##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AcyI AflII AgeI Aor51HI
ApaI AscI AvaI AviII BbeI
BcgI BfrI BglII BsaHI BsiEI
BsiWI BsoBI BspDI BsrBI BsrFI
BssHII BssSI CfoI Cfr10I ClaI
DraIII EagI EclXI Eco47III Eco52I
EcoRI FseI FspI HaeII HgaI
HhaI Hin1I HinP1I HincII HindII
HpaI KasI MfeI MluI MunI
NaeI NarI NdeI NgoMIV NheI
NotI NruI PacI PaeR7I PflMI
PinAI PmeI PvuI SalI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Van91I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
Acc65I 4393 GGTACC G^GTACC
AccI 4449 GTATAC GT^[AC][GT]AC
AccII 46 CGCG CG^CG
AccIII 3674 TCCGGA T^CCGGA
AclI 3310 AACGTT AA^CGTT
AseI 2410 ATTAAT AT^TAAT
AsnI 2410 ATTAAT AT^TAAT
Asp718I 4393 GGTACC G^GTACC
AspI 1502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3639 CCTAGG C^CTAGG
BamHI 2999 GGATCC G^GATCC
BbrPI 904 CACGTG CAC^GTG
BbvCI 2182 GCTGAGG CCTCAGC(-5/-2)
BciVI 920 GGATAC GTATCC(6/5)
BlnI 3639 CCTAGG C^CTAGG
BlpI 766 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 766 GCTAAGC GC^T[ACGT]AGC
BsaBI 143 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3674 TCCGGA [AT]^CCGG[AT]
BseAI 3674 TCCGGA T^CCGGA
BsmBI 2958 GAGACG CGTCTC(1/5)
BsmI 154 GAATGC GAATGC(1/-1)
Bsp1407I 4366 TGTACA T^GTACA
BspEI 3674 TCCGGA T^CCGGA
BspHI 1498 TCATGA T^CATGA
BsrGI 4366 TGTACA T^GTACA
Bst1107I 4449 GTATAC GTA^TAC
BstAPI 2309 GCAAGTTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 3858 TTCGAA TT^CGAA
BstUI 46 CGCG CG^CG
BstZ17I 4449 GTATAC GTA^TAC
CelII 766 GCTAAGC GC^T[ACGT]AGC
CpoI 48 CGGACCG CG^G[AT]CCG
DrdI 4007 GACACAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 3360 GATATC GAT^ATC
EcoT22I 1294 ATGCAT ATGCA^T
KpnI 4393 GGTACC GGTAC^C
KspI 45 CCGCGG CCGC^GG
MamI 143 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3674 TCCGGA T^CCGGA
MvnI 46 CGCG CG^CG
NsiI 1294 ATGCAT ATGCA^T
NspV 3858 TTCGAA TT^CGAA
PflFI 1502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 904 CACGTG CAC^GTG
PmlI 904 CACGTG CAC^GTG
PshAI 3059 GACAGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2410 ATTAAT AT^TAAT
Psp1406I 3310 AACGTT AA^CGTT
RcaI 1498 TCATGA T^CATGA
RsrII 48 CGGACCG CG^G[AT]CCG
SacII 45 CCGCGG CCGC^GG
SapI 3207 GAAGAGC GCTCTTC(1/4)
SbfI 29 CCTGCAGG CCTGCA^GG
ScaI 1340 AGTACT AGT^ACT
SfuI 3858 TTCGAA TT^CGAA
SmlI 2142 CTTGAG C^T[CT][GA]AG
Sse8387I 29 CCTGCAGG CCTGCA^GG
SspBI 4366 TGTACA T^GTACA
SspI 2361 AATATT AAT^ATT
TaqI 3859 TCGA T^CGA
Tth111I 1502 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3859 TCGA T^CGA
XbaI 2320 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AhdI 869 GACTAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACGTAGTGTC
Alw44I 1062 GTGCAC G^TGCAC
1846 GTGCAC
ApaLI 1062 GTGCAC G^TGCAC
1846 GTGCAC
Asp700I 36 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2375 GAAGCTATTC
AspEI 869 GACTAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACGTAGTGTC
BalI 283 TGGCCA TGG^CCA
2670 TGGCCA
BanI 4137 GGTGCC G^G[CT][GA]CC
4393 GGTACC
BbsI 664 GAAGAC GAAGAC(2/6)
2847 GAAGAC
BcnI 2996 CCGGG CC^[GC]GG
3003 CCGGG
BglI 599 GCCCACCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4323 GCCACTAGGGC
BpuAI 664 GAAGAC GAAGAC(2/6)
2847 GAAGAC
BsaI 711 GAGACC GGTCTC(1/5)
4118 GAGACC
BstEII 2569 GGTGACC G^GT[ACGT]ACC
4031 GGTCACC
BstPI 2569 GGTGACC G^GT[ACGT]ACC
4031 GGTCACC
BstXI 518 CCATCTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2836 CCAGGGATGTGG
Eam1105I 869 GACTAAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2960 GACGTAGTGTC
EcoNI 111 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1731 CCTGAGCTAGG
EcoO65I 2569 GGTGACC G^GT[ACGT]ACC
4031 GGTCACC
HindIII 691 AAGCTT A^AGCTT
2019 AAGCTT
MluNI 283 TGGCCA TGG^CCA
2670 TGGCCA
MscI 283 TGGCCA TGG^CCA
2670 TGGCCA
NciI 2996 CCGGG CC^[GC]GG
3003 CCGGG
PpuMI 389 AGGACCT [GA]G^G[AT]CC[CT]
3521 GGGACCT
PstI 30 CTGCAG CTGCA^G
964 CTGCAG
PvuII 100 CAGCTG CAG^CTG
2953 CAGCTG
SacI 383 GAGCTC GAGCT^C
1617 GAGCTC
SwaI 3884 ATTTAAAT ATTT^AAAT
4416 ATTTAAAT
XmnI 36 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2375 GAAGCTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BanII BbvI BclI BfaI
BmyI BpmI BsaAI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspLU11I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraI DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NcoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SphI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII