Restriction map for
pF1KA0386
(Linear) (Six-base) MAPSORT of: KIAA0386.seq Check: 2485 from: 1 to: 3204
KIAA0386.orf
With 182 enzymes: SgfI *
February 28, 2007 11:20 ..
AccI GT'mk_AC
Cuts at: 0 230 896 2477 3204
Size: 230 666 1581 727
Fragments arranged by size:
1581 727 666 230
Acc65I G'GTAC_C
Cuts at: 0 913 1036 3204
Size: 913 123 2168
Fragments arranged by size:
2168 913 123
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 175 991 1158 1281 1519 1799 2174 3083
Size: 175 816 167 123 238 280 375 909
Cuts at: 3083 3204
Size: 121
Fragments arranged by size:
909 816 375 280 238 175 167 123
121
AfeI AGC'GCT
Cuts at: 0 2147 3204
Size: 2147 1057
AhdI GACnn_n'nnGTC
Cuts at: 0 3047 3204
Size: 3047 157
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3049 3204
Size: 3049 155
AlwNI CAG_nnn'CTG
Cuts at: 0 985 1576 1881 2083 2745 3204
Size: 985 591 305 202 662 459
Fragments arranged by size:
985 662 591 459 305 202
ApaI G_GGCC'C
Cuts at: 0 38 3204
Size: 38 3166
ApaLI G'TGCA_C
Cuts at: 0 570 3204
Size: 570 2634
ApoI r'AATT_y
Cuts at: 0 120 607 671 1899 2416 2591 3142 3204
Size: 120 487 64 1228 517 175 551 62
Fragments arranged by size:
1228 551 517 487 175 120 64 62
AvaI C'yCGr_G
Cuts at: 0 515 3149 3204
Size: 515 2634 55
Fragments arranged by size:
2634 515 55
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 897 930 3204
Size: 897 33 2274
Fragments arranged by size:
2274 897 33
BamHI G'GATC_C
Cuts at: 0 10 3204
Size: 10 3194
BanI G'GyrC_C
Cuts at: 0 463 913 1036 1725 2240 3204
Size: 463 450 123 689 515 964
Fragments arranged by size:
964 689 515 463 450 123
BanII G_rGCy'C
Cuts at: 0 38 809 1223 1523 1652 3204
Size: 38 771 414 300 129 1552
Fragments arranged by size:
1552 771 414 300 129 38
BbsI GAAGACnn'nnnn_
Cuts at: 0 1514 1940 2162 3204
Size: 1514 426 222 1042
Fragments arranged by size:
1514 1042 426 222
BbvCI CC'TCA_GC
Cuts at: 0 206 1402 3204
Size: 206 1196 1802
Fragments arranged by size:
1802 1196 206
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2244 2278 3204
Size: 2244 34 926
Fragments arranged by size:
2244 926 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 451 956 3204
Size: 451 505 2248
Fragments arranged by size:
2248 505 451
BglI GCCn_nnn'nGGC
Cuts at: 0 33 1110 3204
Size: 33 1077 2094
Fragments arranged by size:
2094 1077 33
BlpI GC'TnA_GC
Cuts at: 0 1291 1446 1583 2606 3204
Size: 1291 155 137 1023 598
Fragments arranged by size:
1291 1023 598 155 137
Bme1580I G_kGCm'C
Cuts at: 0 38 574 3204
Size: 38 536 2630
Fragments arranged by size:
2630 536 38
BmrI ACTGGGnnnn_n'
Cuts at: 0 9 2560 3204
Size: 9 2551 644
Fragments arranged by size:
2551 644 9
BmtI G_CTAG'C
Cuts at: 0 820 3204
Size: 820 2384
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1659 3204
Size: 1659 1545
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 67 286 415 439 677 2230 2533
Size: 50 17 219 129 24 238 1553 303
Cuts at: 2533 2607 2716 2956 2962 3204
Size: 74 109 240 6 242
Fragments arranged by size:
1553 303 242 240 238 219 129 109
74 50 24 17 6
Bpu10I CC'TnA_GC
Cuts at: 0 206 1402 2707 3204
Size: 206 1196 1305 497
Fragments arranged by size:
1305 1196 497 206
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 1143 1618 1636 2268 3031 3204
Size: 118 1025 475 18 632 763 173
Fragments arranged by size:
1025 763 632 475 173 118 18
BsaI GGTCTCn'nnnn_
Cuts at: 0 847 3204
Size: 847 2357
BsaBI GATnn'nnATC
Cuts at: 0 260 3204
Size: 260 2944
BsaHI Gr'CG_yC
Cuts at: 0 390 450 2684 3204
Size: 390 60 2234 520
Fragments arranged by size:
2234 520 390 60
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1171 1201 3204
Size: 1171 30 2003
Fragments arranged by size:
2003 1171 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1026 1285 1531 1651 2035 2038 3204
Size: 1026 259 246 120 384 3 1166
Fragments arranged by size:
1166 1026 384 259 246 120 3
BseYI C'CCAG_C
Cuts at: 0 600 740 985 1576 2487 2797 2813 3204
Size: 600 140 245 591 911 310 16 391
Fragments arranged by size:
911 600 591 391 310 245 140 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1141 2238 2287 3204
Size: 1141 1097 49 917
Fragments arranged by size:
1141 1097 917 49
BsiHKAI G_wGCw'C
Cuts at: 0 574 809 1223 1589 1652 3204
Size: 574 235 414 366 63 1552
Fragments arranged by size:
1552 574 414 366 235 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 381 3204
Size: 381 2823
Bsp1286I G_dGCh'C
Cuts at: 0 38 574 809 1223 1523 1589 1652 3204
Size: 38 536 235 414 300 66 63 1552
Fragments arranged by size:
1552 536 414 300 235 66 63 38
BspHI T'CATG_A
Cuts at: 0 677 1895 3204
Size: 677 1218 1309
Fragments arranged by size:
1309 1218 677
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1190 1313 1539 1934 3204
Size: 1190 123 226 395 1270
Fragments arranged by size:
1270 1190 395 226 123
BsrBI CCG'CTC
Cuts at: 0 130 1169 3204
Size: 130 1039 2035
Fragments arranged by size:
2035 1039 130
BsrFI r'CCGG_y
Cuts at: 0 689 3204
Size: 689 2515
BsrGI T'GTAC_A
Cuts at: 0 1025 3204
Size: 1025 2179
BssSI C'ACGA_G
Cuts at: 0 2263 3204
Size: 2263 941
BstBI TT'CG_AA
Cuts at: 0 2574 3204
Size: 2574 630
BstXI CCAn_nnnn'nTGG
Cuts at: 0 582 3204
Size: 582 2622
BstYI r'GATC_y
Cuts at: 0 10 1789 1878 2336 3204
Size: 10 1779 89 458 868
Fragments arranged by size:
1779 868 458 89 10
Bsu36I CC'TnA_GG
Cuts at: 0 143 881 978 1192 1569 3204
Size: 143 738 97 214 377 1635
Fragments arranged by size:
1635 738 377 214 143 97
BtgI C'CryG_G
Cuts at: 0 2679 2732 3204
Size: 2679 53 472
Fragments arranged by size:
2679 472 53
EaeI y'GGCC_r
Cuts at: 0 687 1733 2717 3204
Size: 687 1046 984 487
Fragments arranged by size:
1046 984 687 487
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 2069 3204
Size: 2069 1135
EcoICRI GAG'CTC
Cuts at: 0 807 1221 1650 3204
Size: 807 414 429 1554
Fragments arranged by size:
1554 807 429 414
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 50 67 175 286 415 439 677 991
Size: 50 17 108 111 129 24 238 314
Cuts at: 991 1158 1281 1519 1799 2174 2230 2533 2607
Size: 167 123 238 280 375 56 303 74
Cuts at: 2607 2716 2956 2962 3083 3204
Size: 109 240 6 121 121
Fragments arranged by size:
375 314 303 280 240 238 238 167
129 123 121 121 111 109 108 74
56 50 24 17 6
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1074 1191 1501 3204
Size: 1074 117 310 1703
Fragments arranged by size:
1703 1074 310 117
EcoO109I rG'GnC_Cy
Cuts at: 0 34 236 3204
Size: 34 202 2968
Fragments arranged by size:
2968 202 34
EcoRI G'AATT_C
Cuts at: 0 607 3204
Size: 607 2597
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 501 3204
Size: 501 2703
HaeII r_GCGC'y
Cuts at: 0 986 2149 3204
Size: 986 1163 1055
Fragments arranged by size:
1163 1055 986
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 612 644 1171 1203 1429 1461 1474 1506
Size: 612 32 527 32 226 32 13 32
Cuts at: 1506 2823 2855 3033 3065 3204
Size: 1317 32 178 32 139
Fragments arranged by size:
1317 612 527 226 178 139 32 32
32 32 32 32 13
HincII GTy'rAC
Cuts at: 0 296 897 2538 3204
Size: 296 601 1641 666
Fragments arranged by size:
1641 666 601 296
HindIII A'AGCT_T
Cuts at: 0 431 1806 1986 2323 3204
Size: 431 1375 180 337 881
Fragments arranged by size:
1375 881 431 337 180
KpnI G_GTAC'C
Cuts at: 0 917 1040 3204
Size: 917 123 2164
Fragments arranged by size:
2164 917 123
MfeI C'AATT_G
Cuts at: 0 371 3133 3204
Size: 371 2762 71
Fragments arranged by size:
2762 371 71
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1379 3147 3204
Size: 1379 1768 57
Fragments arranged by size:
1768 1379 57
MscI TGG'CCA
Cuts at: 0 1735 2719 3204
Size: 1735 984 485
Fragments arranged by size:
1735 984 485
MslI CAynn'nnrTG
Cuts at: 0 580 619 2138 3031 3204
Size: 580 39 1519 893 173
Fragments arranged by size:
1519 893 580 173 39
MspA1I CmG'CkG
Cuts at: 0 77 696 1698 2362 2491 2511 2715 3204
Size: 77 619 1002 664 129 20 204 489
Fragments arranged by size:
1002 664 619 489 204 129 77 20
NaeI GCC'GGC
Cuts at: 0 691 3204
Size: 691 2513
NcoI C'CATG_G
Cuts at: 0 2732 3204
Size: 2732 472
NdeI CA'TA_TG
Cuts at: 0 3126 3204
Size: 3126 78
NgoMIV G'CCGG_C
Cuts at: 0 689 3204
Size: 689 2515
NheI G'CTAG_C
Cuts at: 0 816 3204
Size: 816 2388
PasI CC'CwG_GG
Cuts at: 0 1467 3204
Size: 1467 1737
PflMI CCAn_nnn'nTGG
Cuts at: 0 2346 2595 3204
Size: 2346 249 609
Fragments arranged by size:
2346 609 249
PfoI T'CCnGG_A
Cuts at: 0 83 455 654 1456 2550 3204
Size: 83 372 199 802 1094 654
Fragments arranged by size:
1094 802 654 372 199 83
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1576 1608 3204
Size: 1576 32 1596
Fragments arranged by size:
1596 1576 32
PspOMI G'GGCC_C
Cuts at: 0 34 3204
Size: 34 3170
PstI C_TGCA'G
Cuts at: 0 1722 1789 2464 3204
Size: 1722 67 675 740
Fragments arranged by size:
1722 740 675 67
PvuII CAG'CTG
Cuts at: 0 1698 2362 2511 2715 3204
Size: 1698 664 149 204 489
Fragments arranged by size:
1698 664 489 204 149
SacI G_AGCT'C
Cuts at: 0 809 1223 1652 3204
Size: 809 414 429 1552
Fragments arranged by size:
1552 809 429 414
SalI G'TCGA_C
Cuts at: 0 895 3204
Size: 895 2309
ScaI AGT'ACT
Cuts at: 0 2251 2825 3204
Size: 2251 574 379
Fragments arranged by size:
2251 574 379
SexAI A'CCwGG_T
Cuts at: 0 940 3204
Size: 940 2264
SfcI C'TryA_G
Cuts at: 0 231 1718 1785 2460 3204
Size: 231 1487 67 675 744
Fragments arranged by size:
1487 744 675 231 67
SmaI CCC'GGG
Cuts at: 0 517 3204
Size: 517 2687
SmlI C'TyrA_G
Cuts at: 0 133 1122 1597 1651 2283 3010 3149 3204
Size: 133 989 475 54 632 727 139 55
Fragments arranged by size:
989 727 632 475 139 133 55 54
StuI AGG'CCT
Cuts at: 0 1642 2932 3204
Size: 1642 1290 272
Fragments arranged by size:
1642 1290 272
StyI C'CwwG_G
Cuts at: 0 909 1205 2732 3204
Size: 909 296 1527 472
Fragments arranged by size:
1527 909 472 296
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 325 2132 2272 3204
Size: 325 1807 140 932
Fragments arranged by size:
1807 932 325 140
TatI w'GTAC_w
Cuts at: 0 339 554 1025 2249 2823 3204
Size: 339 215 471 1224 574 381
Fragments arranged by size:
1224 574 471 381 339 215
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 930 962 3204
Size: 930 32 2242
Fragments arranged by size:
2242 930 32
XbaI T'CTAG_A
Cuts at: 0 1797 3204
Size: 1797 1407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1464 2805 3204
Size: 1464 1341 399
Fragments arranged by size:
1464 1341 399
XhoI C'TCGA_G
Cuts at: 0 3149 3204
Size: 3149 55
XmaI C'CCGG_G
Cuts at: 0 515 3204
Size: 515 2689
XmnI GAAnn'nnTTC
Cuts at: 0 1619 3204
Size: 1619 1585
Enzymes that do cut:
AccI Acc65I AcuI AfeI AhdI AlfI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BcgI BciVI BglI BlpI Bme1580I BmrI BmtI
BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspHI BspMI
BsrBI BsrFI BsrGI BssSI BstBI BstXI BstYI Bsu36I
BtgI EaeI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
FalI HaeII Hin4I HincII HindIII KpnI MfeI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
PasI PflMI PfoI PpiI PspOMI PstI PvuII SacI
SalI ScaI SexAI SfcI SmaI SmlI StuI StyI
TaqII TatI TstI XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AarI AatII AclI AflII AflIII AgeI AleI AloI
AscI AseI AsiSI AvrII BbeI BclI BfrBI BglII
BmgBI BsaAI BsaWI BsiEI BsiWI BsmI BspEI BsrDI
BssHII BstAPI BstEII BstZ17I BtgZI BtsI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoRV FseI FspI
FspAI HpaI KasI MluI NarI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpuMI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SanDI SapI SbfI
SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI
SspI SwaI Tth111I ZraI