Gene/Protein Characteristic Table for KIAA0238
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Product ID ORK00473
Accession No D87075
Description solute carrier family 23 (ascorbic acid transporter), member 2, transcript variant 1
Clone name fg04858
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (6941 bp)
Predicted protein sequence (676 aa)
Flexi ORF Clone FXC00473
Source Human fetal brain
Rouge ID mKIAA0238 by Kazusa Mouse cDNA Project
Note We replaced ha02544, former representative clones for KIAA0238 with fg04858. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 6941 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning Warning
Integrity of 3' end
Length of 3'UTR 4613 bp
Genome contig ID gi51511747r_4681005
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
AACTGTTCCGTACAAATAAAAGCTCTCCTGGACAC
Flanking genome sequence
(100000 - 99951)
----+----*----+----*----+----*----+----*----+----*
AGTTTTGCTCTTCACGTGCCTCCTTCTTGGGCTACTCTTTAAGAAAAGAG

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 20 r 4781005 4930145 17 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 676 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9UGH3 0 100.0 Solute carrier ...
Homo sapiens
EAX10438 0 99.8 solute carrier ...
Homo sapiens
ABK34450 0 99.8 sodium-ascorbic...
Homo sapiens
AAC78806 0 99.8 yolk sac permea...
Homo sapiens
XP_001164748 0 100.0 solute carrier ...
Pan troglodytes
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR006043 126 562 PF00860 Xanthine/uracil/vitamin C permease
ScanRegExp IPR006042 508 528 PS01116 Xanthine/uracil permease

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 140 FSGTIAVPFLLADAMCVGYDQWA 162 SECONDARY 23
2 164 SQLIGTIFFCVGITTLLQTTFGC 186 SECONDARY 23
3 240 EIQGAIIMSSLIEVVIGLLGLPG 262 PRIMARY 23
4 267 YIGPLTITPTVALIGLSGFQAAG 289 SECONDARY 23
5 292 AGKHWGIAMLTIFLVLLFSQYAR 314 PRIMARY 23
6 336 LFKMFPIILAILVSWLLCFIFTV 358 PRIMARY 23
7 398 LPTVSAAGVIGMLSAVVASIIES 420 PRIMARY 23
8 484 VIQCGAALMLALGMIGKFSALFA 506 PRIMARY 23
9 512 VLGALFCTLFGMITAVGLSNLQF 534 SECONDARY 23
10 540 SRNLFVLGFSIFFGLVLPSYLRQ 562 SECONDARY 23
11 571 GIDQVLNVLLTTAMFVGGCVAFI 593 SECONDARY 23
Expression profile
Description

Northern blot
RT-PCR-ELISA
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name Genebridge 4
Primer_f ACGTTGCCATCTTACTGACAG
Primer_r AACAACCCCACCCCAAATACC
PCR product length 223 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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