Gene/Protein Characteristic Table for KIAA1154
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Product ID ORK00190
Accession No AB032980
Description doublecortin domain containing 2, transcript variant 1
Clone name hh03679s1
Vector information
The cDNA fragment was originally inserted at SalI-NotI site ...
cDNA sequence DNA sequence (6548 bp)
Predicted protein sequence (476 aa)
Flexi ORF Clone FXC00190
Source Human adult brain
Rouge ID mKIAA1154 by Kazusa Mouse cDNA Project
Note We replaced hh03679, former representative clones for KIAA1154 with hh03679s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 6548 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 5117 bp
Genome contig ID gi89161210r_24178103
PolyA signal sequence
(None)
+----*----+----*----+----*----+----
ACTCCAGCCTGGGCGACAGAGCGAGACTCCGTCTC
Flanking genome sequence
(99719 - 99670)
----+----*----+----*----+----*----+----*----+----*
AAAAAAAAAAAAAAAAAGAATGTCTCTTTAAGGAAAATTATTTGTATTTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 6 r 24277822 24465957 10 99.2 Perfect prediction
Features of the protein sequence
Description

Length: 476 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9UHG0 2e-166 99.8 Doublecortin do...
Homo sapiens
AAH50704 2.3e-166 99.8 DCDC2 protein [...
Homo sapiens
XP_001100219 3.7e-160 95.2 doublecortin do...
Macaca mulatta
XP_001495221 2.3e-150 89.3 similar to RU2S...
Equus caballus
XP_001928023 3.6e-148 88.7 similar to RU2S...
Sus scrofa
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
Entry Exp ID% HUGE_ID
AB002367 3.1e-10 30.6 KIAA0369
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR003533 34 95 PF03607 Doublecortin
IPR003533 156 216 PF03607 Doublecortin
HMMSmart IPR003533 12 100 SM00537 Doublecortin
IPR003533 134 221 SM00537 Doublecortin
ProfileScan IPR003533 17 100 PS50309 Doublecortin
IPR003533 139 221 PS50309 Doublecortin
Expression profile
Description

RT-PCR-ELISA
Experimental conditions
Primer_f TTAGGTTGCCACACTCATAGG
Primer_r ACTCTGAACCACGTATGTATG
PCR conditions 95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

RH mapping information
Description

Chromosome No. 6
Experimental conditions
Panel name unigene
Primer_f -
Primer_r -
PCR product length -
PCR conditions -
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