!!NA_SEQUENCE 1.0
LOCUS       PSPORT1      4109 bp    DNA             SYN       06-DEC-1999
DEFINITION  Cloning vector pSport1, complete cds.
ACCESSION   U12390
VERSION     U12390.1  GI:531828
KEYWORDS    .
SOURCE      Cloning vector pSport1.
  ORGANISM  Cloning vector pSport1
            artificial sequence; vectors.
REFERENCE   1  (sites)
  AUTHORS   D'Alessio,J.M., Gruber,C.E., Cain,C. and Noon,M.C.
  TITLE     Construction of Directional cDNA Libraries Using the Superscript
            Plasmid System
  JOURNAL   Focus 12, 47-50 (1990)
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Horton,M.E.
  TITLE     Direct Submission
  JOURNAL   Submitted (18-JUL-1994) Horton M. E., Life Technologies, Inc.,
            Technical Services, 8400 Helgerman Court, Gaithersburg, MD
            20884-9980, USA
FEATURES             Location/Qualifiers
     source          1. .4109
                     /organism="Cloning vector pSport1"
                     /db_xref="taxon:36586"
     CDS             complement(<1. .341)
                     /codon_start=1
                     /transl_table=11
                     /evidence=experimental
                     /product="beta-galactosidase alpha peptide"
                     /protein_id="AAA73456.2"
                     /db_xref="GI:6525246"
                     /translation="MTMITPSSNTTHYRESWYACRYRSGIPGSTSSLVGGRSRGSKLT
                     YACMRRHSSSIVSPKFNSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDR
                     PSQQLRSLNGE"
     primer_bind     complement(125. .147)
                     /standard_name="M13/pUC 23 base forward sequencing primer"
     primer_bind     complement(150. .161)
                     /standard_name="M13/pUC forward sequencing primer"
                     /evidence=experimental
     promoter        164. .180
                     /note="SP6 promoter"
                     /evidence=experimental
     misc_feature    191. .285
                     /function="multiple cloning site"
                     /evidence=experimental
     promoter        complement(298. .318)
                     /note="T7 promoter"
                     /evidence=experimental
     CDS             complement(464. .1546)
                     /codon_start=1
                     /transl_except=(pos:complement(1544. .1546),aa:Met)
                     /transl_table=11
                     /evidence=experimental
                     /product="lac repressor"
                     /protein_id="AAA73457.1"
                     /db_xref="GI:531830"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     rep_origin      complement(1896. .1902)
                     /standard_name="ORI E. coli pMB1 (ColE1 and pBR322)"
                     /evidence=experimental
     CDS             complement(2661. .3521)
                     /function="ampicillin resistance"
                     /codon_start=1
                     /transl_table=11
                     /evidence=experimental
                     /product="beta-lactamase"
                     /protein_id="AAA73458.1"
                     /db_xref="GI:531831"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     rep_origin      3667. .4109
                     /note="F1 intergenic region"
                     /evidence=experimental
BASE COUNT     1002 a   1064 c   1062 g    981 t
ORIGIN

   U12390  Length: 4109  May 26, 2000 16:28  Type: N  Check: 1267  ..

       1  CATTCGCCAT TCAGGCTGCG CAACTGTTGG GAAGGGCGAT CGGTGCGGGC 

      51  CTCTTCGCTA TTACGCCAGC TGGCGAAAGG GGGATGTGCT GCAAGGCGAT 

     101  TAAGTTGGGT AACGCCAGGG TTTTCCCAGT CACGACGTTG TAAAACGACG 

     151  GCCAGTGAAT TGAATTTAGG TGACACTATA GAAGAGCTAT GACGTCGCAT 

     201  GCACGCGTAC GTAAGCTTGG ATCCTCTAGA GCGGCCGCCG ACTAGTGAGC 

     251  TCGTCGACCC GGGAATTCCG GACCGGTACC TGCAGGCGTA CCAGCTTTCC 

     301  CTATAGTGAG TCGTATTAGA GCTTGGCGTA ATCATGGTCA TAGCTGTTTC 

     351  CTGTGTGAAA TTGTTATCCG CTCACAATTC CACACAACAT ACGAGCCGGA 

     401  AGCATAAAGT GTAAAGCCTG GGGTGCCTAA TGAGTGAGCT AACTCACATT 

     451  AATTGCGTTG CGCTCACTGC CCGCTTTCCA GTCGGGAAAC CTGTCGTGCC 

     501  AGCTGCATTA ATGAATCGGC CAACGCGCGG GGAGAGGCGG TTTGCGTATT 

     551  GGGCGCCAGG GTGGTTTTTC TTTTCACCAG TGAGACGGGC AACAGCTGAT 

     601  TGCCCTTCAC CGCCTGGCCC TGAGAGAGTT GCAGCAAGCG GTCCACGCTG 

     651  GTTTGCCCCA GCAGGCGAAA ATCCTGTTTG ATGGTGGTTG ACGGCGGGAT 

     701  ATAACATGAG CTGTCTTCGG TATCGTCGTA TCCCACTACC GAGATATCCG 

     751  CACCAACGCG CAGCCCGGAC TCGGTAATGG CGCGCATTGC GCCCAGCGCC 

     801  ATCTGATCGT TGGCAACCAG CATCGCAGTG GGAACGATGC CCTCATTCAG 

     851  CATTTGCATG GTTTGTTGAA AACCGGACAT GGCACTCCAG TCGCCTTCCC 

     901  GTTCCGCTAT CGGCTGAATT TGATTGCGAG TGAGATATTT ATGCCAGCCA 

     951  GCCAGACGCA GACGCGCCGA GACAGAACTT AATGGGCCCG CTAACAGCGC 

    1001  GATTTGCTGG TGACCCAATG CGACCAGATG CTCCACGCCC AGTCGCGTAC 

    1051  CGTCTTCATG GGAGAAAATA ATACTGTTGA TGGGTGTCTG GTCAGAGACA 

    1101  TCAAGAAATA ACGCCGGAAC ATTAGTGCAG GCAGCTTCCA CAGCAATGGC 

    1151  ATCCTGGTCA TCCAGCGGAT AGTTAATGAT CAGCCCACTG ACCCGTTGCG 

    1201  CGAGAAGATT GTGCACCGCC GCTTTACAGG CTTCGACGCC GCTTCGTTCT 

    1251  ACCATCGACA CCACCACGCT GGCACCCAGT TGATCGGCGC GAGATTTAAT 

    1301  CGCCGCGACA ATTTGCGACG GCGCGTGCAG GGCCAGACTG GAGGTGGCAA 

    1351  CGCCAATCAG CAACGACTGT TTGCCCGCCA GTTGTTGTGC CACGCGGTTG 

    1401  GGAATGTAAT TCAGCTCCGC CATCGCCGCT TCCACTTTTT CCCGCGTTTT 

    1451  CGCAGAAACG TGGCTGGCCT GGTTCACCAC GCGGGAAACG GTCTGATAAG 

    1501  AGACACCGGC ATACTCTGCG ACATCGTATA ACGTTACTGG TTTCACATTC 

    1551  ACCACCCTGA ATTGACTCTC TTCCGGGCGC TATCATGCCA TACCGCGAAA 

    1601  GGTTTTGCGC CATTCGATGG TGTCAACGTA AATGCCGCTT CGCCTTCGCG 

    1651  CGCGAATTGC AAGCTCTGCA TTAATGAATC GGCCAACGCG CGGGGAGAGG 

    1701  CGGTTTGCGT ATTGGGCGCT CTTCCGCTTC CTCGCTCACT GACTCGCTGC 

    1751  GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 

    1801  ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 

    1851  AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 

    1901  TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA 

    1951  AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 

    2001  CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 

    2051  GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCAATGC 

    2101  TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 

    2151  CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 

    2201  ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA 

    2251  GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 

    2301  AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGGACAGTAT 

    2351  TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 

    2401  AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 

    2451  TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 

    2501  TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA 

    2551  GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 

    2601  AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAAACTT 

    2651  GGTCTGACAG TTACCAATGC TTAATCAGTG AGGCACCTAT CTCAGCGATC 

    2701  TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG TGTAGATAAC 

    2751  TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 

    2801  GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC 

    2851  GGAAGGGCCG AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA 

    2901  GTCTATTAAT TGTTGCCGGG AAGCTAGAGT AAGTAGTTCG CCAGTTAATA 

    2951  GTTTGCGCAA CGTTGTTGCC ATTGCTACAG GCATCGTGGT GTCACGCTCG 

    3001  TCGTTTGGTA TGGCTTCATT CAGCTCCGGT TCCCAACGAT CAAGGCGAGT 

    3051  TACATGATCC CCCATGTTGT GCAAAAAAGC GGTTAGCTCC TTCGGTCCTC 

    3101  CGATCGTTGT CAGAAGTAAG TTGGCCGCAG TGTTATCACT CATGGTTATG 

    3151  GCAGCACTGC ATAATTCTCT TACTGTCATG CCATCCGTAA GATGCTTTTC 

    3201  TGTGACTGGT GAGTACTCAA CCAAGTCATT CTGAGAATAG TGTATGCGGC 

    3251  GACCGAGTTG CTCTTGCCCG GCGTCAATAC GGGATAATAC CGCGCCACAT 

    3301  AGCAGAACTT TAAAAGTGCT CATCATTGGA AAACGTTCTT CGGGGCGAAA 

    3351  ACTCTCAAGG ATCTTACCGC TGTTGAGATC CAGTTCGATG TAACCCACTC 

    3401  GTGCACCCAA CTGATCTTCA GCATCTTTTA CTTTCACCAG CGTTTCTGGG 

    3451  TGAGCAAAAA CAGGAAGGCA AAATGCCGCA AAAAAGGGAA TAAGGGCGAC 

    3501  ACGGAAATGT TGAATACTCA TACTCTTCCT TTTTCAATAT TATTGAAGCA 

    3551  TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 

    3601  AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC 

    3651  TGAAATTGTA AACGTTAATA TTTTGTTAAA ATTCGCGTTA AATTTTTGTT 

    3701  AAATCAGCTC ATTTTTTAAC CAATAGGCCG AAATCGGCAA AATCCCTTAT 

    3751  AAATCAAAAG AATAGACCGA GATAGGGTTG AGTGTTGTTC CAGTTTGGAA 

    3801  CAAGAGTCCA CTATTAAAGA ACGTGGACTC CAACGTCAAA GGGCGAAAAA 

    3851  CCGTCTATCA GGGCGATGGC CCACTACGTG AACCATCACC CTAATCAAGT 

    3901  TTTTTGGGGT CGAGGTGCCG TAAAGCACTA AATCGGAACC CTAAAGGGAG 

    3951  CCCCCGATTT AGAGCTTGAC GGGGAAAGCC GGCGAACGTG GCGAGAAAGG 

    4001  AAGGGAAGAA AGCGAAAGGA GCGGGCGCTA GGGCGCTGGC AAGTGTAGCG 

    4051  GTCACGCTGC GCGTAACCAC CACACCCGCC GCGCTTAATG CGCCGCTACA 

    4101  GGGCGCGTC