Restriction map for
pF1KA0015
(Linear) (Six-base) MAPSORT of: KIAA0015.seq Check: 4821 from: 1 to: 1362
KIAA0015.orf
With 182 enzymes: SgfI *
October 24, 2006 15:05 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 417 1362
Size: 417 945
AatII G_ACGT'C
Cuts at: 0 1094 1362
Size: 1094 268
AflIII A'CryG_T
Cuts at: 0 518 1007 1362
Size: 518 489 355
Fragments arranged by size:
518 489 355
AleI CACnn'nnGTG
Cuts at: 0 769 1362
Size: 769 593
AlwNI CAG_nnn'CTG
Cuts at: 0 465 679 1336 1362
Size: 465 214 657 26
Fragments arranged by size:
657 465 214 26
ApaLI G'TGCA_C
Cuts at: 0 637 1362
Size: 637 725
ApoI r'AATT_y
Cuts at: 0 280 1362
Size: 280 1082
AvaI C'yCGr_G
Cuts at: 0 443 814 1034 1190 1362
Size: 443 371 220 156 172
Fragments arranged by size:
443 371 220 172 156
BanI G'GyrC_C
Cuts at: 0 429 760 1362
Size: 429 331 602
Fragments arranged by size:
602 429 331
BanII G_rGCy'C
Cuts at: 0 15 690 1051 1160 1204 1362
Size: 15 675 361 109 44 158
Fragments arranged by size:
675 361 158 109 44 15
BbsI GAAGACnn'nnnn_
Cuts at: 0 984 1224 1362
Size: 984 240 138
Fragments arranged by size:
984 240 138
BbvCI CC'TCA_GC
Cuts at: 0 47 1362
Size: 47 1315
BlpI GC'TnA_GC
Cuts at: 0 145 176 1362
Size: 145 31 1186
Fragments arranged by size:
1186 145 31
Bme1580I G_kGCm'C
Cuts at: 0 641 1362
Size: 641 721
BmgBI CAC'GTC
Cuts at: 0 804 1362
Size: 804 558
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1218 1362
Size: 1218 144
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 957 966 1279 1305 1362
Size: 29 928 9 313 26 57
Fragments arranged by size:
928 313 57 29 26 9
Bpu10I CC'TnA_GC
Cuts at: 0 47 1362
Size: 47 1315
BsaI GGTCTCn'nnnn_
Cuts at: 0 46 474 1333 1362
Size: 46 428 859 29
Fragments arranged by size:
859 428 46 29
BsaAI yAC'GTr
Cuts at: 0 519 636 1008 1362
Size: 519 117 372 354
Fragments arranged by size:
519 372 354 117
BsaHI Gr'CG_yC
Cuts at: 0 1091 1362
Size: 1091 271
BsaWI w'CCGG_w
Cuts at: 0 485 1362
Size: 485 877
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1303 1332 1333 1362
Size: 1303 29 1 29
Fragments arranged by size:
1303 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 64 169 328 331 346 1186 1317 1362
Size: 64 105 159 3 15 840 131 45
Fragments arranged by size:
840 159 131 105 64 45 15 3
BseYI C'CCAG_C
Cuts at: 0 90 1362
Size: 90 1272
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 784 1362
Size: 784 578
BsiHKAI G_wGCw'C
Cuts at: 0 146 641 1362
Size: 146 495 721
Fragments arranged by size:
721 495 146
BsmBI CGTCTCn'nnnn_
Cuts at: 0 975 1362
Size: 975 387
Bsp1286I G_dGCh'C
Cuts at: 0 15 146 641 690 1051 1160 1204 1362
Size: 15 131 495 49 361 109 44 158
Fragments arranged by size:
495 361 158 131 109 49 44 15
BspEI T'CCGG_A
Cuts at: 0 485 1362
Size: 485 877
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 417 1070 1362
Size: 417 653 292
Fragments arranged by size:
653 417 292
BsrBI CCG'CTC
Cuts at: 0 747 758 1197 1362
Size: 747 11 439 165
Fragments arranged by size:
747 439 165 11
BsrDI GCAATG_nn'
Cuts at: 0 782 1362
Size: 782 580
BsrFI r'CCGG_y
Cuts at: 0 409 512 1362
Size: 409 103 850
Fragments arranged by size:
850 409 103
BssHII G'CGCG_C
Cuts at: 0 901 1362
Size: 901 461
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1314 1362
Size: 1314 48
Bsu36I CC'TnA_GG
Cuts at: 0 680 1237 1362
Size: 680 557 125
Fragments arranged by size:
680 557 125
BtgI C'CryG_G
Cuts at: 0 121 185 1362
Size: 121 64 1177
Fragments arranged by size:
1177 121 64
BtsI GCAGTG_nn'
Cuts at: 0 466 1362
Size: 466 896
EaeI y'GGCC_r
Cuts at: 0 35 182 411 965 1362
Size: 35 147 229 554 397
Fragments arranged by size:
554 397 229 147 35
EarI CTCTTCn'nnn_
Cuts at: 0 16 305 550 1362
Size: 16 289 245 812
Fragments arranged by size:
812 289 245 16
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 29 957 966 1279 1305 1362
Size: 29 928 9 313 26 57
Fragments arranged by size:
928 313 57 29 26 9
EcoO109I rG'GnC_Cy
Cuts at: 0 111 129 210 342 959 1242 1284 1362
Size: 111 18 81 132 617 283 42 78
Fragments arranged by size:
617 283 132 111 81 78 42 18
EcoRI G'AATT_C
Cuts at: 0 280 1362
Size: 280 1082
HaeII r_GCGC'y
Cuts at: 0 1042 1362
Size: 1042 320
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1303 1332 1335 1362
Size: 1303 29 3 27
Fragments arranged by size:
1303 29 27 3
HincII GTy'rAC
Cuts at: 0 954 1362
Size: 954 408
MscI TGG'CCA
Cuts at: 0 37 184 1362
Size: 37 147 1178
Fragments arranged by size:
1178 147 37
MslI CAynn'nnrTG
Cuts at: 0 769 1222 1362
Size: 769 453 140
Fragments arranged by size:
769 453 140
MspA1I CmG'CkG
Cuts at: 0 258 456 628 1154 1362
Size: 258 198 172 526 208
Fragments arranged by size:
526 258 208 198 172
NaeI GCC'GGC
Cuts at: 0 411 1362
Size: 411 951
NcoI C'CATG_G
Cuts at: 0 121 185 1362
Size: 121 64 1177
Fragments arranged by size:
1177 121 64
NgoMIV G'CCGG_C
Cuts at: 0 409 1362
Size: 409 953
PasI CC'CwG_GG
Cuts at: 0 133 296 1362
Size: 133 163 1066
Fragments arranged by size:
1066 163 133
PflMI CCAn_nnn'nTGG
Cuts at: 0 372 1362
Size: 372 990
PfoI T'CCnGG_A
Cuts at: 0 65 1362
Size: 65 1297
PmlI CAC'GTG
Cuts at: 0 519 636 1362
Size: 519 117 726
Fragments arranged by size:
726 519 117
PpuMI rG'GwC_Cy
Cuts at: 0 111 959 1242 1284 1362
Size: 111 848 283 42 78
Fragments arranged by size:
848 283 111 78 42
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 559 591 1362
Size: 559 32 771
Fragments arranged by size:
771 559 32
PstI C_TGCA'G
Cuts at: 0 755 1362
Size: 755 607
PvuII CAG'CTG
Cuts at: 0 258 1362
Size: 258 1104
SanDI GG'GwC_CC
Cuts at: 0 959 1242 1284 1362
Size: 959 283 42 78
Fragments arranged by size:
959 283 78 42
SapI GCTCTTCn'nnn_
Cuts at: 0 16 550 1362
Size: 16 534 812
Fragments arranged by size:
812 534 16
SfcI C'TryA_G
Cuts at: 0 262 751 1362
Size: 262 489 611
Fragments arranged by size:
611 489 262
SmaI CCC'GGG
Cuts at: 0 445 1036 1192 1362
Size: 445 591 156 170
Fragments arranged by size:
591 445 170 156
SrfI GCCC'GGGC
Cuts at: 0 445 1362
Size: 445 917
StyI C'CwwG_G
Cuts at: 0 121 185 1362
Size: 121 64 1177
Fragments arranged by size:
1177 121 64
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 907 1362
Size: 907 455
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 839 871 1362
Size: 839 32 491
Fragments arranged by size:
839 491 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 192 1111 1362
Size: 192 919 251
Fragments arranged by size:
919 251 192
XmaI C'CCGG_G
Cuts at: 0 443 1034 1190 1362
Size: 443 591 156 172
Fragments arranged by size:
591 443 172 156
ZraI GAC'GTC
Cuts at: 0 1092 1362
Size: 1092 270
Enzymes that do cut:
AarI AatII AflIII AleI AlwNI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BlpI Bme1580I BmgBI BplI
BpmI Bpu10I BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BstAPI Bsu36I BtgI BtsI EaeI
EarI Eco57MI EcoO109I EcoRI HaeII Hin4I HincII MscI
MslI MspA1I NaeI NcoI NgoMIV PasI PflMI PfoI
PmlI PpuMI PsrI PstI PvuII SanDI SapI SfcI
SmaI SrfI StyI TaqII TstI XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI
AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BcgI BciVI BclI BfrBI BglI BglII
BmrI BmtI BpuEI BsaBI BsiEI BsiWI BsmI BspHI
BsrGI BssSI BstBI BstEII BstXI BstYI BstZ17I BtgZI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoNI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NdeI NheI
NotI NruI NsiI NspI PacI PciI PmeI PpiI
PshAI PsiI PspOMI PspXI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmlI SnaBI SpeI SphI SspI StuI SwaI TatI
Tth111I XbaI XhoI XmnI