HUGE

Gene/Protein Characteristic Table for KIAA1544

Link to : Rouge \| GTOP \| SWISS-PROT/TrEMBL \| GeneCards\| RefDIC
Features : DNA sequence \| Protein sequence \| Expression \| Mapping

Product ID :	ORK06103
Accession No. :	AB046764
Description :	Protein neurobeachin.
HUGO Gene Name :	neurobeachin (NBEA)
Clone Name :	fh02556 [Vector Info]
Source :	Human fetal brain

Features of the cloned DNA sequence	Description

Length: 4831 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis) for :
cloned DNA seq.
Warning for N-terminal truncation:
YES

Warning for coding interruption:
NO

Integrity of 3' end

Length of 3'UTR 1744 bp

Genome contig ID gi51511729f_34662524
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
ATCAAAAAAAGAAAAATAAAATATGCAAAGAATTC
Flanking genome sequence
(482350 - 482399)
----+----*----+----*----+----*----+----*----+----*
ACCGACTGTTTCAGATTTCATTGGACATGTCACTAGCATGCTTTCATTGT

Ensembl ContigView (Add our DAS server as a DAS source)

Chr f/r start end exon identity class

ContigView(URL based/DAS) 13 f 34704735 35144872 25 99.6 Perfect prediction

Features of the protein sequence	Description

Length: 1028 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
BAG53781	99.9	unnamed protein...	Homo sapiens
EAX08543	100.0	neurobeachin, i...	Homo sapiens
CAH72182	100.0	neurobeachin [H...	Homo sapiens
NP_056493	100.0	neurobeachin [H...	Homo sapiens
Q8NFP9	100.0	Neurobeachin; L...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other HUGE database (FASTA output, Multiple alignment)

Entry	Exp	ID%	HUGE_ID
AB011112	9.5e-75	33.8	KIAA0540
AB023210	1.7e-67	39.9	KIAA0993
AB046827	1.5e-61	38.0	KIAA1607

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method interpro_ID From To Entry Definition

HMMPfam IPR010508 48 215 PF06469 Protein of unknown function DUF1088

IPR000409 368 645 PF02138 Beige/BEACH

IPR001680 792 834 PF00400 WD40 repeat

IPR001680 976 1014 PF00400 WD40 repeat

HMMSmart IPR001680 751 788 SM00320 WD40 repeat

IPR001680 791 834 SM00320 WD40 repeat

IPR001680 851 890 SM00320 WD40 repeat

IPR001680 934 972 SM00320 WD40 repeat

IPR001680 975 1014 SM00320 WD40 repeat

ProfileScan IPR000409 356 645 PS50197 Beige/BEACH

IPR001680 940 1023 PS50294 WD40 repeat

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR010508	48	215	PF06469	Protein of unknown function DUF1088
IPR000409	368	645	PF02138	Beige/BEACH
IPR001680	792	834	PF00400	WD40 repeat
IPR001680	976	1014	PF00400	WD40 repeat
HMMSmart	IPR001680	751	788	SM00320	WD40 repeat
IPR001680	791	834	SM00320	WD40 repeat
IPR001680	851	890	SM00320	WD40 repeat
IPR001680	934	972	SM00320	WD40 repeat
IPR001680	975	1014	SM00320	WD40 repeat
ProfileScan	IPR000409	356	645	PS50197	Beige/BEACH
IPR001680	940	1023	PS50294	WD40 repeat

Prediction of transmembrane (TM) segments

Method No. N terminal transmembrane region C terminal type length

SOSUI2 1 689 LPHLTIPAVVTVTCSRLFAVNRW 711 SECONDARY 23

2 863 HEVVCVSVCAELGLVISGAKEGP 885 PRIMARY 23

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	689	LPHLTIPAVVTVTCSRLFAVNRW	711	SECONDARY	23
2	863	HEVVCVSVCAELGLVISGAKEGP	885	PRIMARY	23

Expression profile	Description

: RT-PCR-ELISA Description
: Experimental conditions

Primer_f
: CCCTGCATAATTTGAAGACAC

Primer_r
: TAGCATCAGATTATCCTCCCC

PCR conditions
: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RT-PCR-ELISA	Description

RH mapping information	Description

Chromosome No.

: 13

Experimental conditions

Panel name	: unigene
Primer_f	: -
Primer_r	: -
PCR product length	: -
PCR conditions	: -

How to obtain KIAA clone(s) Back to the HUGE Protein Database homepage

	cloned DNA seq.
Warning for N-terminal truncation:	YES
Warning for coding interruption:	NO

Length of 3'UTR	1744 bp
Genome contig ID	gi51511729f_34662524
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- ATCAAAAAAAGAAAAATAAAATATGCAAAGAATTC
Flanking genome sequence (482350 - 482399)	----+--------+--------+--------+--------+----* ACCGACTGTTTCAGATTTCATTGGACATGTCACTAGCATGCTTTCATTGT

	Chr	f/r	start	end	exon	identity	class
ContigView(URL based/DAS)	13	f	34704735	35144872	25	99.6	Perfect prediction

Primer_f	: CCCTGCATAATTTGAAGACAC
Primer_r	: TAGCATCAGATTATCCTCCCC
PCR conditions	: 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Gene/Protein Characteristic Table for KIAA1544

Features of the cloned DNA sequence

Features of the protein sequence

Expression profile

RH mapping information