(Linear) (Six-base) MAPSORT of: KIAA0278.seq Check: 2683 from: 1 to: 1188 KIAA0278.orf With 182 enzymes: SgfI * October 24, 2006 15:12 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 51 152 156 678 1188 Size: 51 101 4 522 510 Fragments arranged by size: 522 510 101 51 4 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 202 1188 Size: 202 986 AfeI AGC'GCT Cuts at: 0 256 319 373 1188 Size: 256 63 54 815 Fragments arranged by size: 815 256 63 54 AflIII A'CryG_T Cuts at: 0 341 1188 Size: 341 847 AgeI A'CCGG_T Cuts at: 0 194 1188 Size: 194 994 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1078 1188 Size: 1078 110 AlwNI CAG_nnn'CTG Cuts at: 0 538 903 1188 Size: 538 365 285 Fragments arranged by size: 538 365 285 AvaI C'yCGr_G Cuts at: 0 43 463 550 650 839 1183 1188 Size: 43 420 87 100 189 344 5 Fragments arranged by size: 420 344 189 100 87 43 5 BanI G'GyrC_C Cuts at: 0 140 979 1188 Size: 140 839 209 Fragments arranged by size: 839 209 140 BanII G_rGCy'C Cuts at: 0 32 563 778 1188 Size: 32 531 215 410 Fragments arranged by size: 531 410 215 32 BglI GCCn_nnn'nGGC Cuts at: 0 1093 1188 Size: 1093 95 BglII A'GATC_T Cuts at: 0 635 1188 Size: 635 553 Bme1580I G_kGCm'C Cuts at: 0 239 832 982 1188 Size: 239 593 150 206 Fragments arranged by size: 593 239 206 150 BmrI ACTGGGnnnn_n' Cuts at: 0 798 1188 Size: 798 390 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 232 334 388 409 1054 1096 1188 Size: 136 96 102 54 21 645 42 92 Fragments arranged by size: 645 136 102 96 92 54 42 21 Bpu10I CC'TnA_GC Cuts at: 0 661 1188 Size: 661 527 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 982 1188 Size: 982 206 BsaI GGTCTCn'nnnn_ Cuts at: 0 292 1123 1188 Size: 292 831 65 Fragments arranged by size: 831 292 65 BsaAI yAC'GTr Cuts at: 0 123 234 342 939 972 1188 Size: 123 111 108 597 33 216 Fragments arranged by size: 597 216 123 111 108 33 BsaWI w'CCGG_w Cuts at: 0 14 194 1172 1188 Size: 14 180 978 16 Fragments arranged by size: 978 180 16 14 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 688 718 967 970 1188 Size: 688 30 249 3 218 Fragments arranged by size: 688 249 218 30 3 BseYI C'CCAG_C Cuts at: 0 320 374 531 572 613 1178 1188 Size: 320 54 157 41 41 565 10 Fragments arranged by size: 565 320 157 54 41 41 10 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 175 1084 1188 Size: 175 909 104 Fragments arranged by size: 909 175 104 BsiEI CG_ry'CG Cuts at: 0 199 203 384 1088 1097 1188 Size: 199 4 181 704 9 91 Fragments arranged by size: 704 199 181 91 9 4 BsiHKAI G_wGCw'C Cuts at: 0 122 1188 Size: 122 1066 Bsp1286I G_dGCh'C Cuts at: 0 32 122 239 563 778 832 982 1188 Size: 32 90 117 324 215 54 150 206 Fragments arranged by size: 324 215 206 150 117 90 54 32 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 51 152 156 678 875 1188 Size: 51 101 4 522 197 313 Fragments arranged by size: 522 313 197 101 51 4 BsrFI r'CCGG_y Cuts at: 0 137 194 744 1092 1096 1188 Size: 137 57 550 348 4 92 Fragments arranged by size: 550 348 137 92 57 4 BssSI C'ACGA_G Cuts at: 0 240 478 1188 Size: 240 238 710 Fragments arranged by size: 710 240 238 BstXI CCAn_nnnn'nTGG Cuts at: 0 168 1032 1188 Size: 168 864 156 Fragments arranged by size: 864 168 156 BstYI r'GATC_y Cuts at: 0 635 722 1188 Size: 635 87 466 Fragments arranged by size: 635 466 87 BtgI C'CryG_G Cuts at: 0 49 395 446 586 1160 1188 Size: 49 346 51 140 574 28 Fragments arranged by size: 574 346 140 51 49 28 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 288 1188 Size: 288 900 DrdI GACnn_nn'nnGTC Cuts at: 0 502 1188 Size: 502 686 EaeI y'GGCC_r Cuts at: 0 65 149 746 1085 1094 1163 1188 Size: 65 84 597 339 9 69 25 Fragments arranged by size: 597 339 84 69 65 25 9 EagI C'GGCC_G Cuts at: 0 1085 1094 1188 Size: 1085 9 94 Fragments arranged by size: 1085 94 9 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 144 1141 1188 Size: 144 997 47 Fragments arranged by size: 997 144 47 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 136 202 232 334 388 409 1054 1096 Size: 136 66 30 102 54 21 645 42 Cuts at: 1096 1188 Size: 92 Fragments arranged by size: 645 136 102 92 66 54 42 30 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1078 1188 Size: 1078 110 EcoO109I rG'GnC_Cy Cuts at: 0 460 591 645 918 1188 Size: 460 131 54 273 270 Fragments arranged by size: 460 273 270 131 54 FseI GG_CCGG'CC Cuts at: 0 748 1100 1188 Size: 748 352 88 Fragments arranged by size: 748 352 88 HaeII r_GCGC'y Cuts at: 0 258 321 375 1188 Size: 258 63 54 813 Fragments arranged by size: 813 258 63 54 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 714 746 833 865 1188 Size: 714 32 87 32 323 Fragments arranged by size: 714 323 87 32 32 MscI TGG'CCA Cuts at: 0 67 1165 1188 Size: 67 1098 23 Fragments arranged by size: 1098 67 23 MslI CAynn'nnrTG Cuts at: 0 1060 1188 Size: 1060 128 MspA1I CmG'CkG Cuts at: 0 23 51 290 538 855 891 1188 Size: 23 28 239 248 317 36 297 Fragments arranged by size: 317 297 248 239 36 28 23 NaeI GCC'GGC Cuts at: 0 746 1094 1098 1188 Size: 746 348 4 90 Fragments arranged by size: 746 348 90 4 NgoMIV G'CCGG_C Cuts at: 0 744 1092 1096 1188 Size: 744 348 4 92 Fragments arranged by size: 744 348 92 4 PflMI CCAn_nnn'nTGG Cuts at: 0 538 755 972 1188 Size: 538 217 217 216 Fragments arranged by size: 538 217 217 216 PmlI CAC'GTG Cuts at: 0 123 342 1188 Size: 123 219 846 Fragments arranged by size: 846 219 123 PpuMI rG'GwC_Cy Cuts at: 0 460 591 645 918 1188 Size: 460 131 54 273 270 Fragments arranged by size: 460 273 270 131 54 PstI C_TGCA'G Cuts at: 0 89 818 989 1188 Size: 89 729 171 199 Fragments arranged by size: 729 199 171 89 SacII CC_GC'GG Cuts at: 0 52 1188 Size: 52 1136 SanDI GG'GwC_CC Cuts at: 0 460 591 1188 Size: 460 131 597 Fragments arranged by size: 597 460 131 ScaI AGT'ACT Cuts at: 0 679 709 1188 Size: 679 30 479 Fragments arranged by size: 679 479 30 SfcI C'TryA_G Cuts at: 0 85 814 985 1188 Size: 85 729 171 203 Fragments arranged by size: 729 203 171 85 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1093 1188 Size: 1093 95 SmaI CCC'GGG Cuts at: 0 45 552 1188 Size: 45 507 636 Fragments arranged by size: 636 507 45 SmlI C'TyrA_G Cuts at: 0 650 997 1188 Size: 650 347 191 Fragments arranged by size: 650 347 191 SrfI GCCC'GGGC Cuts at: 0 552 1188 Size: 552 636 StuI AGG'CCT Cuts at: 0 277 1188 Size: 277 911 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 189 780 1188 Size: 189 591 408 Fragments arranged by size: 591 408 189 TatI w'GTAC_w Cuts at: 0 677 707 818 1188 Size: 677 30 111 370 Fragments arranged by size: 677 370 111 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 427 459 1188 Size: 427 32 729 Fragments arranged by size: 729 427 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 309 621 1188 Size: 309 312 567 Fragments arranged by size: 567 312 309 XhoI C'TCGA_G Cuts at: 0 650 1188 Size: 650 538 XmaI C'CCGG_G Cuts at: 0 43 550 1188 Size: 43 507 638 Fragments arranged by size: 638 507 43 XmnI GAAnn'nnTTC Cuts at: 0 808 1188 Size: 808 380 Enzymes that do cut: AarI AcuI AfeI AflIII AgeI AlfI AlwNI AvaI BanI BanII BglI BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BsrFI BssSI BstXI BstYI BtgI BtgZI DrdI EaeI EagI EciI Eco57MI EcoNI EcoO109I FseI HaeII Hin4I MscI MslI MspA1I NaeI NgoMIV PflMI PmlI PpuMI PstI SacII SanDI ScaI SfcI SfiI SmaI SmlI SrfI StuI TaqII TatI TstI XcmI XhoI XmaI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AhdI AleI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BplI BsaBI BsaHI BsaXI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstAPI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EarI EcoICRI EcoRI EcoRV FalI FspI FspAI HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SspI StyI SwaI Tth111I XbaI ZraI