Restriction map for
pF1KSDA0331
(Linear) (Six-base) MAPSORT of: KIAA0331.seq Check: 8315 from: 1 to: 2325
KIAA0331.orf
With 182 enzymes: SgfI *
October 24, 2006 15:12 ..
AatII G_ACGT'C
Cuts at: 0 1349 2325
Size: 1349 976
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1429 1775 2325
Size: 1429 346 550
Fragments arranged by size:
1429 550 346
AflIII A'CryG_T
Cuts at: 0 1034 1559 2325
Size: 1034 525 766
Fragments arranged by size:
1034 766 525
AhdI GACnn_n'nnGTC
Cuts at: 0 65 2325
Size: 65 2260
AlwNI CAG_nnn'CTG
Cuts at: 0 1945 2325
Size: 1945 380
ApaLI G'TGCA_C
Cuts at: 0 2199 2325
Size: 2199 126
ApoI r'AATT_y
Cuts at: 0 676 2134 2325
Size: 676 1458 191
Fragments arranged by size:
1458 676 191
AvaI C'yCGr_G
Cuts at: 0 1595 2325
Size: 1595 730
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1216 1249 2325
Size: 1216 33 1076
Fragments arranged by size:
1216 1076 33
BamHI G'GATC_C
Cuts at: 0 456 1458 2325
Size: 456 1002 867
Fragments arranged by size:
1002 867 456
BanII G_rGCy'C
Cuts at: 0 119 2325
Size: 119 2206
BbsI GAAGACnn'nnnn_
Cuts at: 0 716 1885 2325
Size: 716 1169 440
Fragments arranged by size:
1169 716 440
BbvCI CC'TCA_GC
Cuts at: 0 241 1328 2325
Size: 241 1087 997
Fragments arranged by size:
1087 997 241
BfrBI ATG'CAT
Cuts at: 0 314 2325
Size: 314 2011
BglII A'GATC_T
Cuts at: 0 484 2325
Size: 484 1841
BlpI GC'TnA_GC
Cuts at: 0 1706 2325
Size: 1706 619
Bme1580I G_kGCm'C
Cuts at: 0 17 2203 2325
Size: 17 2186 122
Fragments arranged by size:
2186 122 17
BmgBI CAC'GTC
Cuts at: 0 1855 2325
Size: 1855 470
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 311 2325
Size: 311 2014
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 425 606 778 2325
Size: 70 355 181 172 1547
Fragments arranged by size:
1547 355 181 172 70
Bpu10I CC'TnA_GC
Cuts at: 0 241 1328 2288 2325
Size: 241 1087 960 37
Fragments arranged by size:
1087 960 241 37
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1520 2325
Size: 1520 805
BsaI GGTCTCn'nnnn_
Cuts at: 0 1592 2325
Size: 1592 733
BsaBI GATnn'nnATC
Cuts at: 0 194 2325
Size: 194 2131
BsaHI Gr'CG_yC
Cuts at: 0 1346 2325
Size: 1346 979
BsaWI w'CCGG_w
Cuts at: 0 1673 2325
Size: 1673 652
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 508 538 2325
Size: 508 30 1787
Fragments arranged by size:
1787 508 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 516 1885 2038 2074 2325
Size: 516 1369 153 36 251
Fragments arranged by size:
1369 516 251 153 36
BseYI C'CCAG_C
Cuts at: 0 520 1945 2325
Size: 520 1425 380
Fragments arranged by size:
1425 520 380
BsiHKAI G_wGCw'C
Cuts at: 0 119 856 2203 2325
Size: 119 737 1347 122
Fragments arranged by size:
1347 737 122 119
BsmI GAATG_Cn'
Cuts at: 0 316 1043 2325
Size: 316 727 1282
Fragments arranged by size:
1282 727 316
BsmBI CGTCTCn'nnnn_
Cuts at: 0 587 2325
Size: 587 1738
Bsp1286I G_dGCh'C
Cuts at: 0 17 119 856 2203 2325
Size: 17 102 737 1347 122
Fragments arranged by size:
1347 737 122 102 17
BspEI T'CCGG_A
Cuts at: 0 1673 2325
Size: 1673 652
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1257 2325
Size: 1257 1068
BsrGI T'GTAC_A
Cuts at: 0 1842 2325
Size: 1842 483
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1628 2325
Size: 1628 697
BstYI r'GATC_y
Cuts at: 0 456 484 1458 1516 2325
Size: 456 28 974 58 809
Fragments arranged by size:
974 809 456 58 28
Bsu36I CC'TnA_GG
Cuts at: 0 1924 2261 2325
Size: 1924 337 64
Fragments arranged by size:
1924 337 64
BtgI C'CryG_G
Cuts at: 0 8 2122 2325
Size: 8 2114 203
Fragments arranged by size:
2114 203 8
BtsI GCAGTG_nn'
Cuts at: 0 1717 2325
Size: 1717 608
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 528 563 2325
Size: 528 35 1762
Fragments arranged by size:
1762 528 35
EaeI y'GGCC_r
Cuts at: 0 283 1335 2325
Size: 283 1052 990
Fragments arranged by size:
1052 990 283
EarI CTCTTCn'nnn_
Cuts at: 0 214 1924 2015 2325
Size: 214 1710 91 310
Fragments arranged by size:
1710 310 214 91
EcoICRI GAG'CTC
Cuts at: 0 117 2325
Size: 117 2208
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 425 606 778 1429 1775 2325
Size: 70 355 181 172 651 346 550
Fragments arranged by size:
651 550 355 346 181 172 70
EcoO109I rG'GnC_Cy
Cuts at: 0 225 1084 1949 2325
Size: 225 859 865 376
Fragments arranged by size:
865 859 376 225
EcoRI G'AATT_C
Cuts at: 0 2134 2325
Size: 2134 191
EcoRV GAT'ATC
Cuts at: 0 445 2325
Size: 445 1880
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2154 2325
Size: 2154 171
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 508 540 1609 1641 2070 2102
Size: 51 32 425 32 1069 32 429 32
Cuts at: 2102 2325
Size: 223
Fragments arranged by size:
1069 429 425 223 51 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 2221 2325
Size: 2221 104
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 420 2325
Size: 420 1905
MscI TGG'CCA
Cuts at: 0 1337 2325
Size: 1337 988
MslI CAynn'nnrTG
Cuts at: 0 650 1553 1697 2325
Size: 650 903 144 628
Fragments arranged by size:
903 650 628 144
MspA1I CmG'CkG
Cuts at: 0 10 76 1506 2145 2325
Size: 10 66 1430 639 180
Fragments arranged by size:
1430 639 180 66 10
NcoI C'CATG_G
Cuts at: 0 2122 2325
Size: 2122 203
NdeI CA'TA_TG
Cuts at: 0 1068 2325
Size: 1068 1257
NsiI A_TGCA'T
Cuts at: 0 316 2325
Size: 316 2009
NspI r_CATG'y
Cuts at: 0 1018 1038 1563 1660 2325
Size: 1018 20 525 97 665
Fragments arranged by size:
1018 665 525 97 20
PciI A'CATG_T
Cuts at: 0 1034 1559 2325
Size: 1034 525 766
Fragments arranged by size:
1034 766 525
PflMI CCAn_nnn'nTGG
Cuts at: 0 1135 1410 2172 2325
Size: 1135 275 762 153
Fragments arranged by size:
1135 762 275 153
PpuMI rG'GwC_Cy
Cuts at: 0 225 1084 1949 2325
Size: 225 859 865 376
Fragments arranged by size:
865 859 376 225
PvuII CAG'CTG
Cuts at: 0 76 1506 2145 2325
Size: 76 1430 639 180
Fragments arranged by size:
1430 639 180 76
RsrII CG'GwC_CG
Cuts at: 0 1992 2325
Size: 1992 333
SacI G_AGCT'C
Cuts at: 0 119 2325
Size: 119 2206
SacII CC_GC'GG
Cuts at: 0 11 2325
Size: 11 2314
SapI GCTCTTCn'nnn_
Cuts at: 0 214 2325
Size: 214 2111
ScaI AGT'ACT
Cuts at: 0 1791 2325
Size: 1791 534
SfcI C'TryA_G
Cuts at: 0 573 2325
Size: 573 1752
SmlI C'TyrA_G
Cuts at: 0 1535 1595 2325
Size: 1535 60 730
Fragments arranged by size:
1535 730 60
SphI G_CATG'C
Cuts at: 0 1018 2325
Size: 1018 1307
SspI AAT'ATT
Cuts at: 0 143 1002 1268 2325
Size: 143 859 266 1057
Fragments arranged by size:
1057 859 266 143
StuI AGG'CCT
Cuts at: 0 1134 2325
Size: 1134 1191
StyI C'CwwG_G
Cuts at: 0 1129 1181 1908 2006 2122 2325
Size: 1129 52 727 98 116 203
Fragments arranged by size:
1129 727 203 116 98 52
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 495 2325
Size: 495 1830
TatI w'GTAC_w
Cuts at: 0 289 1789 1842 2325
Size: 289 1500 53 483
Fragments arranged by size:
1500 483 289 53
XbaI T'CTAG_A
Cuts at: 0 1434 2325
Size: 1434 891
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2313 2325
Size: 2313 12
XhoI C'TCGA_G
Cuts at: 0 1595 2325
Size: 1595 730
XmnI GAAnn'nnTTC
Cuts at: 0 1430 1442 2325
Size: 1430 12 883
Fragments arranged by size:
1430 883 12
ZraI GAC'GTC
Cuts at: 0 1347 2325
Size: 1347 978
Enzymes that do cut:
AatII AcuI AflIII AhdI AlwNI ApaLI ApoI AvaI
BaeI BamHI BanII BbsI BbvCI BfrBI BglII BlpI
Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI BsmBI
Bsp1286I BspEI BspMI BsrGI BstAPI BstYI Bsu36I BtgI
BtsI CspCI EaeI EarI EcoICRI Eco57MI EcoO109I EcoRI
EcoRV FalI Hin4I HindIII MmeI MscI MslI MspA1I
NcoI NdeI NsiI NspI PciI PflMI PpuMI PvuII
RsrII SacI SacII SapI ScaI SfcI SmlI SphI
SspI StuI StyI TaqII TatI XbaI XcmI XhoI
XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AgeI AleI
AlfI AloI ApaI AscI AseI AsiSI AvrII BanI
BbeI BcgI BciVI BclI BglI BmrI BmtI BsaAI
BsgI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI BssHII
BssSI BstBI BstEII BstXI BstZ17I BtgZI ClaI DraI
DraIII DrdI EagI EciI EcoNI FseI FspI FspAI
HaeII HincII HpaI KasI KpnI MfeI MluI NaeI
NarI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SwaI TstI
Tth111I XmaI