(Linear) (Six-base) MAPSORT of: KIAA0326.seq Check: 1882 from: 1 to: 2607 KIAA0326.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 544 1112 2607 Size: 544 568 1495 Fragments arranged by size: 1495 568 544 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 459 627 1215 1467 1932 2607 Size: 459 168 588 252 465 675 Fragments arranged by size: 675 588 465 459 252 168 AfeI AGC'GCT Cuts at: 0 892 2607 Size: 892 1715 AflII C'TTAA_G Cuts at: 0 1921 2124 2607 Size: 1921 203 483 Fragments arranged by size: 1921 483 203 AgeI A'CCGG_T Cuts at: 0 1357 2607 Size: 1357 1250 AleI CACnn'nnGTG Cuts at: 0 71 1358 2594 2607 Size: 71 1287 1236 13 Fragments arranged by size: 1287 1236 71 13 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1768 2607 Size: 1768 839 ApaI G_GGCC'C Cuts at: 0 28 1638 2114 2607 Size: 28 1610 476 493 Fragments arranged by size: 1610 493 476 28 ApaLI G'TGCA_C Cuts at: 0 705 2607 Size: 705 1902 ApoI r'AATT_y Cuts at: 0 1869 2607 Size: 1869 738 AvaI C'yCGr_G Cuts at: 0 7 877 1465 1660 1879 2392 2533 2607 Size: 7 870 588 195 219 513 141 74 Fragments arranged by size: 870 588 513 219 195 141 74 7 AvrII C'CTAG_G Cuts at: 0 2094 2607 Size: 2094 513 BamHI G'GATC_C Cuts at: 0 1766 2607 Size: 1766 841 BanI G'GyrC_C Cuts at: 0 73 500 1172 2354 2607 Size: 73 427 672 1182 253 Fragments arranged by size: 1182 672 427 253 73 BanII G_rGCy'C Cuts at: 0 8 28 55 103 398 571 655 991 Size: 8 20 27 48 295 173 84 336 Cuts at: 991 1327 1495 1638 1661 2114 2224 2254 2284 Size: 336 168 143 23 453 110 30 30 Cuts at: 2284 2607 Size: 323 Fragments arranged by size: 453 336 336 323 295 173 168 143 110 84 48 30 30 27 23 20 8 BbsI GAAGACnn'nnnn_ Cuts at: 0 1624 2607 Size: 1624 983 BbvCI CC'TCA_GC Cuts at: 0 2173 2607 Size: 2173 434 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1044 1078 2607 Size: 1044 34 1529 Fragments arranged by size: 1529 1044 34 BglI GCCn_nnn'nGGC Cuts at: 0 331 2607 Size: 331 2276 Bme1580I G_kGCm'C Cuts at: 0 28 625 709 877 1129 1213 1465 1638 Size: 28 597 84 168 252 84 252 173 Cuts at: 1638 2114 2533 2607 Size: 476 419 74 Fragments arranged by size: 597 476 419 252 252 173 168 84 84 74 28 BmgBI CAC'GTC Cuts at: 0 1512 2607 Size: 1512 1095 BmrI ACTGGGnnnn_n' Cuts at: 0 247 370 1123 2607 Size: 247 123 753 1484 Fragments arranged by size: 1484 753 247 123 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 213 2607 Size: 213 2394 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 669 1606 2017 2287 2390 2607 Size: 208 461 937 411 270 103 217 Fragments arranged by size: 937 461 411 270 217 208 103 Bpu10I CC'TnA_GC Cuts at: 0 1153 2173 2607 Size: 1153 1020 434 Fragments arranged by size: 1153 1020 434 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 901 2607 Size: 901 1706 BsaI GGTCTCn'nnnn_ Cuts at: 0 134 2443 2607 Size: 134 2309 164 Fragments arranged by size: 2309 164 134 BsaBI GATnn'nnATC Cuts at: 0 1771 2607 Size: 1771 836 BsaWI w'CCGG_w Cuts at: 0 42 1357 2607 Size: 42 1315 1250 Fragments arranged by size: 1315 1250 42 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 523 553 1975 2005 2607 Size: 523 30 1422 30 602 Fragments arranged by size: 1422 602 523 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 109 139 904 1823 2020 2607 Size: 109 30 765 919 197 587 Fragments arranged by size: 919 765 587 197 109 30 BseYI C'CCAG_C Cuts at: 0 282 397 1001 1623 1653 2113 2241 2607 Size: 282 115 604 622 30 460 128 366 Fragments arranged by size: 622 604 460 366 282 128 115 30 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 436 601 685 942 2176 2607 Size: 436 165 84 257 1234 431 Fragments arranged by size: 1234 436 431 257 165 84 BsiEI CG_ry'CG Cuts at: 0 2337 2607 Size: 2337 270 BsiHKAI G_wGCw'C Cuts at: 0 571 655 709 739 991 1327 1495 1549 Size: 571 84 54 30 252 336 168 54 Cuts at: 1549 2030 2224 2254 2607 Size: 481 194 30 353 Fragments arranged by size: 571 481 353 336 252 194 168 84 54 54 30 30 BsmI GAATG_Cn' Cuts at: 0 2158 2607 Size: 2158 449 Bsp1286I G_dGCh'C Cuts at: 0 8 28 55 103 398 571 625 655 Size: 8 20 27 48 295 173 54 30 Cuts at: 655 709 739 877 991 1129 1213 1327 1465 Size: 54 30 138 114 138 84 114 138 Cuts at: 1465 1495 1549 1638 1661 2030 2114 2224 2254 Size: 30 54 89 23 369 84 110 30 Cuts at: 2254 2284 2533 2607 Size: 30 249 74 Fragments arranged by size: 369 295 249 173 138 138 138 114 114 110 89 84 84 74 54 54 54 48 30 30 30 30 30 27 23 20 8 BspEI T'CCGG_A Cuts at: 0 42 2607 Size: 42 2565 BspHI T'CATG_A Cuts at: 0 1319 2607 Size: 1319 1288 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 544 1112 2607 Size: 544 568 1495 Fragments arranged by size: 1495 568 544 BsrBI CCG'CTC Cuts at: 0 525 1197 1281 1900 2607 Size: 525 672 84 619 707 Fragments arranged by size: 707 672 619 525 84 BsrFI r'CCGG_y Cuts at: 0 601 769 1357 1441 2545 2607 Size: 601 168 588 84 1104 62 Fragments arranged by size: 1104 601 588 168 84 62 BssSI C'ACGA_G Cuts at: 0 1579 1714 2607 Size: 1579 135 893 Fragments arranged by size: 1579 893 135 BstAPI GCAn_nnn'nTGC Cuts at: 0 461 579 713 965 2607 Size: 461 118 134 252 1642 Fragments arranged by size: 1642 461 252 134 118 BstXI CCAn_nnnn'nTGG Cuts at: 0 255 314 2607 Size: 255 59 2293 Fragments arranged by size: 2293 255 59 BstYI r'GATC_y Cuts at: 0 30 193 266 929 1766 2607 Size: 30 163 73 663 837 841 Fragments arranged by size: 841 837 663 163 73 30 BtgI C'CryG_G Cuts at: 0 1272 2049 2478 2607 Size: 1272 777 429 129 Fragments arranged by size: 1272 777 429 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1794 2607 Size: 1794 813 BtsI GCAGTG_nn' Cuts at: 0 349 1995 2607 Size: 349 1646 612 Fragments arranged by size: 1646 612 349 DraI TTT'AAA Cuts at: 0 2142 2607 Size: 2142 465 DraIII CAC_nnn'GTG Cuts at: 0 579 713 965 1599 2607 Size: 579 134 252 634 1008 Fragments arranged by size: 1008 634 579 252 134 DrdI GACnn_nn'nnGTC Cuts at: 0 211 2607 Size: 211 2396 EaeI y'GGCC_r Cuts at: 0 897 1065 2607 Size: 897 168 1542 Fragments arranged by size: 1542 897 168 EarI CTCTTCn'nnn_ Cuts at: 0 86 967 2212 2224 2242 2259 2607 Size: 86 881 1245 12 18 17 348 Fragments arranged by size: 1245 881 348 86 18 17 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 451 1327 2607 Size: 451 876 1280 Fragments arranged by size: 1280 876 451 EcoICRI GAG'CTC Cuts at: 0 569 653 989 1325 1493 2222 2252 2607 Size: 569 84 336 336 168 729 30 355 Fragments arranged by size: 729 569 355 336 336 168 84 30 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 208 459 627 669 1215 1467 1606 1932 Size: 208 251 168 42 546 252 139 326 Cuts at: 1932 2017 2287 2390 2607 Size: 85 270 103 217 Fragments arranged by size: 546 326 270 252 251 217 208 168 139 103 85 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 1108 1862 2607 Size: 1108 754 745 Fragments arranged by size: 1108 754 745 EcoO109I rG'GnC_Cy Cuts at: 0 24 204 1113 1634 1665 1905 2110 2321 Size: 24 180 909 521 31 240 205 211 Cuts at: 2321 2397 2607 Size: 76 210 Fragments arranged by size: 909 521 240 211 210 205 180 76 31 24 EcoRI G'AATT_C Cuts at: 0 1869 2607 Size: 1869 738 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1874 2607 Size: 1874 733 HaeII r_GCGC'y Cuts at: 0 353 894 1160 2607 Size: 353 541 266 1447 Fragments arranged by size: 1447 541 353 266 HindIII A'AGCT_T Cuts at: 0 2163 2607 Size: 2163 444 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 680 1268 1406 1520 1574 2607 Size: 680 588 138 114 54 1033 Fragments arranged by size: 1033 680 588 138 114 54 MslI CAynn'nnrTG Cuts at: 0 71 1358 1522 1750 2594 2607 Size: 71 1287 164 228 844 13 Fragments arranged by size: 1287 844 228 164 71 13 MspA1I CmG'CkG Cuts at: 0 338 647 956 1095 1274 2306 2607 Size: 338 309 309 139 179 1032 301 Fragments arranged by size: 1032 338 309 309 301 179 139 NaeI GCC'GGC Cuts at: 0 2547 2607 Size: 2547 60 NcoI C'CATG_G Cuts at: 0 2478 2607 Size: 2478 129 NgoMIV G'CCGG_C Cuts at: 0 2545 2607 Size: 2545 62 PasI CC'CwG_GG Cuts at: 0 1638 2286 2607 Size: 1638 648 321 Fragments arranged by size: 1638 648 321 PflMI CCAn_nnn'nTGG Cuts at: 0 1584 2607 Size: 1584 1023 PfoI T'CCnGG_A Cuts at: 0 1568 2012 2264 2607 Size: 1568 444 252 343 Fragments arranged by size: 1568 444 343 252 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1102 1134 1522 1554 2607 Size: 1102 32 388 32 1053 Fragments arranged by size: 1102 1053 388 32 32 PpuMI rG'GwC_Cy Cuts at: 0 204 1113 1665 2397 2607 Size: 204 909 552 732 210 Fragments arranged by size: 909 732 552 210 204 PspOMI G'GGCC_C Cuts at: 0 24 1634 2110 2607 Size: 24 1610 476 497 Fragments arranged by size: 1610 497 476 24 PstI C_TGCA'G Cuts at: 0 38 2607 Size: 38 2569 PvuII CAG'CTG Cuts at: 0 338 647 2607 Size: 338 309 1960 Fragments arranged by size: 1960 338 309 SacI G_AGCT'C Cuts at: 0 571 655 991 1327 1495 2224 2254 2607 Size: 571 84 336 336 168 729 30 353 Fragments arranged by size: 729 571 353 336 336 168 84 30 SacII CC_GC'GG Cuts at: 0 1275 2607 Size: 1275 1332 SanDI GG'GwC_CC Cuts at: 0 1665 2607 Size: 1665 942 SapI GCTCTTCn'nnn_ Cuts at: 0 967 2212 2242 2259 2607 Size: 967 1245 30 17 348 Fragments arranged by size: 1245 967 348 30 17 SexAI A'CCwGG_T Cuts at: 0 575 659 2607 Size: 575 84 1948 Fragments arranged by size: 1948 575 84 SfcI C'TryA_G Cuts at: 0 34 2607 Size: 34 2573 SfiI GGCCn_nnn'nGGCC Cuts at: 0 331 2607 Size: 331 2276 SgrAI Cr'CCGG_yG Cuts at: 0 601 769 1357 1441 2607 Size: 601 168 588 84 1166 Fragments arranged by size: 1166 601 588 168 84 SmaI CCC'GGG Cuts at: 0 1881 2607 Size: 1881 726 SmlI C'TyrA_G Cuts at: 0 916 1921 2124 2607 Size: 916 1005 203 483 Fragments arranged by size: 1005 916 483 203 StuI AGG'CCT Cuts at: 0 325 1564 2607 Size: 325 1239 1043 Fragments arranged by size: 1239 1043 325 StyI C'CwwG_G Cuts at: 0 164 254 320 1849 2094 2478 2607 Size: 164 90 66 1529 245 384 129 Fragments arranged by size: 1529 384 245 164 129 90 66 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 745 862 2062 2386 2606 2607 Size: 745 117 1200 324 220 1 Fragments arranged by size: 1200 745 324 220 117 1 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 745 777 2607 Size: 745 32 1830 Fragments arranged by size: 1830 745 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 2505 2607 Size: 2505 102 XmaI C'CCGG_G Cuts at: 0 1879 2607 Size: 1879 728 XmnI GAAnn'nnTTC Cuts at: 0 724 892 976 1537 1564 2607 Size: 724 168 84 561 27 1043 Fragments arranged by size: 1043 724 561 168 84 27 Enzymes that do cut: AarI AcuI AfeI AflII AgeI AleI AlfI ApaI ApaLI ApoI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BglI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI HaeII HindIII MmeI MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SexAI SfcI SfiI SgrAI SmaI SmlI StuI StyI TaqII TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflIII AhdI AloI AlwNI AscI AseI AsiSI BaeI BbeI BciVI BclI BfrBI BglII BlpI BmtI BsaAI BsaHI BsiWI BsmBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI EagI EcoRV FseI FspI FspAI Hin4I HincII HpaI KasI KpnI MfeI MluI MscI NarI NdeI NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI SbfI ScaI SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI TatI Tth111I XbaI XhoI ZraI