KIAA1581

Product ID	ORK00869
Accession No	AB046801
Description	ANKH inorganic pyrophosphate transport regulator
Clone name	fj05690
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (3928 bp) Predicted protein sequence (545 aa)
HaloTag ORF Clone	FHC00869
Flexi ORF Clone	FXC00869
Source	Human fetal brain
Rouge ID	mKIAA1581 by Kazusa Mouse cDNA Project

Length: 3928 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	5	r	14762019	14924725	12	99.1	Perfect prediction

Length: 545 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9HCJ1	4.3e-175	100.0	Progressive ank...	Homo sapiens
AAF88039	9.5e-175	99.8	progressive ank...	Homo sapiens
Q9JHZ2	2.2e-173	98.4	Progressive ank...	Mus musculus
BAD96351	7.2e-173	99.8	ankylosis, prog...	Homo sapiens
P58366	1.4e-172	97.8	Progressive ank...	Rattus norvegicus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR009887	54	545	PF07260	Progressive ankylosis

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	139	AVLCMVVAGAIAAVFHTLIAYSD	161	PRIMARY	23
	2	184	AFLYLAAFPFMDAMAWTHAGI	204	SECONDARY	21
	3	213	LVGCASISDVIAQVVFVAILLHS	235	PRIMARY	23
	4	244	LIPILSLYMGALVRCTTLCLGYY	266	SECONDARY	23
	5	380	FTFVCMALSLTLCFVMFWTPNVS	402	PRIMARY	23
	6	409	IIGVDFAFAELCVVPLRIFSFFP	431	PRIMARY	23
	7	451	FVLAPSSVLRIIVLIASLVVLPY	473	PRIMARY	23
	8	481	LGVGSLLAGFVGESTMVAIAAC	502	SECONDARY	22

Primer_f	GCCCACATCAAGAAGTTCACC
Primer_r	TGACTGGAACTGGGAAGAAGG
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	RH-map
Primer_f	ACAGGTTAAAACTCGGCTTCC
Primer_r	GAAATTTAAGAGGCCACCGGG
PCR product length	86 bp
PCR conditions	95 °C15 sec64 °C60 sec30 cycles