Gene/Protein Characteristic Table for KIAA0180
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Product ID ORK00446
Accession No D80002
Description protein O-fucosyltransferase 1, transcript variant 1
Clone name ha02567s1
Vector information
The cDNA fragment was inserted at the SalI-NotI site of the ...
cDNA sequence DNA sequence (5189 bp)
Predicted protein sequence (403 aa)
Flexi ORF Clone FXC00446
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0180 by Kazusa Mouse cDNA Project
Note We replaced ha02567, former representative clones for KIAA0180 with ha02567s1. (2002/5/10)
Features of the cloned cDNA sequence
Description

Length: 5189 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 3976 bp
Genome contig ID gi51511747f_30159360
PolyA signal sequence
(AATAAA,-9)
+----*----+----*----+----*----+----
TCAAATATAAATAAAAAATTGTGAGTAATAAAATG
Flanking genome sequence
(130743 - 130792)
----+----*----+----*----+----*----+----*----+----*
AACTCACAGTTTCAACAATGACCCACATTTTACCAGTCTAGTTGCATTGA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 20 f 30259360 30290101 7 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 403 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q9H488 3e-174 100.0 GDP-fucose prot...
Homo sapiens
BAE88386 3.2e-171 97.9 unnamed protein...
Macaca fascicularis
CAH91412 3.7e-171 98.5 hypothetical pr...
Pongo abelii
AAQ02332 6.2e-164 92.1 protein O-fucos...
Bos taurus
CAH03714 1.2e-162 91.0 protein-O-fucos...
Bos taurus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
None - - - - -

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 19 AAWARPLSVSFLLLLLPLPGMP 40 PRIMARY 22
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 20
Experimental conditions
Panel name Genebridge 4
Primer_f AAGGAGGTGGGAAATGATTAG
Primer_r AACAGGGAAAACATGATACAC
PCR product length 150 bp
PCR conditions 95 °C15 sec64 °C60 sec30 cycles
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