Gene/Protein Characteristic Table for KIAA0082
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Product ID ORK00010
Accession No D43949
Description cap methyltransferase 1
Clone name ha02350
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4018 bp)
Predicted protein sequence (882 aa)
Flexi ORF Clone FXC00010
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0082 by Kazusa Mouse cDNA Project
Note We replaced ha01325, former representative clones for KIAA0082 with ha02350. (2003/2/07)
Features of the cloned cDNA sequence
Description

Length: 4018 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 1367 bp
Genome contig ID gi89161210f_37408995
PolyA signal sequence
(ATTAAA,-27)
+----*----+----*----+----*----+----
CTTGGATCATTAAAGATAAACATATTTTTAATGCC
Flanking genome sequence
(148273 - 148322)
----+----*----+----*----+----*----+----*----+----*
TGTGTGGCTCTGTGTTGGGGCTGCTGCTGTTTGTCCTCAGTGCTTTGTGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 6 f 37508995 37557266 24 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 882 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001117026 0 99.9 similar to CG63...
Macaca mulatta
BAF85253 0 99.9 unnamed protein...
Homo sapiens
AAI33560 0 95.2 FTSJD2 protein ...
Bos taurus
XP_001117035 0 95.0 similar to CG63...
Macaca mulatta
Q9DBC3 0 90.3 FtsJ methyltran...
Mus musculus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR000467 134 178 PF01585 D111/G-patch
IPR002877 299 498 PF01728 Ribosomal RNA methyltransferase RrmJ/FtsJ
IPR001202 801 831 PF00397 WW/Rsp5/WWP
HMMSmart IPR000467 132 178 SM00443 D111/G-patch
IPR001202 800 833 SM00456 WW/Rsp5/WWP
ProfileScan IPR000467 134 180 PS50174 D111/G-patch
ScanRegExp IPR001202 805 831 PS01159 WW/Rsp5/WWP
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name Stanford G3
Primer_f TGTGGGCTCTGCTGTTCTCTC
Primer_r TGAGTGGGGGAAGGAGACAAG
PCR product length 247 bp
PCR conditions 95 °C15 sec66 °C120 sec30 cycles
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