##################################
# NO CUT ENZYMES ( 120 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AgeI Alw44I Aor51HI ApaLI
AscI Asp718I AspI AviII AvrII
BamHI BanI BbeI BbrPI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstYI BstZ17I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HaeII
HhaI HinP1I HpaI KasI KpnI
KspI MflI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SalI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
XbaI XhoI XhoII XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 628 GTCTAC GT^[AC][GT]AC
AcyI 162 GGCGTC G[GA]^CG[CT]C
AflIII 632 ACATGT A^C[GA][CT]GT
AhdI 1537 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 197 GGATC GGATC(4/5)
AocI 192 CCTCAGG CC^T[ACGT]AGG
AseI 1267 ATTAAT AT^TAAT
AsnI 1267 ATTAAT AT^TAAT
Asp700I 1790 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1537 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 762 GAAGAC GAAGAC(2/6)
BfaI 407 CTAG C^TAG
BpuAI 762 GAAGAC GAAGAC(2/6)
BsaHI 162 GGCGTC G[GA]^CG[CT]C
BspHI 110 TCATGA T^CATGA
BspLU11I 632 ACATGT A^CATGT
BstXI 83 CCACCGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 192 CCTCAGG CC^T[ACGT]AGG
Eam1105I 1537 GACAAACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 192 CCTCAGG CC^T[ACGT]AGG
EcoNI 958 CCTCCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 162 GGCGTC G[GA]^CG[CT]C
HindIII 2163 AAGCTT A^AGCTT
MaeI 407 CTAG C^TAG
MaeII 1416 ACGT A^CGT
MfeI 1610 CAATTG C^AATTG
MunI 1610 CAATTG C^AATTG
NheI 406 GCTAGC G^CTAGC
NspI 632 ACATGT [GA]CATG^[CT]
PshBI 1267 ATTAAT AT^TAAT
RcaI 110 TCATGA T^CATGA
SacI 2090 GAGCTC GAGCT^C
SapI 237 GCTCTTC GCTCTTC(1/4)
SbfI 961 CCTGCAGG CCTGCA^GG
SfiI 881 GGCCCCTGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 382 CTCAAG C^T[CT][GA]AG
Sse8387I 961 CCTGCAGG CCTGCA^GG
XmnI 1790 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AfaI 291 GTAC GT^AC
2066 GTAC
AlwNI 439 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
965 CAGGGCCTG
ApaI 777 GGGCCC GGGCC^C
899 GGGCCC
AspHI 133 GAGCAC G[AT]GC[AT]^C
2090 GAGCTC
AvaI 1017 CTCGGG C^[CT]CG[GA]G
1802 CCCGAG
BcnI 282 CCGGG CC^[GC]GG
395 CCCGG
BglI 882 GCCCCTGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2031 GCCACGGAGGC
BsaBI 579 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
582 GATGATCATC
BsaI 1021 GGTCTC GGTCTC(1/5)
2044 GGTCTC
BsiHKAI 133 GAGCAC G[AT]GC[AT]^C
2090 GAGCTC
BsoBI 1017 CTCGGG C^[CT]CG[GA]G
1802 CCCGAG
BspMI 52 ACCTGC ACCTGC(4/8)
293 ACCTGC
DraI 1405 TTTAAA TTT^AAA
1526 TTTAAA
EcoT14I 20 CCATGG C^C[AT][AT]GG
153 CCATGG
HapII 282 CCGG C^CGG
396 CCGG
HincII 992 GTCAAC GT[CT]^[GA]AC
1534 GTTGAC
HindII 992 GTCAAC GT[CT]^[GA]AC
1534 GTTGAC
HpaII 282 CCGG C^CGG
396 CCGG
MamI 579 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
582 GATGATCATC
MspA1I 439 CAGCTG C[AC]G^C[GT]G
1944 CAGCTG
MspI 282 CCGG C^CGG
396 CCGG
NciI 282 CCGGG CC^[GC]GG
395 CCCGG
NcoI 20 CCATGG C^CATGG
153 CCATGG
PflMI 714 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCATCAACTGG
PpuMI 1481 AGGACCT [GA]G^G[AT]CC[CT]
1999 GGGTCCT
PvuII 439 CAGCTG CAG^CTG
1944 CAGCTG
RsaI 291 GTAC GT^AC
2066 GTAC
StyI 20 CCATGG C^C[AT][AT]GG
153 CCATGG
Tsp45I 807 GTGAC ^GT[GC]AC
1010 GTCAC
Van91I 714 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1450 CCATCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BalI BanII BbvI BclI BmyI
BpmI BsaJI BseRI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI FbaI Fnu4HI
FokI HaeIII HgaI HinfI HphI
ItaI Ksp632I MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XcmI