Restriction map for pF1KSDA0278
 (Linear) (Six-base) MAPSORT of: KIAA0278.seq  Check: 2683  from: 1  to: 1188



KIAA0278.orf

 With 182 enzymes: SgfI * 

                        October 24, 2006 15:12  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0      51     152     156     678    1188 
   Size:         51     101       4     522     510

  Fragments arranged by size: 

                 522     510     101      51       4
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     202    1188 
   Size:        202     986
 
AfeI AGC'GCT

Cuts at:      0     256     319     373    1188 
   Size:        256      63      54     815

  Fragments arranged by size: 

                 815     256      63      54
 
AflIII A'CryG_T

Cuts at:      0     341    1188 
   Size:        341     847
 
AgeI A'CCGG_T

Cuts at:      0     194    1188 
   Size:        194     994
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1078    1188 
   Size:       1078     110
 
AlwNI CAG_nnn'CTG

Cuts at:      0     538     903    1188 
   Size:        538     365     285

  Fragments arranged by size: 

                 538     365     285
 
AvaI C'yCGr_G

Cuts at:      0      43     463     550     650     839    1183    1188 
   Size:         43     420      87     100     189     344       5

  Fragments arranged by size: 

                 420     344     189     100      87      43       5
 
BanI G'GyrC_C

Cuts at:      0     140     979    1188 
   Size:        140     839     209

  Fragments arranged by size: 

                 839     209     140
 
BanII G_rGCy'C

Cuts at:      0      32     563     778    1188 
   Size:         32     531     215     410

  Fragments arranged by size: 

                 531     410     215      32
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1093    1188 
   Size:       1093      95
 
BglII A'GATC_T

Cuts at:      0     635    1188 
   Size:        635     553
 
Bme1580I G_kGCm'C

Cuts at:      0     239     832     982    1188 
   Size:        239     593     150     206

  Fragments arranged by size: 

                 593     239     206     150
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     798    1188 
   Size:        798     390
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     232     334     388     409    1054    1096    1188 
   Size:        136      96     102      54      21     645      42      92

  Fragments arranged by size: 

                 645     136     102      96      92      54      42      21
            
 
Bpu10I CC'TnA_GC

Cuts at:      0     661    1188 
   Size:        661     527
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     982    1188 
   Size:        982     206
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     292    1123    1188 
   Size:        292     831      65

  Fragments arranged by size: 

                 831     292      65
 
BsaAI yAC'GTr

Cuts at:      0     123     234     342     939     972    1188 
   Size:        123     111     108     597      33     216

  Fragments arranged by size: 

                 597     216     123     111     108      33
 
BsaWI w'CCGG_w

Cuts at:      0      14     194    1172    1188 
   Size:         14     180     978      16

  Fragments arranged by size: 

                 978     180      16      14
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     688     718     967     970    1188 
   Size:        688      30     249       3     218

  Fragments arranged by size: 

                 688     249     218      30       3
 
BseYI C'CCAG_C

Cuts at:      0     320     374     531     572     613    1178    1188 
   Size:        320      54     157      41      41     565      10

  Fragments arranged by size: 

                 565     320     157      54      41      41      10
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     175    1084    1188 
   Size:        175     909     104

  Fragments arranged by size: 

                 909     175     104
 
BsiEI CG_ry'CG

Cuts at:      0     199     203     384    1088    1097    1188 
   Size:        199       4     181     704       9      91

  Fragments arranged by size: 

                 704     199     181      91       9       4
 
BsiHKAI G_wGCw'C

Cuts at:      0     122    1188 
   Size:        122    1066
 
Bsp1286I G_dGCh'C

Cuts at:      0      32     122     239     563     778     832     982    1188 
   Size:         32      90     117     324     215      54     150     206

  Fragments arranged by size: 

                 324     215     206     150     117      90      54      32
            
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      51     152     156     678     875    1188 
   Size:         51     101       4     522     197     313

  Fragments arranged by size: 

                 522     313     197     101      51       4
 
BsrFI r'CCGG_y

Cuts at:      0     137     194     744    1092    1096    1188 
   Size:        137      57     550     348       4      92

  Fragments arranged by size: 

                 550     348     137      92      57       4
 
BssSI C'ACGA_G

Cuts at:      0     240     478    1188 
   Size:        240     238     710

  Fragments arranged by size: 

                 710     240     238
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168    1032    1188 
   Size:        168     864     156

  Fragments arranged by size: 

                 864     168     156
 
BstYI r'GATC_y

Cuts at:      0     635     722    1188 
   Size:        635      87     466

  Fragments arranged by size: 

                 635     466      87
 
BtgI C'CryG_G

Cuts at:      0      49     395     446     586    1160    1188 
   Size:         49     346      51     140     574      28

  Fragments arranged by size: 

                 574     346     140      51      49      28
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     288    1188 
   Size:        288     900
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     502    1188 
   Size:        502     686
 
EaeI y'GGCC_r

Cuts at:      0      65     149     746    1085    1094    1163    1188 
   Size:         65      84     597     339       9      69      25

  Fragments arranged by size: 

                 597     339      84      69      65      25       9
 
EagI C'GGCC_G

Cuts at:      0    1085    1094    1188 
   Size:       1085       9      94

  Fragments arranged by size: 

                1085      94       9
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     144    1141    1188 
   Size:        144     997      47

  Fragments arranged by size: 

                 997     144      47
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     136     202     232     334     388     409    1054    1096 
   Size:        136      66      30     102      54      21     645      42

Cuts at:   1096    1188 
   Size:         92

  Fragments arranged by size: 

                 645     136     102      92      66      54      42      30
                  21
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0    1078    1188 
   Size:       1078     110
 
EcoO109I rG'GnC_Cy

Cuts at:      0     460     591     645     918    1188 
   Size:        460     131      54     273     270

  Fragments arranged by size: 

                 460     273     270     131      54
 
FseI GG_CCGG'CC

Cuts at:      0     748    1100    1188 
   Size:        748     352      88

  Fragments arranged by size: 

                 748     352      88
 
HaeII r_GCGC'y

Cuts at:      0     258     321     375    1188 
   Size:        258      63      54     813

  Fragments arranged by size: 

                 813     258      63      54
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     714     746     833     865    1188 
   Size:        714      32      87      32     323

  Fragments arranged by size: 

                 714     323      87      32      32
 
MscI TGG'CCA

Cuts at:      0      67    1165    1188 
   Size:         67    1098      23

  Fragments arranged by size: 

                1098      67      23
 
MslI CAynn'nnrTG

Cuts at:      0    1060    1188 
   Size:       1060     128
 
MspA1I CmG'CkG

Cuts at:      0      23      51     290     538     855     891    1188 
   Size:         23      28     239     248     317      36     297

  Fragments arranged by size: 

                 317     297     248     239      36      28      23
 
NaeI GCC'GGC

Cuts at:      0     746    1094    1098    1188 
   Size:        746     348       4      90

  Fragments arranged by size: 

                 746     348      90       4
 
NgoMIV G'CCGG_C

Cuts at:      0     744    1092    1096    1188 
   Size:        744     348       4      92

  Fragments arranged by size: 

                 744     348      92       4
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     538     755     972    1188 
   Size:        538     217     217     216

  Fragments arranged by size: 

                 538     217     217     216
 
PmlI CAC'GTG

Cuts at:      0     123     342    1188 
   Size:        123     219     846

  Fragments arranged by size: 

                 846     219     123
 
PpuMI rG'GwC_Cy

Cuts at:      0     460     591     645     918    1188 
   Size:        460     131      54     273     270

  Fragments arranged by size: 

                 460     273     270     131      54
 
PstI C_TGCA'G

Cuts at:      0      89     818     989    1188 
   Size:         89     729     171     199

  Fragments arranged by size: 

                 729     199     171      89
 
SacII CC_GC'GG

Cuts at:      0      52    1188 
   Size:         52    1136
 
SanDI GG'GwC_CC

Cuts at:      0     460     591    1188 
   Size:        460     131     597

  Fragments arranged by size: 

                 597     460     131
 
ScaI AGT'ACT

Cuts at:      0     679     709    1188 
   Size:        679      30     479

  Fragments arranged by size: 

                 679     479      30
 
SfcI C'TryA_G

Cuts at:      0      85     814     985    1188 
   Size:         85     729     171     203

  Fragments arranged by size: 

                 729     203     171      85
 
SfiI GGCCn_nnn'nGGCC

Cuts at:      0    1093    1188 
   Size:       1093      95
 
SmaI CCC'GGG

Cuts at:      0      45     552    1188 
   Size:         45     507     636

  Fragments arranged by size: 

                 636     507      45
 
SmlI C'TyrA_G

Cuts at:      0     650     997    1188 
   Size:        650     347     191

  Fragments arranged by size: 

                 650     347     191
 
SrfI GCCC'GGGC

Cuts at:      0     552    1188 
   Size:        552     636
 
StuI AGG'CCT

Cuts at:      0     277    1188 
   Size:        277     911
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     189     780    1188 
   Size:        189     591     408

  Fragments arranged by size: 

                 591     408     189
 
TatI w'GTAC_w

Cuts at:      0     677     707     818    1188 
   Size:        677      30     111     370

  Fragments arranged by size: 

                 677     370     111      30
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     427     459    1188 
   Size:        427      32     729

  Fragments arranged by size: 

                 729     427      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     309     621    1188 
   Size:        309     312     567

  Fragments arranged by size: 

                 567     312     309
 
XhoI C'TCGA_G

Cuts at:      0     650    1188 
   Size:        650     538
 
XmaI C'CCGG_G

Cuts at:      0      43     550    1188 
   Size:         43     507     638

  Fragments arranged by size: 

                 638     507      43
 
XmnI GAAnn'nnTTC

Cuts at:      0     808    1188 
   Size:        808     380
 

 Enzymes that do cut:

     AarI     AcuI     AfeI   AflIII     AgeI     AlfI    AlwNI     AvaI
     BanI    BanII     BglI    BglII Bme1580I     BmrI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaWI    BseRI    BseYI     BsgI    BsiEI
  BsiHKAI Bsp1286I    BspMI    BsrFI    BssSI    BstXI    BstYI     BtgI
    BtgZI     DrdI     EaeI     EagI     EciI  Eco57MI    EcoNI EcoO109I
     FseI    HaeII    Hin4I     MscI     MslI   MspA1I     NaeI   NgoMIV
    PflMI     PmlI    PpuMI     PstI    SacII    SanDI     ScaI     SfcI
     SfiI     SmaI     SmlI     SrfI     StuI    TaqII     TatI     TstI
     XcmI     XhoI     XmaI     XmnI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI    AflII     AhdI     AleI     AloI
     ApaI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BlpI    BmgBI     BmtI     BplI    BsaBI    BsaHI    BsaXI    BsiWI
     BsmI    BsmBI    BspEI    BspHI    BsrBI    BsrDI    BsrGI   BssHII
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtsI     ClaI    CspCI
     DraI   DraIII     EarI  EcoICRI    EcoRI    EcoRV     FalI     FspI
    FspAI   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     NarI     NcoI     NdeI     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PfoI     PmeI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
     SalI     SapI     SbfI    SexAI     SfoI     SgfI    SgrAI    SnaBI
     SpeI     SphI     SspI     StyI     SwaI  Tth111I     XbaI     ZraI
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