!!NA_SEQUENCE 1.0
LOCUS       AB032066     3297 bp    DNA   circular  SYN       11-SEP-1999
DEFINITION  Cloning vector pNTT104 DNA, complete sequence.
ACCESSION   AB032066
VERSION     AB032066.1  GI:5869538
KEYWORDS    .
SOURCE      Cloning vector pNTT104 (lab_host:Escherichia coli) DNA.
  ORGANISM  Cloning vector pNTT104
            artificial sequence; vectors.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Nomura,N. and Nagase,T.
  TITLE     The nucleotide sequence of the pNTT104 vector
  JOURNAL   Published Only in DataBase (1999) In press
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Nomura,N. and Nagase,T.
  TITLE     Direct Submission
  JOURNAL   Submitted (31-AUG-1999) to the DDBJ/EMBL/GenBank databases. Nobuo
            Nomura, Kazusa DNA Research Institute, Laboratory of Gene Structure
            1; 1532-3 Yana, Kisarazu, Chiba 292-0812, Japan
            (E-mail:nomura@kazusa.or.jp, URL:http://www.kazusa.or.jp,
            Tel:81-438-52-3932, Fax:81-438-52-3931)
COMMENT     pNTT104
            This sequence is derived from the pUC118 sequence mainly compiled
            by changing the polylinker sequence (sequence at the position
            between 231 and 291 of the pUC118) to the 194-bp sequence (sequence
            at the position between 231 and 426 of the pNTT104) containing
            multiple restriction enzyme recognition sites. In addition to that,
            both the ScaI and NarI sites of the pUC118 were eliminated by
            nucleotide exchanges at the positions 586 (C to T) and 1802 (G to
            A) of the pNTT104 sequence, respectively.
FEATURES             Location/Qualifiers
     source          1. .3297
                     /organism="Cloning vector pNTT104"
                     /db_xref="taxon:103691"
                     /lab_host="Escherichia coli"
     misc_feature    205. .462
                     /note="complementary base sequence was reported as
                     Acc#:D16587; polylinker"
BASE COUNT      789 a    851 c    818 g    839 t
ORIGIN

 AB032066  Length: 3297  June 19, 2001 19:42  Type: N  Check: 4932  ..

       1  AGCGCCCAAT ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA 

      51  TGCAGCTGGC ACGACAGGTT TCCCGACTGG AAAGCGGGCA GTGAGCGCAA 

     101  CGCAATTAAT GTGAGTTAGC TCACTCATTA GGCACCCCAG GCTTTACACT 

     151  TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG ATAACAATTT 

     201  CACACAGGAA ACAGCTATGA CCATGATTAC GGCATGCCTG CAGGCGCCGA 

     251  CGTCATGCAT GGGCCCTCTA GATCTAGTAC TAGTGATATC GATCGGTCCG 

     301  TACGTTTTTC AGCACGCGTT AAAAAAACCA TCCCCCTGGA AGCTTAAGGC 

     351  CTCGAGTTCG AATTCGCGGC CGCACGTGTC CATGGATCCC GGGTCGACGG 

     401  CCGCGGTGGC CGGTACCGAG CTCATACTGG CCGTCGTTTT ACAACGTCGT 

     451  GACTGGGAAA ACCCTGGCGT TACCCAACTT AATCGCCTTG CAGCACATCC 

     501  CCCTTTCGCC AGCTGGCGTA ATAGCGAAGA GGCCCGCACC GATCGCCCTT 

     551  CCCAACAGTT GCGCAGCCTG AATGGCGAAT GGCGCTTGAT GCGGTATTTT 

     601  CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATACGTC AAAGCAACCA 

     651  TAGTACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC 

     701  GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG 

     751  CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 

     801  CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT 

     851  CGACCCCAAA AAACTTGATT TGGGTGATGG TTCACGTAGT GGGCCATCGC 

     901  CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 

     951  AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGGCTA 

    1001  TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA 

    1051  ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTTAACAA AATATTAACG 

    1101  TTTACAATTT TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA 

    1151  GTTAAGCCAG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG 

    1201  CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG 

    1251  AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGACG 

    1301  AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA 

    1351  TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC 

    1401  CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG 

    1451  ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA 

    1501  GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC 

    1551  CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA 

    1601  AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG 

    1651  GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC 

    1701  ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTA TTGACGCCGG 

    1751  GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG 

    1801  AATACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA 

    1851  GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT 

    1901  ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA 

    1951  ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT 

    2001  GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG TAGCAATGGC 

    2051  AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC 

    2101  GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT 

    2151  CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC 

    2201  CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA 

    2251  AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG 

    2301  GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA 

    2351  TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA 

    2401  AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT 

    2451  CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA 

    2501  CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG 

    2551  TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT 

    2601  TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG 

    2651  CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC 

    2701  ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC 

    2751  CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT 

    2801  GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG 

    2851  GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG 

    2901  AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG 

    2951  AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA 

    3001  CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG 

    3051  TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG 

    3101  GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG 

    3151  CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT 

    3201  TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG 

    3251  CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAG