Restriction map for
pF1KSDA0364
(Linear) (Six-base) MAPSORT of: KIAA0364.seq Check: 2595 from: 1 to: 3981
KIAA0364.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 205 643 1273 1498 1867 3211 3499
Size: 58 147 438 630 225 369 1344 288
Cuts at: 3499 3981
Size: 482
Fragments arranged by size:
1344 630 482 438 369 288 225 147
58
AgeI A'CCGG_T
Cuts at: 0 284 3981
Size: 284 3697
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 193 1577 2168 3981
Size: 193 1384 591 1813
Fragments arranged by size:
1813 1384 591 193
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2057 2089 2693 2725 3981
Size: 2057 32 604 32 1256
Fragments arranged by size:
2057 1256 604 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 484 2441 2620 3086 3727 3981
Size: 484 1957 179 466 641 254
Fragments arranged by size:
1957 641 484 466 254 179
ApaI G_GGCC'C
Cuts at: 0 680 3981
Size: 680 3301
ApaLI G'TGCA_C
Cuts at: 0 3741 3981
Size: 3741 240
ApoI r'AATT_y
Cuts at: 0 810 1357 2581 3544 3981
Size: 810 547 1224 963 437
Fragments arranged by size:
1224 963 810 547 437
AvaI C'yCGr_G
Cuts at: 0 2351 3981
Size: 2351 1630
BamHI G'GATC_C
Cuts at: 0 209 3016 3981
Size: 209 2807 965
Fragments arranged by size:
2807 965 209
BanI G'GyrC_C
Cuts at: 0 143 253 345 687 713 1915 2347 2942
Size: 143 110 92 342 26 1202 432 595
Cuts at: 2942 3916 3981
Size: 974 65
Fragments arranged by size:
1202 974 595 432 342 143 110 92
65 26
BanII G_rGCy'C
Cuts at: 0 611 680 1475 1985 2264 2552 2570 2706
Size: 611 69 795 510 279 288 18 136
Cuts at: 2706 2903 3282 3981
Size: 197 379 699
Fragments arranged by size:
795 699 611 510 379 288 279 197
136 69 18
BbeI G_GCGC'C
Cuts at: 0 1919 3981
Size: 1919 2062
BbsI GAAGACnn'nnnn_
Cuts at: 0 46 767 796 1055 1174 3359 3981
Size: 46 721 29 259 119 2185 622
Fragments arranged by size:
2185 721 622 259 119 46 29
BbvCI CC'TCA_GC
Cuts at: 0 3578 3981
Size: 3578 403
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2664 2698 3981
Size: 2664 34 1283
Fragments arranged by size:
2664 1283 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2962 3981
Size: 2962 1019
BglI GCCn_nnn'nGGC
Cuts at: 0 1820 3981
Size: 1820 2161
BglII A'GATC_T
Cuts at: 0 3431 3981
Size: 3431 550
BlpI GC'TnA_GC
Cuts at: 0 2897 3981
Size: 2897 1084
Bme1580I G_kGCm'C
Cuts at: 0 258 680 2352 3745 3981
Size: 258 422 1672 1393 236
Fragments arranged by size:
1672 1393 422 258 236
BmrI ACTGGGnnnn_n'
Cuts at: 0 317 515 953 1979 2258 2897 3981
Size: 317 198 438 1026 279 639 1084
Fragments arranged by size:
1084 1026 639 438 317 279 198
BmtI G_CTAG'C
Cuts at: 0 3811 3981
Size: 3811 170
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 907 3981
Size: 907 3074
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 713 1336 2296 3448 3646 3724 3981
Size: 713 623 960 1152 198 78 257
Fragments arranged by size:
1152 960 713 623 257 198 78
Bpu10I CC'TnA_GC
Cuts at: 0 1742 1748 2303 2879 3281 3578 3734 3981
Size: 1742 6 555 576 402 297 156 247
Fragments arranged by size:
1742 576 555 402 297 247 156 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1628 2017 3981
Size: 1628 389 1964
Fragments arranged by size:
1964 1628 389
BsaI GGTCTCn'nnnn_
Cuts at: 0 382 1756 3863 3981
Size: 382 1374 2107 118
Fragments arranged by size:
2107 1374 382 118
BsaHI Gr'CG_yC
Cuts at: 0 1916 3981
Size: 1916 2065
BsaWI w'CCGG_w
Cuts at: 0 84 284 2075 3981
Size: 84 200 1791 1906
Fragments arranged by size:
1906 1791 200 84
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1825 1855 2057 2087 2585 2615 2693 2723
Size: 1825 30 202 30 498 30 78 30
Cuts at: 2723 3406 3436 3590 3620 3981
Size: 683 30 154 30 361
Fragments arranged by size:
1825 683 498 361 202 154 78 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1978 3981
Size: 1978 2003
BseYI C'CCAG_C
Cuts at: 0 610 1632 2551 3172 3185 3478 3981
Size: 610 1022 919 621 13 293 503
Fragments arranged by size:
1022 919 621 610 503 293 13
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1827 2483 2962 3981
Size: 1827 656 479 1019
Fragments arranged by size:
1827 1019 656 479
BsiHKAI G_wGCw'C
Cuts at: 0 2570 3745 3981
Size: 2570 1175 236
Fragments arranged by size:
2570 1175 236
BsmI GAATG_Cn'
Cuts at: 0 65 3037 3981
Size: 65 2972 944
Fragments arranged by size:
2972 944 65
Bsp1286I G_dGCh'C
Cuts at: 0 258 611 680 1475 1985 2264 2352 2552
Size: 258 353 69 795 510 279 88 200
Cuts at: 2552 2570 2706 2903 3282 3745 3981
Size: 18 136 197 379 463 236
Fragments arranged by size:
795 510 463 379 353 279 258 236
200 197 136 88 69 18
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 265 1881 3981
Size: 265 1616 2100
Fragments arranged by size:
2100 1616 265
BsrBI CCG'CTC
Cuts at: 0 397 3981
Size: 397 3584
BsrDI GCAATG_nn'
Cuts at: 0 1704 3878 3981
Size: 1704 2174 103
Fragments arranged by size:
2174 1704 103
BsrFI r'CCGG_y
Cuts at: 0 284 2952 3981
Size: 284 2668 1029
Fragments arranged by size:
2668 1029 284
BssSI C'ACGA_G
Cuts at: 0 2569 3981
Size: 2569 1412
BstEII G'GTnAC_C
Cuts at: 0 1650 2337 3981
Size: 1650 687 1644
Fragments arranged by size:
1650 1644 687
BstXI CCAn_nnnn'nTGG
Cuts at: 0 844 966 2149 2334 2604 2767 2910 3981
Size: 844 122 1183 185 270 163 143 1071
Fragments arranged by size:
1183 1071 844 270 185 163 143 122
BstYI r'GATC_y
Cuts at: 0 209 833 3016 3362 3431 3981
Size: 209 624 2183 346 69 550
Fragments arranged by size:
2183 624 550 346 209 69
Bsu36I CC'TnA_GG
Cuts at: 0 545 3981
Size: 545 3436
BtgI C'CryG_G
Cuts at: 0 429 1783 2884 2918 2958 3197 3981
Size: 429 1354 1101 34 40 239 784
Fragments arranged by size:
1354 1101 784 429 239 40 34
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1447 3981
Size: 1447 2534
BtsI GCAGTG_nn'
Cuts at: 0 1473 1947 3347 3981
Size: 1473 474 1400 634
Fragments arranged by size:
1473 1400 634 474
ClaI AT'CG_AT
Cuts at: 0 1085 3981
Size: 1085 2896
DraI TTT'AAA
Cuts at: 0 465 3981
Size: 465 3516
DraIII CAC_nnn'GTG
Cuts at: 0 599 3981
Size: 599 3382
DrdI GACnn_nn'nnGTC
Cuts at: 0 312 2817 3981
Size: 312 2505 1164
Fragments arranged by size:
2505 1164 312
EaeI y'GGCC_r
Cuts at: 0 515 1249 3981
Size: 515 734 2732
Fragments arranged by size:
2732 734 515
EarI CTCTTCn'nnn_
Cuts at: 0 404 1666 1709 2157 2632 3230 3861 3884
Size: 404 1262 43 448 475 598 631 23
Cuts at: 3884 3981
Size: 97
Fragments arranged by size:
1262 631 598 475 448 404 97 43
23
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 202 3981
Size: 202 3779
EcoICRI GAG'CTC
Cuts at: 0 2568 3981
Size: 2568 1413
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 58 205 643 713 1273 1336 1498 1867
Size: 58 147 438 70 560 63 162 369
Cuts at: 1867 2296 3211 3448 3499 3646 3724 3981
Size: 429 915 237 51 147 78 257
Fragments arranged by size:
915 560 438 429 369 257 237 162
147 147 78 70 63 58 51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1176 2692 3268 3981
Size: 1176 1516 576 713
Fragments arranged by size:
1516 1176 713 576
EcoO109I rG'GnC_Cy
Cuts at: 0 117 1412 2438 2617 3526 3724 3981
Size: 117 1295 1026 179 909 198 257
Fragments arranged by size:
1295 1026 909 257 198 179 117
EcoRI G'AATT_C
Cuts at: 0 2581 3981
Size: 2581 1400
EcoRV GAT'ATC
Cuts at: 0 164 3678 3981
Size: 164 3514 303
Fragments arranged by size:
3514 303 164
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 387 2328 3981
Size: 387 1941 1653
Fragments arranged by size:
1941 1653 387
HaeII r_GCGC'y
Cuts at: 0 1919 3981
Size: 1919 2062
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 63 95 381 413 654 686 729 761
Size: 63 32 286 32 241 32 43 32
Cuts at: 761 839 871 1081 1113 1364 1396 1598 1630
Size: 78 32 210 32 251 32 202 32
Cuts at: 1630 1857 1889 2939 2971 3590 3622 3667 3699
Size: 227 32 1050 32 619 32 45 32
Cuts at: 3699 3981
Size: 282
Fragments arranged by size:
1050 619 286 282 251 241 227 210
202 78 63 45 43 32 32 32
32 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 417 1395 3981
Size: 417 978 2586
Fragments arranged by size:
2586 978 417
HpaI GTT'AAC
Cuts at: 0 417 3981
Size: 417 3564
KasI G'GCGC_C
Cuts at: 0 1915 3981
Size: 1915 2066
MfeI C'AATT_G
Cuts at: 0 2364 3981
Size: 2364 1617
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 128 320 1582 2139 2748 3981
Size: 128 192 1262 557 609 1233
Fragments arranged by size:
1262 1233 609 557 192 128
MscI TGG'CCA
Cuts at: 0 517 1251 3981
Size: 517 734 2730
Fragments arranged by size:
2730 734 517
MslI CAynn'nnrTG
Cuts at: 0 70 1180 1466 2485 3001 3981
Size: 70 1110 286 1019 516 980
Fragments arranged by size:
1110 1019 980 516 286 70
MspA1I CmG'CkG
Cuts at: 0 2219 2274 2516 2746 3083 3647 3981
Size: 2219 55 242 230 337 564 334
Fragments arranged by size:
2219 564 337 334 242 230 55
NarI GG'CG_CC
Cuts at: 0 1916 3981
Size: 1916 2065
NcoI C'CATG_G
Cuts at: 0 429 2884 2918 2958 3197 3981
Size: 429 2455 34 40 239 784
Fragments arranged by size:
2455 784 429 239 40 34
NdeI CA'TA_TG
Cuts at: 0 2387 3981
Size: 2387 1594
NheI G'CTAG_C
Cuts at: 0 3807 3981
Size: 3807 174
PflMI CCAn_nnn'nTGG
Cuts at: 0 374 599 730 941 1015 2190 3818 3981
Size: 374 225 131 211 74 1175 1628 163
Fragments arranged by size:
1628 1175 374 225 211 163 131 74
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2057 2089 2693 2725 3981
Size: 2057 32 604 32 1256
Fragments arranged by size:
2057 1256 604 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1412 2438 2617 3526 3981
Size: 1412 1026 179 909 455
Fragments arranged by size:
1412 1026 909 455 179
PspOMI G'GGCC_C
Cuts at: 0 676 3981
Size: 676 3305
PstI C_TGCA'G
Cuts at: 0 443 3652 3981
Size: 443 3209 329
Fragments arranged by size:
3209 443 329
PvuII CAG'CTG
Cuts at: 0 2219 2516 2746 3083 3647 3981
Size: 2219 297 230 337 564 334
Fragments arranged by size:
2219 564 337 334 297 230
SacI G_AGCT'C
Cuts at: 0 2570 3981
Size: 2570 1411
SapI GCTCTTCn'nnn_
Cuts at: 0 404 1666 3981
Size: 404 1262 2315
Fragments arranged by size:
2315 1262 404
ScaI AGT'ACT
Cuts at: 0 1313 3981
Size: 1313 2668
SfcI C'TryA_G
Cuts at: 0 439 1122 1140 1416 2790 3366 3642 3648
Size: 439 683 18 276 1374 576 276 6
Cuts at: 3648 3746 3981
Size: 98 235
Fragments arranged by size:
1374 683 576 439 276 276 235 98
18 6
SfoI GGC'GCC
Cuts at: 0 1917 3981
Size: 1917 2064
SmlI C'TyrA_G
Cuts at: 0 1643 1996 3981
Size: 1643 353 1985
Fragments arranged by size:
1985 1643 353
StuI AGG'CCT
Cuts at: 0 3667 3981
Size: 3667 314
StyI C'CwwG_G
Cuts at: 0 121 429 717 1911 2658 2884 2918 2958
Size: 121 308 288 1194 747 226 34 40
Cuts at: 2958 3197 3794 3981
Size: 239 597 187
Fragments arranged by size:
1194 747 597 308 288 239 226 187
121 40 34
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 395 2850 3426 3981
Size: 395 2455 576 555
Fragments arranged by size:
2455 576 555 395
TatI w'GTAC_w
Cuts at: 0 1311 3981
Size: 1311 2670
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3406 3438 3981
Size: 3406 32 543
Fragments arranged by size:
3406 543 32
Tth111I GACn'n_nGTC
Cuts at: 0 3604 3981
Size: 3604 377
XbaI T'CTAG_A
Cuts at: 0 333 3552 3981
Size: 333 3219 429
Fragments arranged by size:
3219 429 333
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 502 1773 2271 2559 3981
Size: 502 1271 498 288 1422
Fragments arranged by size:
1422 1271 502 498 288
XmnI GAAnn'nnTTC
Cuts at: 0 43 1815 3548 3981
Size: 43 1772 1733 433
Fragments arranged by size:
1772 1733 433 43
Enzymes that do cut:
AcuI AgeI AlfI AloI AlwNI ApaI ApaLI ApoI
AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI
BciVI BglI BglII BlpI Bme1580I BmrI BmtI BplI
BpmI Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DraIII DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI HaeII
Hin4I HincII HpaI KasI MfeI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI PflMI PpiI PpuMI
PspOMI PstI PvuII SacI SapI ScaI SfcI SfoI
SmlI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AscI AseI AsiSI AvrII BaeI BclI
BfrBI BmgBI BsaAI BsaBI BsiEI BsiWI BsmBI BspEI
BspHI BsrGI BssHII BstAPI BstBI BstZ17I CspCI EagI
FseI FspI FspAI HindIII KpnI MluI NaeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SbfI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI XhoI
XmaI ZraI