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Gene/Protein Characteristic Table for KIAA0313

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Product ID	ORK00500
Accession No	AB002311
Description	Rap guanine nucleotide exchange factor (GEF) 2
Clone name	hg00186
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6568 bp) Predicted protein sequence (1508 aa)
HaloTag ORF Clone	FHC00500
Flexi ORF Clone	FXC00500
Source	Human adult brain
Rouge ID	mKIAA0313 by Kazusa Mouse cDNA Project

Length: 6568 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1508 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR000595	163	249	PF00027	Cyclic nucleotide-binding
	IPR000651	279	366	PF00618	Guanine nucleotide exchange factor for Ras-like GTPases
	IPR001478	419	473	PF00595	PDZ/DHR/GLGF
	IPR000159	615	701	PF00788	Ras-association
	IPR001895	723	908	PF00617	Guanine-nucleotide dissociation stimulator CDC25
HMMSmart	IPR000595	144	262	SM00100	Cyclic nucleotide-binding
	IPR000651	276	389	SM00229	Guanine nucleotide exchange factor for Ras-like GTPases
	IPR001478	404	476	SM00228	PDZ/DHR/GLGF
	IPR000159	615	701	SM00314	Ras-association
	IPR001895	722	959	SM00147	Guanine-nucleotide dissociation stimulator CDC25
ProfileScan	IPR000595	144	244	PS50042	Cyclic nucleotide-binding
	IPR000651	276	389	PS50212	Guanine nucleotide exchange factor for Ras-like GTPases
	IPR001478	394	464	PS50106	PDZ/DHR/GLGF
	IPR000159	615	701	PS50200	Ras-association
	IPR001895	726	953	PS50009	Guanine-nucleotide dissociation stimulator CDC25

RT-PCR

Experimental conditions

Chromosome No. 4
Experimental conditions

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