Restriction map for
pF1KA0073
(Linear) (Six-base) MAPSORT of: KIAA0073.seq Check: 8844 from: 1 to: 1938
KIAA0073.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1270 1472 1938
Size: 557 713 202 466
Fragments arranged by size:
713 557 466 202
AfeI AGC'GCT
Cuts at: 0 145 1938
Size: 145 1793
AflIII A'CryG_T
Cuts at: 0 333 1938
Size: 333 1605
AgeI A'CCGG_T
Cuts at: 0 1157 1938
Size: 1157 781
AleI CACnn'nnGTG
Cuts at: 0 283 1938
Size: 283 1655
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 277 1259 1938
Size: 277 982 679
Fragments arranged by size:
982 679 277
AlwNI CAG_nnn'CTG
Cuts at: 0 580 1390 1648 1938
Size: 580 810 258 290
Fragments arranged by size:
810 580 290 258
ApoI r'AATT_y
Cuts at: 0 370 605 772 823 914 1015 1681 1693
Size: 370 235 167 51 91 101 666 12
Cuts at: 1693 1938
Size: 245
Fragments arranged by size:
666 370 245 235 167 101 91 51
12
AseI AT'TA_AT
Cuts at: 0 1072 1076 1938
Size: 1072 4 862
Fragments arranged by size:
1072 862 4
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1772 1805 1938
Size: 1772 33 133
Fragments arranged by size:
1772 133 33
BamHI G'GATC_C
Cuts at: 0 1765 1938
Size: 1765 173
BanI G'GyrC_C
Cuts at: 0 44 1938
Size: 44 1894
BbeI G_GCGC'C
Cuts at: 0 48 1938
Size: 48 1890
BbsI GAAGACnn'nnnn_
Cuts at: 0 710 1938
Size: 710 1228
BclI T'GATC_A
Cuts at: 0 500 1938
Size: 500 1438
BfrBI ATG'CAT
Cuts at: 0 264 1938
Size: 264 1674
Bme1580I G_kGCm'C
Cuts at: 0 1537 1938
Size: 1537 401
BmrI ACTGGGnnnn_n'
Cuts at: 0 227 691 761 801 1764 1938
Size: 227 464 70 40 963 174
Fragments arranged by size:
963 464 227 174 70 40
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1652 1938
Size: 1652 286
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1430 1938
Size: 1430 508
BsaBI GATnn'nnATC
Cuts at: 0 1391 1938
Size: 1391 547
BsaHI Gr'CG_yC
Cuts at: 0 45 1938
Size: 45 1893
BsaWI w'CCGG_w
Cuts at: 0 63 1157 1938
Size: 63 1094 781
Fragments arranged by size:
1094 781 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 741 771 1938
Size: 741 30 1167
Fragments arranged by size:
1167 741 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 145 641 749 1673 1691 1938
Size: 145 496 108 924 18 247
Fragments arranged by size:
924 496 247 145 108 18
BseYI C'CCAG_C
Cuts at: 0 146 1125 1938
Size: 146 979 813
Fragments arranged by size:
979 813 146
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1403 1938
Size: 1403 535
BsmBI CGTCTCn'nnnn_
Cuts at: 0 27 1938
Size: 27 1911
Bsp1286I G_dGCh'C
Cuts at: 0 1537 1938
Size: 1537 401
BspHI T'CATG_A
Cuts at: 0 324 762 941 1938
Size: 324 438 179 997
Fragments arranged by size:
997 438 324 179
BsrDI GCAATG_nn'
Cuts at: 0 473 1938
Size: 473 1465
BsrFI r'CCGG_y
Cuts at: 0 1157 1938
Size: 1157 781
BsrGI T'GTAC_A
Cuts at: 0 1318 1854 1938
Size: 1318 536 84
Fragments arranged by size:
1318 536 84
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1799 1938
Size: 1799 139
BstYI r'GATC_y
Cuts at: 0 545 1635 1765 1862 1938
Size: 545 1090 130 97 76
Fragments arranged by size:
1090 545 130 97 76
BtsI GCAGTG_nn'
Cuts at: 0 110 1938
Size: 110 1828
DraIII CAC_nnn'GTG
Cuts at: 0 1107 1938
Size: 1107 831
EaeI y'GGCC_r
Cuts at: 0 179 621 1020 1938
Size: 179 442 399 918
Fragments arranged by size:
918 442 399 179
EarI CTCTTCn'nnn_
Cuts at: 0 134 181 353 1938
Size: 134 47 172 1585
Fragments arranged by size:
1585 172 134 47
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 22 1938
Size: 22 1916
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1270 1472 1652 1938
Size: 557 713 202 180 286
Fragments arranged by size:
713 557 286 202 180
EcoO109I rG'GnC_Cy
Cuts at: 0 51 1456 1938
Size: 51 1405 482
Fragments arranged by size:
1405 482 51
HaeII r_GCGC'y
Cuts at: 0 48 147 1938
Size: 48 99 1791
Fragments arranged by size:
1791 99 48
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 260 292 741 773 1620 1652 1938
Size: 260 32 449 32 847 32 286
Fragments arranged by size:
847 449 286 260 32 32 32
HindIII A'AGCT_T
Cuts at: 0 1265 1938
Size: 1265 673
KasI G'GCGC_C
Cuts at: 0 44 1938
Size: 44 1894
MfeI C'AATT_G
Cuts at: 0 1204 1938
Size: 1204 734
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 132 871 1028 1663 1892 1938
Size: 132 739 157 635 229 46
Fragments arranged by size:
739 635 229 157 132 46
MscI TGG'CCA
Cuts at: 0 181 1938
Size: 181 1757
MslI CAynn'nnrTG
Cuts at: 0 269 283 1495 1938
Size: 269 14 1212 443
Fragments arranged by size:
1212 443 269 14
NarI GG'CG_CC
Cuts at: 0 45 1938
Size: 45 1893
NdeI CA'TA_TG
Cuts at: 0 1669 1938
Size: 1669 269
NsiI A_TGCA'T
Cuts at: 0 266 1938
Size: 266 1672
NspI r_CATG'y
Cuts at: 0 264 337 510 972 1938
Size: 264 73 173 462 966
Fragments arranged by size:
966 462 264 173 73
PacI TTA_AT'TAA
Cuts at: 0 1076 1938
Size: 1076 862
PasI CC'CwG_GG
Cuts at: 0 556 1938
Size: 556 1382
PciI A'CATG_T
Cuts at: 0 333 1938
Size: 333 1605
PfoI T'CCnGG_A
Cuts at: 0 528 1938
Size: 528 1410
PpuMI rG'GwC_Cy
Cuts at: 0 51 1456 1938
Size: 51 1405 482
Fragments arranged by size:
1405 482 51
PsiI TTA'TAA
Cuts at: 0 1141 1583 1938
Size: 1141 442 355
Fragments arranged by size:
1141 442 355
SanDI GG'GwC_CC
Cuts at: 0 51 1938
Size: 51 1887
SapI GCTCTTCn'nnn_
Cuts at: 0 134 1938
Size: 134 1804
SfcI C'TryA_G
Cuts at: 0 1034 1253 1938
Size: 1034 219 685
Fragments arranged by size:
1034 685 219
SfoI GGC'GCC
Cuts at: 0 46 1938
Size: 46 1892
SmlI C'TyrA_G
Cuts at: 0 1445 1938
Size: 1445 493
SspI AAT'ATT
Cuts at: 0 1191 1293 1926 1938
Size: 1191 102 633 12
Fragments arranged by size:
1191 633 102 12
StyI C'CwwG_G
Cuts at: 0 1798 1938
Size: 1798 140
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 442 1938
Size: 295 147 1496
Fragments arranged by size:
1496 295 147
TatI w'GTAC_w
Cuts at: 0 595 1318 1854 1938
Size: 595 723 536 84
Fragments arranged by size:
723 595 536 84
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1091 1123 1938
Size: 1091 32 815
Fragments arranged by size:
1091 815 32
Enzymes that do cut:
AcuI AfeI AflIII AgeI AleI AlfI AlwNI ApoI
AseI BaeI BamHI BanI BbeI BbsI BclI BfrBI
Bme1580I BmrI BpmI BpuEI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsmBI Bsp1286I BspHI BsrDI BsrFI
BsrGI BstXI BstYI BtsI DraIII EaeI EarI EciI
Eco57MI EcoO109I HaeII Hin4I HindIII KasI MfeI MmeI
MscI MslI NarI NdeI NsiI NspI PacI PasI
PciI PfoI PpuMI PsiI SanDI SapI SfcI SfoI
SmlI SspI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AhdI AloI
ApaI ApaLI AscI AsiSI AvaI AvrII BanII BbvCI
BcgI BciVI BglI BglII BlpI BmgBI BmtI BplI
Bpu10I BsaI BsaAI BsiEI BsiHKAI BsiWI BsmI BspEI
BspMI BsrBI BssHII BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraI DrdI EagI
EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HpaI KpnI MluI MspA1I NaeI NcoI NgoMIV
NheI NotI NruI PflMI PmeI PmlI PpiI PshAI
PspOMI PspXI PsrI PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI