Restriction map for
pF1KSDA0362
(Linear) (Six-base) MAPSORT of: KIAA0362.seq Check: 8300 from: 1 to: 3288
KIAA0362.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 1184 3288
Size: 166 1018 2104
Fragments arranged by size:
2104 1018 166
AatII G_ACGT'C
Cuts at: 0 800 1408 3288
Size: 800 608 1880
Fragments arranged by size:
1880 800 608
AccI GT'mk_AC
Cuts at: 0 805 1286 2774 3288
Size: 805 481 1488 514
Fragments arranged by size:
1488 805 514 481
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 1690 2323 2443 3288
Size: 300 1390 633 120 845
Fragments arranged by size:
1390 845 633 300 120
AfeI AGC'GCT
Cuts at: 0 244 3288
Size: 244 3044
AgeI A'CCGG_T
Cuts at: 0 1522 3288
Size: 1522 1766
AhdI GACnn_n'nnGTC
Cuts at: 0 801 1116 3288
Size: 801 315 2172
Fragments arranged by size:
2172 801 315
AleI CACnn'nnGTG
Cuts at: 0 1325 2551 3193 3288
Size: 1325 1226 642 95
Fragments arranged by size:
1325 1226 642 95
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 322 354 1630 1662 1771 1803 3288
Size: 322 32 1276 32 109 32 1485
Fragments arranged by size:
1485 1276 322 109 32 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 750 1047 1059 2337 2369 2522 2904 3288
Size: 750 297 12 1278 32 153 382 384
Fragments arranged by size:
1278 750 384 382 297 153 32 12
ApaI G_GGCC'C
Cuts at: 0 1233 1239 1817 3163 3288
Size: 1233 6 578 1346 125
Fragments arranged by size:
1346 1233 578 125 6
ApoI r'AATT_y
Cuts at: 0 553 2825 3288
Size: 553 2272 463
Fragments arranged by size:
2272 553 463
AvaI C'yCGr_G
Cuts at: 0 1232 1723 3288
Size: 1232 491 1565
Fragments arranged by size:
1565 1232 491
BanI G'GyrC_C
Cuts at: 0 582 1319 2588 2787 3081 3288
Size: 582 737 1269 199 294 207
Fragments arranged by size:
1269 737 582 294 207 199
BanII G_rGCy'C
Cuts at: 0 604 928 935 1233 1239 1316 1550 1817
Size: 604 324 7 298 6 77 234 267
Cuts at: 1817 1925 2407 2512 3163 3288
Size: 108 482 105 651 125
Fragments arranged by size:
651 604 482 324 298 267 234 125
108 105 77 7 6
BbeI G_GCGC'C
Cuts at: 0 2791 3288
Size: 2791 497
BbsI GAAGACnn'nnnn_
Cuts at: 0 1608 3288
Size: 1608 1680
BbvCI CC'TCA_GC
Cuts at: 0 920 1180 2381 3288
Size: 920 260 1201 907
Fragments arranged by size:
1201 920 907 260
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 690 724 1780 1814 3288
Size: 690 34 1056 34 1474
Fragments arranged by size:
1474 1056 690 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2998 3288
Size: 2998 290
BglI GCCn_nnn'nGGC
Cuts at: 0 1856 3288
Size: 1856 1432
BglII A'GATC_T
Cuts at: 0 1590 2174 2747 3288
Size: 1590 584 573 541
Fragments arranged by size:
1590 584 573 541
BlpI GC'TnA_GC
Cuts at: 0 598 1369 1866 2281 2401 3015 3288
Size: 598 771 497 415 120 614 273
Fragments arranged by size:
771 614 598 497 415 273 120
Bme1580I G_kGCm'C
Cuts at: 0 887 1233 1239 1817 3163 3288
Size: 887 346 6 578 1346 125
Fragments arranged by size:
1346 887 578 346 125 6
BmgBI CAC'GTC
Cuts at: 0 3249 3288
Size: 3249 39
BmrI ACTGGGnnnn_n'
Cuts at: 0 929 3288
Size: 929 2359
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 916 1069 1402 1420 1480 1767 1990 2173
Size: 916 153 333 18 60 287 223 183
Cuts at: 2173 2908 3019 3097 3288
Size: 735 111 78 191
Fragments arranged by size:
916 735 333 287 223 191 183 153
111 78 60 18
Bpu10I CC'TnA_GC
Cuts at: 0 920 1180 2381 2992 3288
Size: 920 260 1201 611 296
Fragments arranged by size:
1201 920 611 296 260
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2326 2922 2934 3288
Size: 2326 596 12 354
Fragments arranged by size:
2326 596 354 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 1513 3288
Size: 1513 1775
BsaAI yAC'GTr
Cuts at: 0 1605 1911 1947 3288
Size: 1605 306 36 1341
Fragments arranged by size:
1605 1341 306 36
BsaHI Gr'CG_yC
Cuts at: 0 448 797 1405 2788 3288
Size: 448 349 608 1383 500
Fragments arranged by size:
1383 608 500 448 349
BsaWI w'CCGG_w
Cuts at: 0 259 327 1522 1805 1820 3288
Size: 259 68 1195 283 15 1468
Fragments arranged by size:
1468 1195 283 259 68 15
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 664 694 1630 1660 1771 1801 1945 1975
Size: 664 30 936 30 111 30 144 30
Cuts at: 1975 3288
Size: 1313
Fragments arranged by size:
1313 936 664 144 111 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1216 1371 1654 1891 1894 1966 2650 2782
Size: 1216 155 283 237 3 72 684 132
Cuts at: 2782 3130 3157 3252 3288
Size: 348 27 95 36
Fragments arranged by size:
1216 684 348 283 237 155 132 95
72 36 27 3
BseYI C'CCAG_C
Cuts at: 0 58 126 373 989 3288
Size: 58 68 247 616 2299
Fragments arranged by size:
2299 616 247 68 58
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 994 1372 1723 2332 2584 3131 3283 3288
Size: 994 378 351 609 252 547 152 5
Fragments arranged by size:
994 609 547 378 351 252 152 5
BsiEI CG_ry'CG
Cuts at: 0 173 3194 3288
Size: 173 3021 94
Fragments arranged by size:
3021 173 94
BsiHKAI G_wGCw'C
Cuts at: 0 604 1175 1316 1550 1604 1925 2233 2407
Size: 604 571 141 234 54 321 308 174
Cuts at: 2407 2618 3288
Size: 211 670
Fragments arranged by size:
670 604 571 321 308 234 211 174
141 54
BsmI GAATG_Cn'
Cuts at: 0 1760 2260 3288
Size: 1760 500 1028
Fragments arranged by size:
1760 1028 500
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1396 3288
Size: 1396 1892
Bsp1286I G_dGCh'C
Cuts at: 0 604 887 928 935 1175 1233 1239 1316
Size: 604 283 41 7 240 58 6 77
Cuts at: 1316 1550 1604 1817 1925 2233 2407 2512 2618
Size: 234 54 213 108 308 174 105 106
Cuts at: 2618 3163 3288
Size: 545 125
Fragments arranged by size:
604 545 308 283 240 234 213 174
125 108 106 105 77 58 54 41
7 6
BspEI T'CCGG_A
Cuts at: 0 327 1805 3288
Size: 327 1478 1483
Fragments arranged by size:
1483 1478 327
BspHI T'CATG_A
Cuts at: 0 350 3288
Size: 350 2938
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 82 166 494 1184 1445 2306 2396 3288
Size: 82 84 328 690 261 861 90 892
Fragments arranged by size:
892 861 690 328 261 90 84 82
BsrBI CCG'CTC
Cuts at: 0 207 2207 3242 3288
Size: 207 2000 1035 46
Fragments arranged by size:
2000 1035 207 46
BsrFI r'CCGG_y
Cuts at: 0 416 585 1522 1707 1850 2461 2913 3288
Size: 416 169 937 185 143 611 452 375
Fragments arranged by size:
937 611 452 416 375 185 169 143
BssHII G'CGCG_C
Cuts at: 0 122 2761 3288
Size: 122 2639 527
Fragments arranged by size:
2639 527 122
BssSI C'ACGA_G
Cuts at: 0 499 2602 3205 3288
Size: 499 2103 603 83
Fragments arranged by size:
2103 603 499 83
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1059 2369 3288
Size: 1059 1310 919
Fragments arranged by size:
1310 1059 919
BstXI CCAn_nnnn'nTGG
Cuts at: 0 3171 3288
Size: 3171 117
BstYI r'GATC_y
Cuts at: 0 1538 1590 2174 2747 3288
Size: 1538 52 584 573 541
Fragments arranged by size:
1538 584 573 541 52
Bsu36I CC'TnA_GG
Cuts at: 0 904 2098 3086 3288
Size: 904 1194 988 202
Fragments arranged by size:
1194 988 904 202
BtgI C'CryG_G
Cuts at: 0 39 67 162 1220 1860 1982 3288
Size: 39 28 95 1058 640 122 1306
Fragments arranged by size:
1306 1058 640 122 95 39 28
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 447 528 2436 3288
Size: 447 81 1908 852
Fragments arranged by size:
1908 852 447 81
BtsI GCAGTG_nn'
Cuts at: 0 1060 2618 3288
Size: 1060 1558 670
Fragments arranged by size:
1558 1060 670
ClaI AT'CG_AT
Cuts at: 0 629 3288
Size: 629 2659
EaeI y'GGCC_r
Cuts at: 0 32 106 170 704 1190 1217 1682 1892
Size: 32 74 64 534 486 27 465 210
Cuts at: 1892 2564 3288
Size: 672 724
Fragments arranged by size:
724 672 534 486 465 210 74 64
32 27
EagI C'GGCC_G
Cuts at: 0 170 3288
Size: 170 3118
EarI CTCTTCn'nnn_
Cuts at: 0 2623 3288
Size: 2623 665
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1285 3214 3288
Size: 1285 1929 74
Fragments arranged by size:
1929 1285 74
EcoICRI GAG'CTC
Cuts at: 0 602 1314 1548 1923 2405 3288
Size: 602 712 234 375 482 883
Fragments arranged by size:
883 712 602 482 375 234
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 300 916 1069 1402 1420 1480 1690 1767
Size: 300 616 153 333 18 60 210 77
Cuts at: 1767 1990 2173 2323 2443 2908 3019 3097 3288
Size: 223 183 150 120 465 111 78 191
Fragments arranged by size:
616 465 333 300 223 210 191 183
153 150 120 111 78 77 60 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1821 2392 3288
Size: 1821 571 896
Fragments arranged by size:
1821 896 571
EcoO109I rG'GnC_Cy
Cuts at: 0 651 662 1185 1229 1236 1813 1814 2385
Size: 651 11 523 44 7 577 1 571
Cuts at: 2385 3160 3217 3279 3288
Size: 775 57 62 9
Fragments arranged by size:
775 651 577 571 523 62 57 44
11 9 7 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 261 2703 3288
Size: 261 2442 585
Fragments arranged by size:
2442 585 261
FspI TGC'GCA
Cuts at: 0 460 827 1903 3288
Size: 460 367 1076 1385
Fragments arranged by size:
1385 1076 460 367
FspAI rTGC'GCAy
Cuts at: 0 827 3288
Size: 827 2461
HaeII r_GCGC'y
Cuts at: 0 246 2401 2791 2887 3022 3288
Size: 246 2155 390 96 135 266
Fragments arranged by size:
2155 390 266 246 135 96
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 167 199 787 819 1102 1134 1192 1224
Size: 167 32 588 32 283 32 58 32
Cuts at: 1224 1321 1353 3288
Size: 97 32 1935
Fragments arranged by size:
1935 588 283 167 97 58 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 806 2021 3288
Size: 806 1215 1267
Fragments arranged by size:
1267 1215 806
KasI G'GCGC_C
Cuts at: 0 2787 3288
Size: 2787 501
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 563 2118 2258 3034 3158 3288
Size: 563 1555 140 776 124 130
Fragments arranged by size:
1555 776 563 140 130 124
MscI TGG'CCA
Cuts at: 0 1192 1894 2566 3288
Size: 1192 702 672 722
Fragments arranged by size:
1192 722 702 672
MslI CAynn'nnrTG
Cuts at: 0 406 1325 2551 3193 3288
Size: 406 919 1226 642 95
Fragments arranged by size:
1226 919 642 406 95
MspA1I CmG'CkG
Cuts at: 0 642 993 1862 1870 1984 2334 2586 2850
Size: 642 351 869 8 114 350 252 264
Cuts at: 2850 3288
Size: 438
Fragments arranged by size:
869 642 438 351 350 264 252 114
8
NaeI GCC'GGC
Cuts at: 0 1852 2915 3288
Size: 1852 1063 373
Fragments arranged by size:
1852 1063 373
NarI GG'CG_CC
Cuts at: 0 2788 3288
Size: 2788 500
NgoMIV G'CCGG_C
Cuts at: 0 1850 2913 3288
Size: 1850 1063 375
Fragments arranged by size:
1850 1063 375
NspI r_CATG'y
Cuts at: 0 105 1168 3288
Size: 105 1063 2120
Fragments arranged by size:
2120 1063 105
PflMI CCAn_nnn'nTGG
Cuts at: 0 1415 3075 3288
Size: 1415 1660 213
Fragments arranged by size:
1660 1415 213
PfoI T'CCnGG_A
Cuts at: 0 224 893 1088 1496 1541 1925 1967 2249
Size: 224 669 195 408 45 384 42 282
Cuts at: 2249 3288
Size: 1039
Fragments arranged by size:
1039 669 408 384 282 224 195 45
42
PmlI CAC'GTG
Cuts at: 0 1605 1911 3288
Size: 1605 306 1377
Fragments arranged by size:
1605 1377 306
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1630 1662 1771 1803 3288
Size: 1630 32 109 32 1485
Fragments arranged by size:
1630 1485 109 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 651 662 1185 2385 3288
Size: 651 11 523 1200 903
Fragments arranged by size:
1200 903 651 523 11
PspOMI G'GGCC_C
Cuts at: 0 1229 1235 1813 3159 3288
Size: 1229 6 578 1346 129
Fragments arranged by size:
1346 1229 578 129 6
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1261 1293 3288
Size: 1261 32 1995
Fragments arranged by size:
1995 1261 32
PstI C_TGCA'G
Cuts at: 0 148 383 491 755 917 1064 2393 2855
Size: 148 235 108 264 162 147 1329 462
Cuts at: 2855 3288
Size: 433
Fragments arranged by size:
1329 462 433 264 235 162 148 147
108
PvuII CAG'CTG
Cuts at: 0 642 993 2334 2850 3288
Size: 642 351 1341 516 438
Fragments arranged by size:
1341 642 516 438 351
SacI G_AGCT'C
Cuts at: 0 604 1316 1550 1925 2407 3288
Size: 604 712 234 375 482 881
Fragments arranged by size:
881 712 604 482 375 234
SacII CC_GC'GG
Cuts at: 0 1863 1985 3288
Size: 1863 122 1303
Fragments arranged by size:
1863 1303 122
SalI G'TCGA_C
Cuts at: 0 804 3288
Size: 804 2484
SanDI GG'GwC_CC
Cuts at: 0 662 3288
Size: 662 2626
SapI GCTCTTCn'nnn_
Cuts at: 0 2623 3288
Size: 2623 665
SbfI CC_TGCA'GG
Cuts at: 0 383 2393 3288
Size: 383 2010 895
Fragments arranged by size:
2010 895 383
ScaI AGT'ACT
Cuts at: 0 2188 3288
Size: 2188 1100
SfcI C'TryA_G
Cuts at: 0 144 379 487 751 913 1060 2389 2670
Size: 144 235 108 264 162 147 1329 281
Cuts at: 2670 2851 3288
Size: 181 437
Fragments arranged by size:
1329 437 281 264 235 181 162 147
144 108
SfoI GGC'GCC
Cuts at: 0 2789 3288
Size: 2789 499
SmaI CCC'GGG
Cuts at: 0 1234 3288
Size: 1234 2054
SmlI C'TyrA_G
Cuts at: 0 2341 2937 2949 3288
Size: 2341 596 12 339
Fragments arranged by size:
2341 596 339 12
SphI G_CATG'C
Cuts at: 0 105 3288
Size: 105 3183
SrfI GCCC'GGGC
Cuts at: 0 1234 3288
Size: 1234 2054
StuI AGG'CCT
Cuts at: 0 2027 3288
Size: 2027 1261
StyI C'CwwG_G
Cuts at: 0 2549 3288
Size: 2549 739
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 646 661 781 2542 2805 3288
Size: 646 15 120 1761 263 483
Fragments arranged by size:
1761 646 483 263 120 15
TatI w'GTAC_w
Cuts at: 0 2186 3288
Size: 2186 1102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1945 1977 3288
Size: 1945 32 1311
Fragments arranged by size:
1945 1311 32
Tth111I GACn'n_nGTC
Cuts at: 0 199 3288
Size: 199 3089
XmaI C'CCGG_G
Cuts at: 0 1232 3288
Size: 1232 2056
ZraI GAC'GTC
Cuts at: 0 798 1406 3288
Size: 798 608 1882
Fragments arranged by size:
1882 798 608
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AgeI AhdI AleI
AloI AlwNI ApaI ApoI AvaI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
ClaI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI
MmeI MscI MslI MspA1I NaeI NarI NgoMIV NspI
PflMI PfoI PmlI PpiI PpuMI PspOMI PsrI PstI
PvuII SacI SacII SalI SanDI SapI SbfI ScaI
SfcI SfoI SmaI SmlI SphI SrfI StuI StyI
TaqII TatI TstI Tth111I XmaI ZraI
Enzymes that do not cut:
Acc65I AclI AflII AflIII AlfI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BclI BfrBI BmtI BplI
BsaBI BsiWI BsrDI BsrGI BstBI BstEII BstZ17I CspCI
DraI DraIII DrdI EcoRI EcoRV FseI HindIII HpaI
KpnI MfeI MluI NcoI NdeI NheI NotI NruI
NsiI PacI PasI PciI PmeI PshAI PsiI PspXI
PvuI RsrII SexAI SfiI SgfI SgrAI SnaBI SpeI
SspI SwaI XbaI XcmI XhoI XmnI