Gene/Protein Characteristic Table for KIAA0103
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Product ID ORK00017
Accession No D14659
Description ER membrane protein complex subunit 2
Clone name ha00802
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (1219 bp)
Predicted protein sequence (299 aa)
Flexi ORF Clone FXC00017
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0103 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 1219 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 319 bp
Genome contig ID gi51511724f_109425058
PolyA signal sequence
(AATAAA,-29)
+----*----+----*----+----*----+----
TTGTTAAATAAACCATGATGATTTATTAAACTTGC
Flanking genome sequence
(143266 - 143315)
----+----*----+----*----+----*----+----*----+----*
ATATGAAGATTCTAACTTCATTTTGTTATACTCACAGTGGATATATTGTT

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 8 f 109525058 109568322 11 100.0 Perfect prediction
Features of the protein sequence
Description

Length: 299 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
XP_001090826 7.7e-118 99.7 hypothetical pr...
Macaca mulatta
Q5R882 1.4e-117 99.7 Tetratricopepti...
Pongo abelii
AAH20753 1.6e-117 99.7 Tetratricopepti...
Homo sapiens
BAF83663 1.6e-117 99.7 unnamed protein...
Homo sapiens
XP_001495024 1.3e-116 99.3 similar to Tetr...
Equus caballus
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR013105 89 122 PF07719 Tetratricopeptide TPR_2
IPR013105 157 190 PF07719 Tetratricopeptide TPR_2
HMMSmart IPR013026 89 122 SM00028 Tetratricopeptide region
IPR013026 157 190 SM00028 Tetratricopeptide region
IPR013026 194 227 SM00028 Tetratricopeptide region
ProfileScan IPR013026 89 122 PS50005 Tetratricopeptide region
IPR013026 89 227 PS50293 Tetratricopeptide region
IPR013026 157 190 PS50005 Tetratricopeptide region
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 8
Experimental conditions
Panel name Genebridge 4
Primer_f CATTAGATTTCACAACTGCAC
Primer_r CTCTCTTCCATGGATTTCCCC
PCR product length 184 bp
PCR conditions 95 °C15 sec62 °C60 sec30 cycles
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