FLJ00220

Product ID	ORK04562
Accession No	AK074147
Clone name	sj05828
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACGCGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4240 bp) Predicted protein sequence (503 aa)
Source	Human spleen
Rouge ID	mFLJ00220 by Kazusa Mouse cDNA Project

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Length: 503 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
BAB84973	1.7e-193	100.0	FLJ00220 protei...	Homo sapiens
BAH11513	3.1e-162	99.1	unnamed protein...	Homo sapiens
AAQ89285	3.3e-162	99.1	chordin [Homo s...	Homo sapiens
Q9H2X0	3.3e-162	99.1	Chordin; Flags:...	Homo sapiens
AAG35767	5.5e-162	98.8	chordin [Homo s...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against other entries from Kazusa human cDNA project (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR010895	15	112	PF07452	CHRD
	IPR010895	117	234	PF07452	CHRD
	IPR010895	245	360	PF07452	CHRD
	IPR001007	418	475	PF00093	von Willebrand factor
HMMSmart	IPR001007	418	475	SM00214	von Willebrand factor
ProfileScan	IPR001007	416	476	PS50184	von Willebrand factor
ScanRegExp	IPR001007	439	475	PS01208	von Willebrand factor

Primer_f
Primer_r
PCR conditions	test