Restriction map for pF1KSDA0326
 (Linear) (Six-base) MAPSORT of: KIAA0326.seq  Check: 1882  from: 1  to: 2607



KIAA0326.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     544    1112    2607 
   Size:        544     568    1495

  Fragments arranged by size: 

                1495     568     544
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     459     627    1215    1467    1932    2607 
   Size:        459     168     588     252     465     675

  Fragments arranged by size: 

                 675     588     465     459     252     168
 
AfeI AGC'GCT

Cuts at:      0     892    2607 
   Size:        892    1715
 
AflII C'TTAA_G

Cuts at:      0    1921    2124    2607 
   Size:       1921     203     483

  Fragments arranged by size: 

                1921     483     203
 
AgeI A'CCGG_T

Cuts at:      0    1357    2607 
   Size:       1357    1250
 
AleI CACnn'nnGTG

Cuts at:      0      71    1358    2594    2607 
   Size:         71    1287    1236      13

  Fragments arranged by size: 

                1287    1236      71      13
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1768    2607 
   Size:       1768     839
 
ApaI G_GGCC'C

Cuts at:      0      28    1638    2114    2607 
   Size:         28    1610     476     493

  Fragments arranged by size: 

                1610     493     476      28
 
ApaLI G'TGCA_C

Cuts at:      0     705    2607 
   Size:        705    1902
 
ApoI r'AATT_y

Cuts at:      0    1869    2607 
   Size:       1869     738
 
AvaI C'yCGr_G

Cuts at:      0       7     877    1465    1660    1879    2392    2533    2607 
   Size:          7     870     588     195     219     513     141      74

  Fragments arranged by size: 

                 870     588     513     219     195     141      74       7
            
 
AvrII C'CTAG_G

Cuts at:      0    2094    2607 
   Size:       2094     513
 
BamHI G'GATC_C

Cuts at:      0    1766    2607 
   Size:       1766     841
 
BanI G'GyrC_C

Cuts at:      0      73     500    1172    2354    2607 
   Size:         73     427     672    1182     253

  Fragments arranged by size: 

                1182     672     427     253      73
 
BanII G_rGCy'C

Cuts at:      0       8      28      55     103     398     571     655     991 
   Size:          8      20      27      48     295     173      84     336

Cuts at:    991    1327    1495    1638    1661    2114    2224    2254    2284 
   Size:        336     168     143      23     453     110      30      30

Cuts at:   2284    2607 
   Size:        323

  Fragments arranged by size: 

                 453     336     336     323     295     173     168     143
                 110      84      48      30      30      27      23      20
                   8
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1624    2607 
   Size:       1624     983
 
BbvCI CC'TCA_GC

Cuts at:      0    2173    2607 
   Size:       2173     434
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1044    1078    2607 
   Size:       1044      34    1529

  Fragments arranged by size: 

                1529    1044      34
 
BglI GCCn_nnn'nGGC

Cuts at:      0     331    2607 
   Size:        331    2276
 
Bme1580I G_kGCm'C

Cuts at:      0      28     625     709     877    1129    1213    1465    1638 
   Size:         28     597      84     168     252      84     252     173

Cuts at:   1638    2114    2533    2607 
   Size:        476     419      74

  Fragments arranged by size: 

                 597     476     419     252     252     173     168      84
                  84      74      28
 
BmgBI CAC'GTC

Cuts at:      0    1512    2607 
   Size:       1512    1095
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     247     370    1123    2607 
   Size:        247     123     753    1484

  Fragments arranged by size: 

                1484     753     247     123
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     213    2607 
   Size:        213    2394
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     669    1606    2017    2287    2390    2607 
   Size:        208     461     937     411     270     103     217

  Fragments arranged by size: 

                 937     461     411     270     217     208     103
 
Bpu10I CC'TnA_GC

Cuts at:      0    1153    2173    2607 
   Size:       1153    1020     434

  Fragments arranged by size: 

                1153    1020     434
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     901    2607 
   Size:        901    1706
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     134    2443    2607 
   Size:        134    2309     164

  Fragments arranged by size: 

                2309     164     134
 
BsaBI GATnn'nnATC

Cuts at:      0    1771    2607 
   Size:       1771     836
 
BsaWI w'CCGG_w

Cuts at:      0      42    1357    2607 
   Size:         42    1315    1250

  Fragments arranged by size: 

                1315    1250      42
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     523     553    1975    2005    2607 
   Size:        523      30    1422      30     602

  Fragments arranged by size: 

                1422     602     523      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     109     139     904    1823    2020    2607 
   Size:        109      30     765     919     197     587

  Fragments arranged by size: 

                 919     765     587     197     109      30
 
BseYI C'CCAG_C

Cuts at:      0     282     397    1001    1623    1653    2113    2241    2607 
   Size:        282     115     604     622      30     460     128     366

  Fragments arranged by size: 

                 622     604     460     366     282     128     115      30
            
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     436     601     685     942    2176    2607 
   Size:        436     165      84     257    1234     431

  Fragments arranged by size: 

                1234     436     431     257     165      84
 
BsiEI CG_ry'CG

Cuts at:      0    2337    2607 
   Size:       2337     270
 
BsiHKAI G_wGCw'C

Cuts at:      0     571     655     709     739     991    1327    1495    1549 
   Size:        571      84      54      30     252     336     168      54

Cuts at:   1549    2030    2224    2254    2607 
   Size:        481     194      30     353

  Fragments arranged by size: 

                 571     481     353     336     252     194     168      84
                  54      54      30      30
 
BsmI GAATG_Cn'

Cuts at:      0    2158    2607 
   Size:       2158     449
 
Bsp1286I G_dGCh'C

Cuts at:      0       8      28      55     103     398     571     625     655 
   Size:          8      20      27      48     295     173      54      30

Cuts at:    655     709     739     877     991    1129    1213    1327    1465 
   Size:         54      30     138     114     138      84     114     138

Cuts at:   1465    1495    1549    1638    1661    2030    2114    2224    2254 
   Size:         30      54      89      23     369      84     110      30

Cuts at:   2254    2284    2533    2607 
   Size:         30     249      74

  Fragments arranged by size: 

                 369     295     249     173     138     138     138     114
                 114     110      89      84      84      74      54      54
                  54      48      30      30      30      30      30      27
                  23      20       8
 
BspEI T'CCGG_A

Cuts at:      0      42    2607 
   Size:         42    2565
 
BspHI T'CATG_A

Cuts at:      0    1319    2607 
   Size:       1319    1288
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     544    1112    2607 
   Size:        544     568    1495

  Fragments arranged by size: 

                1495     568     544
 
BsrBI CCG'CTC

Cuts at:      0     525    1197    1281    1900    2607 
   Size:        525     672      84     619     707

  Fragments arranged by size: 

                 707     672     619     525      84
 
BsrFI r'CCGG_y

Cuts at:      0     601     769    1357    1441    2545    2607 
   Size:        601     168     588      84    1104      62

  Fragments arranged by size: 

                1104     601     588     168      84      62
 
BssSI C'ACGA_G

Cuts at:      0    1579    1714    2607 
   Size:       1579     135     893

  Fragments arranged by size: 

                1579     893     135
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     461     579     713     965    2607 
   Size:        461     118     134     252    1642

  Fragments arranged by size: 

                1642     461     252     134     118
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     255     314    2607 
   Size:        255      59    2293

  Fragments arranged by size: 

                2293     255      59
 
BstYI r'GATC_y

Cuts at:      0      30     193     266     929    1766    2607 
   Size:         30     163      73     663     837     841

  Fragments arranged by size: 

                 841     837     663     163      73      30
 
BtgI C'CryG_G

Cuts at:      0    1272    2049    2478    2607 
   Size:       1272     777     429     129

  Fragments arranged by size: 

                1272     777     429     129
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0    1794    2607 
   Size:       1794     813
 
BtsI GCAGTG_nn'

Cuts at:      0     349    1995    2607 
   Size:        349    1646     612

  Fragments arranged by size: 

                1646     612     349
 
DraI TTT'AAA

Cuts at:      0    2142    2607 
   Size:       2142     465
 
DraIII CAC_nnn'GTG

Cuts at:      0     579     713     965    1599    2607 
   Size:        579     134     252     634    1008

  Fragments arranged by size: 

                1008     634     579     252     134
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     211    2607 
   Size:        211    2396
 
EaeI y'GGCC_r

Cuts at:      0     897    1065    2607 
   Size:        897     168    1542

  Fragments arranged by size: 

                1542     897     168
 
EarI CTCTTCn'nnn_

Cuts at:      0      86     967    2212    2224    2242    2259    2607 
   Size:         86     881    1245      12      18      17     348

  Fragments arranged by size: 

                1245     881     348      86      18      17      12
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     451    1327    2607 
   Size:        451     876    1280

  Fragments arranged by size: 

                1280     876     451
 
EcoICRI GAG'CTC

Cuts at:      0     569     653     989    1325    1493    2222    2252    2607 
   Size:        569      84     336     336     168     729      30     355

  Fragments arranged by size: 

                 729     569     355     336     336     168      84      30
            
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     208     459     627     669    1215    1467    1606    1932 
   Size:        208     251     168      42     546     252     139     326

Cuts at:   1932    2017    2287    2390    2607 
   Size:         85     270     103     217

  Fragments arranged by size: 

                 546     326     270     252     251     217     208     168
                 139     103      85      42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1108    1862    2607 
   Size:       1108     754     745

  Fragments arranged by size: 

                1108     754     745
 
EcoO109I rG'GnC_Cy

Cuts at:      0      24     204    1113    1634    1665    1905    2110    2321 
   Size:         24     180     909     521      31     240     205     211

Cuts at:   2321    2397    2607 
   Size:         76     210

  Fragments arranged by size: 

                 909     521     240     211     210     205     180      76
                  31      24
 
EcoRI G'AATT_C

Cuts at:      0    1869    2607 
   Size:       1869     738
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    1874    2607 
   Size:       1874     733
 
HaeII r_GCGC'y

Cuts at:      0     353     894    1160    2607 
   Size:        353     541     266    1447

  Fragments arranged by size: 

                1447     541     353     266
 
HindIII A'AGCT_T

Cuts at:      0    2163    2607 
   Size:       2163     444
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     680    1268    1406    1520    1574    2607 
   Size:        680     588     138     114      54    1033

  Fragments arranged by size: 

                1033     680     588     138     114      54
 
MslI CAynn'nnrTG

Cuts at:      0      71    1358    1522    1750    2594    2607 
   Size:         71    1287     164     228     844      13

  Fragments arranged by size: 

                1287     844     228     164      71      13
 
MspA1I CmG'CkG

Cuts at:      0     338     647     956    1095    1274    2306    2607 
   Size:        338     309     309     139     179    1032     301

  Fragments arranged by size: 

                1032     338     309     309     301     179     139
 
NaeI GCC'GGC

Cuts at:      0    2547    2607 
   Size:       2547      60
 
NcoI C'CATG_G

Cuts at:      0    2478    2607 
   Size:       2478     129
 
NgoMIV G'CCGG_C

Cuts at:      0    2545    2607 
   Size:       2545      62
 
PasI CC'CwG_GG

Cuts at:      0    1638    2286    2607 
   Size:       1638     648     321

  Fragments arranged by size: 

                1638     648     321
 
PflMI CCAn_nnn'nTGG

Cuts at:      0    1584    2607 
   Size:       1584    1023
 
PfoI T'CCnGG_A

Cuts at:      0    1568    2012    2264    2607 
   Size:       1568     444     252     343

  Fragments arranged by size: 

                1568     444     343     252
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0    1102    1134    1522    1554    2607 
   Size:       1102      32     388      32    1053

  Fragments arranged by size: 

                1102    1053     388      32      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     204    1113    1665    2397    2607 
   Size:        204     909     552     732     210

  Fragments arranged by size: 

                 909     732     552     210     204
 
PspOMI G'GGCC_C

Cuts at:      0      24    1634    2110    2607 
   Size:         24    1610     476     497

  Fragments arranged by size: 

                1610     497     476      24
 
PstI C_TGCA'G

Cuts at:      0      38    2607 
   Size:         38    2569
 
PvuII CAG'CTG

Cuts at:      0     338     647    2607 
   Size:        338     309    1960

  Fragments arranged by size: 

                1960     338     309
 
SacI G_AGCT'C

Cuts at:      0     571     655     991    1327    1495    2224    2254    2607 
   Size:        571      84     336     336     168     729      30     353

  Fragments arranged by size: 

                 729     571     353     336     336     168      84      30
            
 
SacII CC_GC'GG

Cuts at:      0    1275    2607 
   Size:       1275    1332
 
SanDI GG'GwC_CC

Cuts at:      0    1665    2607 
   Size:       1665     942
 
SapI GCTCTTCn'nnn_

Cuts at:      0     967    2212    2242    2259    2607 
   Size:        967    1245      30      17     348

  Fragments arranged by size: 

                1245     967     348      30      17
 
SexAI A'CCwGG_T

Cuts at:      0     575     659    2607 
   Size:        575      84    1948

  Fragments arranged by size: 

                1948     575      84
 
SfcI C'TryA_G

Cuts at:      0      34    2607 
   Size:         34    2573
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0     331    2607 
   Size:        331    2276
 
SgrAI Cr'CCGG_yG

Cuts at:      0     601     769    1357    1441    2607 
   Size:        601     168     588      84    1166

  Fragments arranged by size: 

                1166     601     588     168      84
 
SmaI CCC'GGG

Cuts at:      0    1881    2607 
   Size:       1881     726
 
SmlI C'TyrA_G

Cuts at:      0     916    1921    2124    2607 
   Size:        916    1005     203     483

  Fragments arranged by size: 

                1005     916     483     203
 
StuI AGG'CCT

Cuts at:      0     325    1564    2607 
   Size:        325    1239    1043

  Fragments arranged by size: 

                1239    1043     325
 
StyI C'CwwG_G

Cuts at:      0     164     254     320    1849    2094    2478    2607 
   Size:        164      90      66    1529     245     384     129

  Fragments arranged by size: 

                1529     384     245     164     129      90      66
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     745     862    2062    2386    2606    2607 
   Size:        745     117    1200     324     220       1

  Fragments arranged by size: 

                1200     745     324     220     117       1
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     745     777    2607 
   Size:        745      32    1830

  Fragments arranged by size: 

                1830     745      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    2505    2607 
   Size:       2505     102
 
XmaI C'CCGG_G

Cuts at:      0    1879    2607 
   Size:       1879     728
 
XmnI GAAnn'nnTTC

Cuts at:      0     724     892     976    1537    1564    2607 
   Size:        724     168      84     561      27    1043

  Fragments arranged by size: 

                1043     724     561     168      84      27
 

 Enzymes that do cut:

     AarI     AcuI     AfeI    AflII     AgeI     AleI     AlfI     ApaI
    ApaLI     ApoI     AvaI    AvrII    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI     BglI Bme1580I    BmgBI     BmrI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BssSI   BstAPI    BstXI    BstYI     BtgI    BtgZI
     BtsI     DraI   DraIII     DrdI     EaeI     EarI     EciI  EcoICRI
  Eco57MI    EcoNI EcoO109I    EcoRI     FalI    HaeII  HindIII     MmeI
     MslI   MspA1I     NaeI     NcoI   NgoMIV     PasI    PflMI     PfoI
     PpiI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII    SanDI
     SapI    SexAI     SfcI     SfiI    SgrAI     SmaI     SmlI     StuI
     StyI    TaqII     TstI     XcmI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI   AflIII     AhdI     AloI    AlwNI
     AscI     AseI    AsiSI     BaeI     BbeI    BciVI     BclI    BfrBI
    BglII     BlpI     BmtI    BsaAI    BsaHI    BsiWI    BsmBI    BsrDI
    BsrGI   BssHII    BstBI   BstEII  BstZ17I   Bsu36I     ClaI    CspCI
     EagI    EcoRV     FseI     FspI    FspAI    Hin4I   HincII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     NarI     NdeI     NheI
     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI     PmlI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI     SbfI
     ScaI     SfoI     SgfI    SnaBI     SpeI     SphI     SrfI     SspI
     SwaI     TatI  Tth111I     XbaI     XhoI     ZraI
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