Gene/Protein Characteristic Table for KIAA0083
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Product ID ORK00400
Accession No D42046
Description DNA replication helicase/nuclease 2, transcript variant 1
Clone name ha03631
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (4207 bp)
Predicted protein sequence (1077 aa)
Flexi ORF Clone FXC00400
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0083 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 4207 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 973 bp
Genome contig ID gi89161187r_69743883
PolyA signal sequence
(AATAAA,-22)
+----*----+----*----+----*----+----
AAGTTTTTTATTTAATAAATTTTTTCTACTAATGG
Flanking genome sequence
(99945 - 99896)
----+----*----+----*----+----*----+----*----+----*
ATCTTAACTGTGTCAATTATTATTAATACTGAATAATAATTAAACTCAGG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 10 r 69843828 69901678 21 99.8 Perfect prediction
Features of the protein sequence
Description

Length: 1077 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
NP_001073918 0 100.0 DNA replication...
Homo sapiens
XP_001163213 0 99.4 DNA2 DNA replic...
Pan troglodytes
P51530 0 100.0 DNA2-like helic...
Homo sapiens
EAW54296 0 98.8 hCG32858 [Homo ...
Homo sapiens
CAI17238 0 100.0 DNA2 DNA replic...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
D86988 4.5e-12 29.9 KIAA0221
AB037825 6.1e-09 27.9 KIAA1404
AB014525 1.8e-07 30.4 KIAA0625
D29677 1.3e-06 24.0 KIAA0054
AB051556 4.4e-06 27.2 KIAA1769
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR014808 78 300 PF08696 DNA replication factor Dna2
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 10
Experimental conditions
Panel name Genebridge 4
Primer_f CTATGTTTGGGGGTATCACTC
Primer_r AACCCTCAAGAGAAATGGAAG
PCR product length 180 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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