Restriction map for
pF1KA0162
(Linear) (Six-base) MAPSORT of: KIAA0162.seq Check: 8639 from: 1 to: 5178
KIAA0162.orf
With 182 enzymes: SgfI *
January 9, 2009 11:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1800 2277 4605 5178
Size: 1800 477 2328 573
Fragments arranged by size:
2328 1800 573 477
AccI GT'mk_AC
Cuts at: 0 1388 5178
Size: 1388 3790
Acc65I G'GTAC_C
Cuts at: 0 66 4484 5178
Size: 66 4418 694
Fragments arranged by size:
4418 694 66
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 944 1026 1486 1997 2225 3022 3031 3935
Size: 944 82 460 511 228 797 9 904
Cuts at: 3935 4158 5178
Size: 223 1020
Fragments arranged by size:
1020 944 904 797 511 460 228 223
82 9
AflIII A'CryG_T
Cuts at: 0 1565 5178
Size: 1565 3613
AhdI GACnn_n'nnGTC
Cuts at: 0 2737 5178
Size: 2737 2441
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 4417 5178
Size: 4417 761
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 3770 3802 5178
Size: 3770 32 1376
Fragments arranged by size:
3770 1376 32
AlwNI CAG_nnn'CTG
Cuts at: 0 576 1747 1815 2628 5043 5085 5178
Size: 576 1171 68 813 2415 42 93
Fragments arranged by size:
2415 1171 813 576 93 68 42
ApaI G_GGCC'C
Cuts at: 0 1058 5178
Size: 1058 4120
ApoI r'AATT_y
Cuts at: 0 85 318 673 2512 2776 2881 3727 4210
Size: 85 233 355 1839 264 105 846 483
Cuts at: 4210 5178
Size: 968
Fragments arranged by size:
1839 968 846 483 355 264 233 105
85
AvaI C'yCGr_G
Cuts at: 0 71 1627 3031 4268 4433 5178
Size: 71 1556 1404 1237 165 745
Fragments arranged by size:
1556 1404 1237 745 165 71
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 334 367 5178
Size: 334 33 4811
Fragments arranged by size:
4811 334 33
BamHI G'GATC_C
Cuts at: 0 1220 4548 5178
Size: 1220 3328 630
Fragments arranged by size:
3328 1220 630
BanI G'GyrC_C
Cuts at: 0 66 1116 1759 2265 4484 5178
Size: 66 1050 643 506 2219 694
Fragments arranged by size:
2219 1050 694 643 506 66
BanII G_rGCy'C
Cuts at: 0 821 1058 1326 1544 1697 1799 1991 3264
Size: 821 237 268 218 153 102 192 1273
Cuts at: 3264 5178
Size: 1914
Fragments arranged by size:
1914 1273 821 268 237 218 192 153
102
BbeI G_GCGC'C
Cuts at: 0 1763 5178
Size: 1763 3415
BbsI GAAGACnn'nnnn_
Cuts at: 0 775 1985 2810 3072 5178
Size: 775 1210 825 262 2106
Fragments arranged by size:
2106 1210 825 775 262
BbvCI CC'TCA_GC
Cuts at: 0 26 740 1816 5178
Size: 26 714 1076 3362
Fragments arranged by size:
3362 1076 714 26
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1537 1571 5178
Size: 1537 34 3607
Fragments arranged by size:
3607 1537 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 604 4417 5178
Size: 604 3813 761
Fragments arranged by size:
3813 761 604
BclI T'GATC_A
Cuts at: 0 138 5178
Size: 138 5040
BglI GCCn_nnn'nGGC
Cuts at: 0 487 496 4750 4766 5178
Size: 487 9 4254 16 412
Fragments arranged by size:
4254 487 412 16 9
BglII A'GATC_T
Cuts at: 0 1280 3443 4496 4638 5178
Size: 1280 2163 1053 142 540
Fragments arranged by size:
2163 1280 1053 540 142
BlpI GC'TnA_GC
Cuts at: 0 3388 5178
Size: 3388 1790
Bme1580I G_kGCm'C
Cuts at: 0 1058 3595 3664 5178
Size: 1058 2537 69 1514
Fragments arranged by size:
2537 1514 1058 69
BmrI ACTGGGnnnn_n'
Cuts at: 0 815 904 4429 4740 5028 5178
Size: 815 89 3525 311 288 150
Fragments arranged by size:
3525 815 311 288 150 89
BmtI G_CTAG'C
Cuts at: 0 1454 5178
Size: 1454 3724
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 488 727 979 1724 2705 3352 4454
Size: 307 181 239 252 745 981 647 1102
Cuts at: 4454 4740 4897 5178
Size: 286 157 281
Fragments arranged by size:
1102 981 745 647 307 286 281 252
239 181 157
Bpu10I CC'TnA_GC
Cuts at: 0 26 740 1538 1816 5178
Size: 26 714 798 278 3362
Fragments arranged by size:
3362 798 714 278 26
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1336 1528 1945 2170 2474 2803 3088 5178
Size: 1336 192 417 225 304 329 285 2090
Fragments arranged by size:
2090 1336 417 329 304 285 225 192
BsaI GGTCTCn'nnnn_
Cuts at: 0 3469 4036 5178
Size: 3469 567 1142
Fragments arranged by size:
3469 1142 567
BsaAI yAC'GTr
Cuts at: 0 4806 5178
Size: 4806 372
BsaBI GATnn'nnATC
Cuts at: 0 3248 5178
Size: 3248 1930
BsaHI Gr'CG_yC
Cuts at: 0 1760 2567 3531 5178
Size: 1760 807 964 1647
Fragments arranged by size:
1760 1647 964 807
BsaWI w'CCGG_w
Cuts at: 0 3410 4481 5178
Size: 3410 1071 697
Fragments arranged by size:
3410 1071 697
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 1144 1174 3103 3133 4452 4482
Size: 700 30 414 30 1929 30 1319 30
Cuts at: 4482 5178
Size: 696
Fragments arranged by size:
1929 1319 700 696 414 30 30 30
30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 43 112 127 130 133 136 139 217
Size: 43 69 15 3 3 3 3 78
Cuts at: 217 328 409 491 523 526 544 1537 1981
Size: 111 81 82 32 3 18 993 444
Cuts at: 1981 2074 2349 2869 3454 3952 4168 4345 5178
Size: 93 275 520 585 498 216 177 833
Fragments arranged by size:
993 833 585 520 498 444 275 216
177 111 93 82 81 78 69 43
32 18 15 3 3 3 3 3
BseYI C'CCAG_C
Cuts at: 0 1057 1211 1588 3044 4617 4740 4823 4946
Size: 1057 154 377 1456 1573 123 83 123
Cuts at: 4946 4952 5104 5178
Size: 6 152 74
Fragments arranged by size:
1573 1456 1057 377 154 152 123 123
83 74 6
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2125 2188 2811 3848 5178
Size: 2125 63 623 1037 1330
Fragments arranged by size:
2125 1330 1037 623 63
BsiHKAI G_wGCw'C
Cuts at: 0 1544 5178
Size: 1544 3634
BsmI GAATG_Cn'
Cuts at: 0 972 2333 4662 5178
Size: 972 1361 2329 516
Fragments arranged by size:
2329 1361 972 516
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2745 5178
Size: 2745 2433
Bsp1286I G_dGCh'C
Cuts at: 0 821 1058 1326 1544 1697 1799 1991 3264
Size: 821 237 268 218 153 102 192 1273
Cuts at: 3264 3595 3664 5178
Size: 331 69 1514
Fragments arranged by size:
1514 1273 821 331 268 237 218 192
153 102 69
BspEI T'CCGG_A
Cuts at: 0 3410 5178
Size: 3410 1768
BspHI T'CATG_A
Cuts at: 0 3083 3800 5178
Size: 3083 717 1378
Fragments arranged by size:
3083 1378 717
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 887 1655 1800 2277 3847 4605 5178
Size: 887 768 145 477 1570 758 573
Fragments arranged by size:
1570 887 768 758 573 477 145
BsrBI CCG'CTC
Cuts at: 0 902 2475 3425 5178
Size: 902 1573 950 1753
Fragments arranged by size:
1753 1573 950 902
BsrDI GCAATG_nn'
Cuts at: 0 2348 2927 3513 3518 3573 4697 5135 5178
Size: 2348 579 586 5 55 1124 438 43
Fragments arranged by size:
2348 1124 586 579 438 55 43 5
BsrFI r'CCGG_y
Cuts at: 0 353 2117 3709 5178
Size: 353 1764 1592 1469
Fragments arranged by size:
1764 1592 1469 353
BsrGI T'GTAC_A
Cuts at: 0 1568 2601 5178
Size: 1568 1033 2577
Fragments arranged by size:
2577 1568 1033
BssSI C'ACGA_G
Cuts at: 0 1669 5178
Size: 1669 3509
BstAPI GCAn_nnn'nTGC
Cuts at: 0 3214 4264 5043 5178
Size: 3214 1050 779 135
Fragments arranged by size:
3214 1050 779 135
BstBI TT'CG_AA
Cuts at: 0 4214 5178
Size: 4214 964
BstEII G'GTnAC_C
Cuts at: 0 3051 5178
Size: 3051 2127
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1341 1588 4748 5178
Size: 1341 247 3160 430
Fragments arranged by size:
3160 1341 430 247
BstYI r'GATC_y
Cuts at: 0 956 1220 1280 3005 3443 4496 4548 4638
Size: 956 264 60 1725 438 1053 52 90
Cuts at: 4638 5178
Size: 540
Fragments arranged by size:
1725 1053 956 540 438 264 90 60
52
BtgI C'CryG_G
Cuts at: 0 482 494 1688 2129 4046 4253 5178
Size: 482 12 1194 441 1917 207 925
Fragments arranged by size:
1917 1194 925 482 441 207 12
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 5159 5178
Size: 5159 19
BtsI GCAGTG_nn'
Cuts at: 0 222 1198 3215 3595 3789 4785 4800 5044
Size: 222 976 2017 380 194 996 15 244
Cuts at: 5044 5178
Size: 134
Fragments arranged by size:
2017 996 976 380 244 222 194 134
15
DraI TTT'AAA
Cuts at: 0 1919 5178
Size: 1919 3259
DraIII CAC_nnn'GTG
Cuts at: 0 2103 2379 3059 4193 5178
Size: 2103 276 680 1134 985
Fragments arranged by size:
2103 1134 985 680 276
DrdI GACnn_nn'nnGTC
Cuts at: 0 3703 5178
Size: 3703 1475
EaeI y'GGCC_r
Cuts at: 0 246 1413 1604 1706 2132 2174 2666 3754
Size: 246 1167 191 102 426 42 492 1088
Cuts at: 3754 4835 5178
Size: 1081 343
Fragments arranged by size:
1167 1088 1081 492 426 343 246 191
102 42
EarI CTCTTCn'nnn_
Cuts at: 0 89 250 707 1475 2465 3320 4965 5178
Size: 89 161 457 768 990 855 1645 213
Fragments arranged by size:
1645 990 855 768 457 213 161 89
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 609 5047 5082 5178
Size: 609 4438 35 96
Fragments arranged by size:
4438 609 96 35
EcoICRI GAG'CTC
Cuts at: 0 1542 5178
Size: 1542 3636
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 307 488 727 944 979 1026 1486 1724
Size: 307 181 239 217 35 47 460 238
Cuts at: 1724 1997 2225 2705 3022 3031 3352 3935 4158
Size: 273 228 480 317 9 321 583 223
Cuts at: 4158 4454 4740 4897 5178
Size: 296 286 157 281
Fragments arranged by size:
583 480 460 321 317 307 296 286
281 273 239 238 228 223 217 181
157 47 35 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 310 2984 4556 5178
Size: 310 2674 1572 622
Fragments arranged by size:
2674 1572 622 310
EcoO109I rG'GnC_Cy
Cuts at: 0 489 1054 1083 1534 2763 2977 2990 3070
Size: 489 565 29 451 1229 214 13 80
Cuts at: 3070 3405 4272 4586 5178
Size: 335 867 314 592
Fragments arranged by size:
1229 867 592 565 489 451 335 314
214 80 29 13
EcoRI G'AATT_C
Cuts at: 0 4210 5178
Size: 4210 968
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 4383 5178
Size: 4383 795
HaeII r_GCGC'y
Cuts at: 0 1763 5178
Size: 1763 3415
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 517 549 1348 1380 2723 2755 3103 3135
Size: 517 32 799 32 1343 32 348 32
Cuts at: 3135 4221 4253 5178
Size: 1086 32 925
Fragments arranged by size:
1343 1086 925 799 517 348 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 2655 2919 3458 5178
Size: 2655 264 539 1720
Fragments arranged by size:
2655 1720 539 264
HindIII A'AGCT_T
Cuts at: 0 601 5178
Size: 601 4577
HpaI GTT'AAC
Cuts at: 0 3458 5178
Size: 3458 1720
KasI G'GCGC_C
Cuts at: 0 1759 5178
Size: 1759 3419
KpnI G_GTAC'C
Cuts at: 0 70 4488 5178
Size: 70 4418 690
Fragments arranged by size:
4418 690 70
MfeI C'AATT_G
Cuts at: 0 2250 5178
Size: 2250 2928
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1027 4095 5021 5178
Size: 1027 3068 926 157
Fragments arranged by size:
3068 1027 926 157
MscI TGG'CCA
Cuts at: 0 248 1606 1708 2134 2176 2668 4837 5178
Size: 248 1358 102 426 42 492 2169 341
Fragments arranged by size:
2169 1358 492 426 341 248 102 42
MslI CAynn'nnrTG
Cuts at: 0 1169 1339 4142 5128 5178
Size: 1169 170 2803 986 50
Fragments arranged by size:
2803 1169 986 170 50
MspA1I CmG'CkG
Cuts at: 0 1215 1665 1690 2628 3048 3653 3963 4307
Size: 1215 450 25 938 420 605 310 344
Cuts at: 4307 5082 5178
Size: 775 96
Fragments arranged by size:
1215 938 775 605 450 420 344 310
96 25
NarI GG'CG_CC
Cuts at: 0 1760 5178
Size: 1760 3418
NcoI C'CATG_G
Cuts at: 0 482 494 2129 4046 4253 5178
Size: 482 12 1635 1917 207 925
Fragments arranged by size:
1917 1635 925 482 207 12
NheI G'CTAG_C
Cuts at: 0 1450 5178
Size: 1450 3728
NspI r_CATG'y
Cuts at: 0 1569 2845 4141 5178
Size: 1569 1276 1296 1037
Fragments arranged by size:
1569 1296 1276 1037
PasI CC'CwG_GG
Cuts at: 0 1086 3123 3537 5178
Size: 1086 2037 414 1641
Fragments arranged by size:
2037 1641 1086 414
PciI A'CATG_T
Cuts at: 0 1565 5178
Size: 1565 3613
PflMI CCAn_nnn'nTGG
Cuts at: 0 1368 4140 4193 4254 5018 5178
Size: 1368 2772 53 61 764 160
Fragments arranged by size:
2772 1368 764 160 61 53
PfoI T'CCnGG_A
Cuts at: 0 449 606 995 2708 2759 5159 5178
Size: 449 157 389 1713 51 2400 19
Fragments arranged by size:
2400 1713 449 389 157 51 19
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2689 2721 5178
Size: 2689 32 2457
Fragments arranged by size:
2689 2457 32
PpuMI rG'GwC_Cy
Cuts at: 0 2763 2977 2990 3070 3405 4272 4586 5178
Size: 2763 214 13 80 335 867 314 592
Fragments arranged by size:
2763 867 592 335 314 214 80 13
PspOMI G'GGCC_C
Cuts at: 0 1054 5178
Size: 1054 4124
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 2132 2164 5178
Size: 2132 32 3014
Fragments arranged by size:
3014 2132 32
PstI C_TGCA'G
Cuts at: 0 1784 3258 3924 4306 5048 5178
Size: 1784 1474 666 382 742 130
Fragments arranged by size:
1784 1474 742 666 382 130
PvuII CAG'CTG
Cuts at: 0 1215 2628 3048 5178
Size: 1215 1413 420 2130
Fragments arranged by size:
2130 1413 1215 420
SacI G_AGCT'C
Cuts at: 0 1544 5178
Size: 1544 3634
SacII CC_GC'GG
Cuts at: 0 1691 5178
Size: 1691 3487
SanDI GG'GwC_CC
Cuts at: 0 2990 3070 5178
Size: 2990 80 2108
Fragments arranged by size:
2990 2108 80
SapI GCTCTTCn'nnn_
Cuts at: 0 2465 3320 5178
Size: 2465 855 1858
Fragments arranged by size:
2465 1858 855
SbfI CC_TGCA'GG
Cuts at: 0 3258 5178
Size: 3258 1920
SexAI A'CCwGG_T
Cuts at: 0 2099 5178
Size: 2099 3079
SfcI C'TryA_G
Cuts at: 0 960 1780 2232 3254 3920 4302 5044 5178
Size: 960 820 452 1022 666 382 742 134
Fragments arranged by size:
1022 960 820 742 666 452 382 134
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 487 5178
Size: 487 4691
SfoI GGC'GCC
Cuts at: 0 1761 5178
Size: 1761 3417
SmaI CCC'GGG
Cuts at: 0 4270 4435 5178
Size: 4270 165 743
Fragments arranged by size:
4270 743 165
SmlI C'TyrA_G
Cuts at: 0 1351 1543 1960 2185 2489 2818 3031 3103
Size: 1351 192 417 225 304 329 213 72
Cuts at: 3103 5178
Size: 2075
Fragments arranged by size:
2075 1351 417 329 304 225 213 192
72
StuI AGG'CCT
Cuts at: 0 2230 2950 5178
Size: 2230 720 2228
Fragments arranged by size:
2230 2228 720
StyI C'CwwG_G
Cuts at: 0 482 494 1697 1709 2129 2210 2322 4046
Size: 482 12 1203 12 420 81 112 1724
Cuts at: 4046 4253 4716 5178
Size: 207 463 462
Fragments arranged by size:
1724 1203 482 463 462 420 207 112
81 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 313 1034 4010 5178
Size: 313 721 2976 1168
Fragments arranged by size:
2976 1168 721 313
TatI w'GTAC_w
Cuts at: 0 1568 2601 2873 5178
Size: 1568 1033 272 2305
Fragments arranged by size:
2305 1568 1033 272
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1189 1221 4104 4136 4188 4220 5178
Size: 1189 32 2883 32 52 32 958
Fragments arranged by size:
2883 1189 958 52 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 87 2965 3063 4768 5157 5178
Size: 87 2878 98 1705 389 21
Fragments arranged by size:
2878 1705 389 98 87 21
XhoI C'TCGA_G
Cuts at: 0 3031 5178
Size: 3031 2147
XmaI C'CCGG_G
Cuts at: 0 4268 4433 5178
Size: 4268 165 745
Fragments arranged by size:
4268 745 165
Enzymes that do cut:
AarI AccI Acc65I AcuI AflIII AhdI AlfI AloI
AlwNI ApaI ApoI AvaI BaeI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmrI BmtI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI BtsI DraI DraIII DrdI EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MmeI MscI MslI MspA1I NarI NcoI NheI NspI
PasI PciI PflMI PfoI PpiI PpuMI PspOMI PsrI
PstI PvuII SacI SacII SanDI SapI SbfI SexAI
SfcI SfiI SfoI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AgeI AleI ApaLI AscI
AseI AsiSI AvrII BfrBI BmgBI BplI BsiEI BsiWI
BssHII BstZ17I Bsu36I ClaI CspCI EagI EcoRV FseI
FspI FspAI MluI NaeI NdeI NgoMIV NotI NruI
NsiI PacI PmeI PmlI PshAI PsiI PspXI PvuI
RsrII SalI ScaI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI XmnI ZraI