KIAA0152

Product ID	ORK00431
Accession No	D63486
Description	malectin, transcript variant 1
Clone name	ha03561
Vector information	The cDNA fragment was inserted at the EcoRV-NotI site of the ... The cDNA fragment was inserted at the EcoRV-NotI site of the pBluescript II SK(+) vector (GenBank Accession No. X52328, AmpR). The 5' end of the cDNA was repaired and ligated with the EcoRV (blunt-end ligation) site without using any linker or adapter. The 3' end of the cDNA was inserted at the NotI site of the vector.
cDNA sequence	DNA sequence (6322 bp) Predicted protein sequence (315 aa)
HaloTag ORF Clone	FHC00431
Flexi ORF Clone	FXC00431
Source	Myeloblast cell line (KG-1)
Rouge ID	mKIAA0152 by Kazusa Mouse cDNA Project

Length: 6322 bp

Physical map

Restriction map

Prediction of protein coding region (GeneMark analysis).

	N-terminal truncation	Coding interruption
cloned DNA seq	No warning	No warning

Integrity of 3' end

Length of 3'UTR	5315 bp
Genome contig ID	gi89161190f_119509355
PolyA signal sequence (AATAAA,-20)	+--------+--------+----*----+---- ATTGCTTTGTGTTTTAATAAACAATTTGCAGTGAC
Flanking genome sequence (114691 - 114740)	----+--------+--------+--------+--------+----* ACTCTGTGTTGGGACTGAGTTCGAACTTGATCTCAAAGCGCTGAAGTGAT

Ensembl ContigView (Add our DAS server as a DAS source)

	Chr	f/r	start	end	exon	identity	class
Ensembl gnome browser	12	f	119609355	119624044	5	99.1	Perfect prediction

Length: 315 aa

Result of homology search against nr database (FASTA output, Multiple alignment)

Entry	Exp	ID%	Protein	Source
Q6ZQI3	1.1e-102	93.5	Malectin; Flags...	Mus musculus
BAC27434	3.9e-102	93.2	unnamed protein...	Mus musculus
AAI46165	3.2e-97	94.5	LOC515309 prote...	Bos taurus
ACH43740	1.3e-88	86.0	putative RIKEN ...	Taeniopygia guttata
Q8AVF4	7e-88	87.0	Malectin-B; Fla...	Xenopus laevis

The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.

Result of homology search against HUGE database (FASTA output, Multiple alignment)

No significant homologues

Result of motif / domain search (InterProScan and SOSUI)

[ zoom out (x2) ]

Result of InterProScan

Search method	interpro_ID	From	To	Entry	Definition
None	-	-	-	-	-

Prediction of transmembrane (TM) segments

Method	No.	N terminal	transmembrane region	C terminal	type	length
SOSUI2	1	20	VVAAMLGAWAVEGTAVALLRLLL	42	PRIMARY	23
	2	55	GVAGVAGAAGAGLPESVIWAVNA	77	SECONDARY	23
	3	291	NSSLMFPILVAFGVFIPTLFCLC	313	PRIMARY	23

Primer_f
Primer_r
PCR conditions	test

Panel name	Genebridge 4
Primer_f	GATAAGAGCAGGTGATTTGGG
Primer_r	GCACTACTGTTTCCTTGGCTG
PCR product length	133 bp
PCR conditions	95 °C15 sec68 °C60 sec30 cycles