KIAA0085

Product ID	ORK00402
Accession No	D42042
Description	lysosomal protein transmembrane 5
Clone name	ha01520
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2169 bp) Predicted protein sequence (269 aa)
HaloTag ORF Clone	FHC00402
Flexi ORF Clone	FXC00402
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0085 by Kazusa Mouse cDNA Project

Length: 2169 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	1359 bp
Genome contig ID	gi89161185r_30877903
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- AGGGAGGAAGTTTCAATAAAGCAGCAACAAGCTTC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* TGTCTGGCTGATGTGTTTCTGGAAATACAGTGTAGGGAGGGTGGAGACCA

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	30977903	31003200	8	99.2	Perfect prediction

Length: 269 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q13571	1.1e-106	100.0	Lysosomal-assoc...	Homo sapiens
AAV38801	1.1e-106	100.0	Lysosomal-assoc...	synthetic construct
AAI06897	2.1e-106	99.6	Lysosomal multi...	Homo sapiens
AAA74018	2.8e-106	99.6	E3 protein.	Homo sapiens
Q5REZ0	2.9e-105	98.1	Lysosomal-assoc...	Pongo abelii

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004687	36	268	PF03821	Golgi 4-transmembrane spanning transporter
HMMTigr	IPR004687	11	269	TIGR00799	Golgi 4-transmembrane spanning transporter

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	24	VRIATTALAIYHVIMSVLLFIEH	46	PRIMARY	23
	2	71	ISSFLLITMLFIISLSLLIGVVK	93	PRIMARY	23
	3	99	LLPFLSLQIMDYLLCLLTLLGSY	121	PRIMARY	23
	4	141	FPLMTLQLLDFCLSILTLCSSYM	163	SECONDARY	23
	5	191	FIKMMIIFSIAFITVLIFKVYMF	213	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	TGCTCACCTATTCAGTTGCTC
Primer_r	CTTTGACTGAACTAACCTGTG
PCR product length	243 bp
PCR conditions	95 °C15 sec62 °C120 sec30 cycles