KIAA0001 sequence was obtained by assembly of
sequences derived from two clones. ha00008 retains
sequence from nt. positions 757 to 2416 of KIAA0001.
The cDNA fragment was cloned by use of ZAP-cDNA
synthesis kit and inserted at the EcoRI-XhoI site of
the pNTT104 vector (GenBank Accession No. AB032066, AmpR).
The 5' end of the cDNA was repaired and ligated with
the EcoRI adapter (5'AATTCGGCACGAG3') and inserted
at the EcoRI site of the vector. The 3' end of the cDNA
was inserted at the XhoI site of the vector. The other
clone a240 harbors sequence from nt. positions 1 to
795 of KIAA0001 which was amplified by RACE and
cloned into M13mp18 vector (GenBank Accession No.