KIAA0233

Product ID	ORK04967
Accession No	D87071
Description	piezo-type mechanosensitive ion channel component 1
Clone name	ha04602
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6368 bp) Predicted protein sequence (2035 aa)
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0233 by Kazusa Mouse cDNA Project

Length: 6368 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	Warning	No warning

Integrity of 3' end

Length of 3'UTR	258 bp
Genome contig ID	gi51511732r_87209256
PolyA signal sequence (ATTAAA,-29)	+--------+--------+----*----+---- TTTTTAATTAAAAAATGTTTTATTTATACAAATGG
Flanking genome sequence (100000 - 99951)	----+--------+--------+--------+--------+----* ACAATCAGAGGCCAGTCCCCCGTCCTTGCCTTCCGGCTCCAGTGTGGTGT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	16	r	87309256	87330160	40	99.7	Perfect prediction

Length: 2035 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	ID%	Protein	Source
Q92508	100.0	Protein FAM38A;...	Homo sapiens
BAF03565	100.0	Mib [Homo sapiens].	Homo sapiens
BAG10304	100.0	FAM38A protein ...	synthetic construct
EAW66773	100.0	hCG1980844, iso...	Homo sapiens
EAW66770	99.9	hCG1980844, iso...	Homo sapiens

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
ScanRegExp	IPR003006	1194	1200	PS00290	Immunoglobulin/major histocompatibility complex motif

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	27	PCLDLGAMLLYTLTFWLLLRQFV	49	SECONDARY	23
	2	106	VSFAGRLVVYKIVYMFLFLLCLT	128	PRIMARY	23
	3	141	LKAFWWLVVAYTMLVLIAVYTF	162	PRIMARY	22
	4	194	ELFSSILVPGFFLLACILQLHYF	216	PRIMARY	23
	5	335	YTVWVALKEVSVMNLLLVVLWAF	357	PRIMARY	23
	6	368	ASCLSTVWTCVIIVCKMLYQLKV	390	SECONDARY	23
	7	495	GCLKYFINFFFYKFGLEICFLMA	517	SECONDARY	23
	8	520	VIGQRMNFLVTLHGCWLVAILT	541	SECONDARY	22
	9	557	LFLALFLLYQYLLCLGMPPALCI	579	PRIMARY	23
	10	670	DMLKVAVFRYLFWLVLVVVFVTG	692	PRIMARY	23
	11	697	SIFGLGYLLACFYLLLFGTALLQ	719	PRIMARY	23
	12	727	VLWDCLILYNVTVIISKNMLSLL	749	PRIMARY	23
	13	754	VEQMQTGFCWVIQLFSLVCTVK	775	SECONDARY	22
	14	792	LPVEEAGIIWDSVCFFFLLLQR	813	SECONDARY	22
	15	1188	RLLRAVYQCVAAHSELLCYFIII	1210	SECONDARY	23
	16	1217	ASAGSLVLPVLVFLWAMLSIPRP	1239	PRIMARY	23
	17	1243	FWMTAIVFTEIAVVVKYLFQFGF	1265	SECONDARY	23
	18	1473	DVYALMFLADVVDFIIIIFGFWA	1495	PRIMARY	23
	19	1515	PEAFLVMLLIQFSTMVVDRALYL	1537	SECONDARY	23
	20	1545	LAFQVALVLAIHLWMFFILPAVT	1567	PRIMARY	23
	21	1573	QNVVAQLWYFVKCIYFALSAYQI	1595	SECONDARY	23
	22	1692	LIILFLIAIIWFPLLFMSLVRSV	1714	PRIMARY	23
	23	1954	IMGLYVSIVLVIGKFVRGFFSEI	1976	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	CTGGTCATCGGCAAGTTCGTG
Primer_r	GGTGAGCGGTAGAGGAAGATG
PCR product length	185 bp
PCR conditions	95 °C15 sec66 °C60 sec30 cycles