The ORFeome clones are subjected to the quality checks described below
before being presented on our web site. The number of ORFeome clones
we can provide will be gradually increased under our quality assurance standards.
Confirmation of the sequences of 3' end of ORF.
3' end of each ORF is confirmed by single-pass sequencing using
TempliPhi-amplified DNA (illustraTM TempliPhiTM DNA Sequencing
Template Amplification Kit, GE Healthcare) and GatewaySeqL-B primer:
GTAACATCAGAGATTTTGAGACAC.
The sequences of the 200 bp of the 3' ends of ORFs are compared with
those obtained from the corresponding Genbank entries after
eliminating
the flanking sequences. We opend the ORFeome clones which are
considered as the correct ones by the sequence comparison.