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Gene/Protein Characteristic Table for KIAA0676

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Product ID	ORK07069
Accession No	AB014576
Description	TBC1 domain family, member 9B (with GRAM domain)
Clone name	hk02596
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector. This cDNA clone hk02596 has an irregular adapter sequence (GTCGACCCAC GCGTCCGCCC ACGCGTCCG) instead of regular one (GTCGACCCAC GCGTCCG).
cDNA sequence	DNA sequence (4491 bp) Predicted protein sequence (1262 aa)
Source	Human adult brain
Rouge ID	mKIAA0676 by Kazusa Mouse cDNA Project

Length: 4491 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

Length: 1262 aa
Result of homology search against nr database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR004182	154	221	PF02893	GRAM
	IPR004182	300	368	PF02893	GRAM
	IPR000195	517	729	PF00566	RabGAP/TBC
	IPR002048	895	923	PF00036	Calcium-binding EF-hand
HMMSmart	IPR004182	154	221	SM00568	GRAM
	IPR004182	300	368	SM00568	GRAM
	IPR000195	517	730	SM00164	RabGAP/TBC
ProfileScan	IPR000195	520	707	PS50086	RabGAP/TBC
	IPR002048	891	926	PS50222	Calcium-binding EF-hand

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	611	VTSVLLLYGSEEEAFWLLVALCE	633	SECONDARY	23
	2	670	QDLGVISSISLSWFLTLFLSVMP	692	PRIMARY	23
	3	698	VIVDCFFYEGIKVILQVALAVLD	720	PRIMARY	23

RT-PCR

RT-PCR-ELISA

Experimental conditions

Chromosome No. 5
Experimental conditions

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