI    ApaLI     AscI    AsiSI     AvaI    AvrII     BaeI
     BanI     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI   Bpu10I    BpuEI
    BsaAI    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI
    BspEI    BspHI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI  BstZ17I     BtgI     BtsI     ClaI    CspCI     DraI
   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII
     HpaI     KasI     KpnI     MfeI     MluI     MmeI     MslI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI    PpuMI    PshAI
   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII     SacI    SacII
     SalI    SanDI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     StuI
     StyI     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XhoI
     XmaI     ZraI