KIAA0040

Product ID	ORK05581
Accession No	D25539
Description	KIAA0040
Clone name	ha01160
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4460 bp) Predicted protein sequence (164 aa)
HaloTag ORF Clone	FHC05581
Flexi ORF Clone	FXC05581
Source	Myeloblast cell line (KG-1)

Length: 4460 bp

Physical map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	3183 bp
Genome contig ID	gi89161185r_173292867
PolyA signal sequence (AATAAA,-25)	+--------+--------+----*----+---- ATTGCAATGAAATAAAATTAAAGGTATACTAGCTC
Flanking genome sequence (99879 - 99830)	----+--------+--------+--------+--------+----* AGCTATGTCTGCCATATTTCTTTTTTCTTTTTTTTTTTTTTTTTTTTTTG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	1	r	173392746	173428456	4	99.1	Perfect prediction

Length: 164 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

No significant homologues

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	44	QFLDTVLAAMCPIAMLLTADPGM	66	SECONDARY	23
SOSUI2	2	107	SRYVGKYILLIKWSLAMYFVQGS	129	PRIMARY	23

Chromosome No. 1

Experimental conditions