KIAA0238

Product ID	ORK00473
Accession No	D87075
Description	solute carrier family 23 (ascorbic acid transporter), member 2, transcript variant 1
Clone name	fg04858
Vector information	The cDNA fragment was inserted at the SalI-NotI site of the ... The cDNA fragment was inserted at the SalI-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the SalI adapter (5'TCGACCCACG CGTCCG3') and inserted at the SalI site of the vector. The 3'end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6941 bp) Predicted protein sequence (676 aa)
HaloTag ORF Clone	FHC00473
Flexi ORF Clone	FXC00473
Source	Human fetal brain
Rouge ID	mKIAA0238 by Kazusa Mouse cDNA Project
Note	We replaced ha02544, former representative clones for KIAA0238 with fg04858. (2002/5/10)

Length: 6941 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	Warning

Integrity of 3' end

Length of 3'UTR	4613 bp
Genome contig ID	gi51511747r_4681005
PolyA signal sequence (AATAAA,-21)	+--------+--------+----*----+---- AACTGTTCCGTACAAATAAAAGCTCTCCTGGACAC
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* AGTTTTGCTCTTCACGTGCCTCCTTCTTGGGCTACTCTTTAAGAAAAGAG

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	20	r	4781005	4930145	17	100.0	Perfect prediction

Length: 676 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q9UGH3	100.0	Solute carrier ...	Homo sapiens
EAX10438	99.8	solute carrier ...	Homo sapiens
ABK34450	99.8	sodium-ascorbic...	Homo sapiens
AAC78806	99.8	yolk sac permea...	Homo sapiens
XP_001164748	100.0	solute carrier ...	Pan troglodytes

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

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Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
HMMPfam	IPR006043	126	562	PF00860	Xanthine/uracil/vitamin C permease
ScanRegExp	IPR006042	508	528	PS01116	Xanthine/uracil permease

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	140	FSGTIAVPFLLADAMCVGYDQWA	162	SECONDARY	23
	2	164	SQLIGTIFFCVGITTLLQTTFGC	186	SECONDARY	23
	3	240	EIQGAIIMSSLIEVVIGLLGLPG	262	PRIMARY	23
	4	267	YIGPLTITPTVALIGLSGFQAAG	289	SECONDARY	23
	5	292	AGKHWGIAMLTIFLVLLFSQYAR	314	PRIMARY	23
	6	336	LFKMFPIILAILVSWLLCFIFTV	358	PRIMARY	23
	7	398	LPTVSAAGVIGMLSAVVASIIES	420	PRIMARY	23
	8	484	VIQCGAALMLALGMIGKFSALFA	506	PRIMARY	23
	9	512	VLGALFCTLFGMITAVGLSNLQF	534	SECONDARY	23
	10	540	SRNLFVLGFSIFFGLVLPSYLRQ	562	SECONDARY	23
	11	571	GIDQVLNVLLTTAMFVGGCVAFI	593	SECONDARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	ACGTTGCCATCTTACTGACAG
Primer_r	AACAACCCCACCCCAAATACC
PCR product length	223 bp
PCR conditions	95 °C15 sec62 °C60 sec30 cycles