KIAA0950

Product ID	ORK02007
Accession No	AB023167
Description	Fas apoptotic inhibitory molecule 2
Clone name	hj05289
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (4640 bp) Predicted protein sequence (343 aa)
HaloTag ORF Clone	FHC02007
Flexi ORF Clone	FXC02007
Source	Human adult brain
Rouge ID	mKIAA0950 by Kazusa Mouse cDNA Project

Length: 4640 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	3607 bp
Genome contig ID	gi89161190r_48446947
PolyA signal sequence (AATAAA,-23)	+--------+--------+----*----+---- ACTGGAGATAAAAATAAAAGCAATTGTGACACCTG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTGTTTCCCTCGTCTGCCGTGTGCTGGTTGCCTCAGATGGTCGTGGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	r	48546947	48583924	12	99.2	Perfect prediction

Length: 343 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q9BWQ8	1.2e-109	100.0	Fas apoptotic i...	Homo sapiens
Q5R4I4	3.8e-109	99.7	Fas apoptotic i...	Pongo abelii
XP_001504277	8.3e-107	96.2	similar to Fas ...	Equus caballus
XP_001110495	5.7e-106	97.2	similar to Fas ...	Macaca mulatta
Q1LZ71	2.1e-105	94.9	Fas apoptotic i...	Bos taurus

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006214	122	342	PF01027	Protein of unknown function UPF0005

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	133	VYTILLIQLLVTLAVVALFTFCD	155	PRIMARY	23
	2	169	WASYAVFFATYLTLACCSG	187	SECONDARY	19
	3	192	FPWNLILLTVFTLSMAYLTGMLS	214	SECONDARY	23
	4	221	SVLLCLGITALVCLSVTVFS	240	PRIMARY	20
	5	252	GVLFVLLMTLFFSGLILAILLPF	274	PRIMARY	23
	6	282	AVYAALGAGVFTLFLALDTQLLM	304	PRIMARY	23
	7	316	YIFGALNIYLDIIYIFTFFLQLF	338	SECONDARY	23

Primer_f	AATCTGACTGTCCGGAATGTG
Primer_r	AAGAGTAGGGAATGGAAGAAC
PCR conditions	95 °C30 sec55 °C30 sec72 °C60 sec30 cycles

Panel name	GeneBridge 4
Primer_f	AATCTGACTGTCCGGAATGTG
Primer_r	AAGAGTAGGGAATGGAAGAAC
PCR product length	178 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles