Gene/Protein Characteristic Table for KIAA0033
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Product ID ORK00377
Accession No D26067
Description transmembrane protein 41B, transcript variant 1
Clone name ha02009
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (3269 bp)
Predicted protein sequence (335 aa)
Flexi ORF Clone FXC00377
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0033 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 3269 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq No warning No warning
Integrity of 3' end
Length of 3'UTR 2260 bp
Genome contig ID gi51511727r_9159292
PolyA signal sequence
(AATAAA,-30)
+----*----+----*----+----*----+----
TTAAAAATAAAAGAGCTTAGACTCAGTAGGAACTC
Flanking genome sequence
(99995 - 99946)
----+----*----+----*----+----*----+----*----+----*
AGTAGAAGCTTCACTATTTACTCCAGCGTGTGTAAATTGTACTTACTCTA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 r 9259287 9292697 7 99.3 Perfect prediction
Features of the protein sequence
Description

Length: 335 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q5BJD5 1.9e-114 100.0 Transmembrane p...
Homo sapiens
BAG64402 7.3e-114 99.7 unnamed protein...
Homo sapiens
Q5RBZ8 3.3e-111 97.6 Transmembrane p...
Pongo abelii
XP_001504961 8.5e-107 94.2 similar to MGC1...
Equus caballus
XP_851421 5.1e-106 93.8 similar to tran...
Canis lupus fam...
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR015414 173 294 PF09335 SNARE associated Golgi protein
ScanRegExp IPR002114 302 317 PS00589 Phosphotransferase system

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 1 VVSGEEVAPEVALAVAL 17 SECONDARY 17
2 96 MSLLILVSIFLSAAFVMFLVYKN 118 PRIMARY 23
3 157 VLVAYFATYIFLQTFAIPGSIFL 179 PRIMARY 23
4 191 LALFLVCLCSGLGASFCYMLSYL 213 PRIMARY 23
5 270 LKVFFIGTFLGVAPPSFVAIKAG 292 SECONDARY 23
6 306 SWNSIFILMILAVLSILPAIFQ 327 PRIMARY 22
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name Stanford G3
Primer_f TAATCATCCTCAAAGCCTGTT
Primer_r TAAATAATCCTCACGCAAAAG
PCR product length 155 bp
PCR conditions 95 °C15 sec60 °C60 sec30 cycles
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