KIAA1698

Product ID	ORK05523
Accession No	AB051485
Description	integrator complex subunit 5
Clone name	fj15036
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (2890 bp) Predicted protein sequence (908 aa)
Source	Human fetal brain
Rouge ID	mKIAA1698 by Kazusa Mouse cDNA Project

Length: 2890 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	163 bp
Genome contig ID	gi51511727r_62070905
PolyA signal sequence (AATAAA,-19)	+--------+--------+----*----+---- CACTTTTTTTTTTCTAAATAAAATTTGCCAACTTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGAAAACCAGTCCAGTGCTTCTGTCATGCTGAGAGCGTGCAAAGTCTGAC

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	11	r	62170905	62173794	1	100.0	Perfect prediction

Length: 908 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q6P9B9	100.0	Integrator comp...	Homo sapiens
XP_001154137	99.8	integrator comp...	Pan troglodytes
XP_001116423	99.3	similar to inte...	Macaca mulatta
XP_001494846	97.9	integrator comp...	Equus caballus
ABM06097	96.7	integrator comp...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	751	KLVAAAPPALCYCSVLLRGLLAA	773	PRIMARY	23
SOSUI2	2	821	SQLAPFEVRLLLLSVWGFLREHG	843	SECONDARY	23

Primer_f	CATCGAGGCAACACAGAACTG
Primer_r	GAAAACTGACCAAATCCCTCC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	unigene
Primer_f	-
Primer_r	-
PCR product length	-
PCR conditions	-