Gene/Protein Characteristic Table for KIAA0102
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Product ID ORK00409
Accession No D14658
Description signal peptidase complex subunit 2
Clone name ha00791
Vector information
The cDNA fragment was inserted at the EcoRV-NotI site of the ...
cDNA sequence DNA sequence (1370 bp)
Predicted protein sequence (225 aa)
Flexi ORF Clone FXC00409
Source Myeloblast cell line (KG-1)
Rouge ID mKIAA0102 by Kazusa Mouse cDNA Project
Features of the cloned cDNA sequence
Description

Length: 1370 bp
Physical map
Restriction map
Prediction of protein coding region (GeneMark analysis).

N-terminal truncation Coding interruption
cloned DNA seq Warning No warning
Integrity of 3' end
Length of 3'UTR 691 bp
Genome contig ID gi51511727f_74237981
PolyA signal sequence
(AATAAA,-21)
+----*----+----*----+----*----+----
CTAAAATTTTTTTCAATAAAAGGAAGGAAGATGTG
Flanking genome sequence
(128448 - 128497)
----+----*----+----*----+----*----+----*----+----*
AAAAAAATGGAGTCATAGTTTTGATGGAGAGAACAGAGAAACAAGTGTTA

Ensembl ContigView (Add our DAS server as a DAS source)


Chr f/r start end exon identity class
Ensembl gnome browser 11 f 74337981 74366427 5 100.0 Perfect prediction
Ensembl gnome browser 1 r 28294148 28295518 1 99.0 Perfect prediction
Features of the protein sequence
Description

Length: 225 aa
Result of homology search against nr database (FASTA output, Multiple alignment)
Entry Exp ID% Protein Source
Q15005 7.7e-90 100.0 Signal peptidas...
Homo sapiens
BAG56680 1.4e-89 99.6 unnamed protein...
Homo sapiens
AAH64957 1.6e-89 99.6 SPCS2 protein [...
Homo sapiens
XP_001174940 1.9e-89 99.6 hypothetical pr...
Pan troglodytes
EAX07712 1.8e-88 99.1 hCG18930 [Homo ...
Homo sapiens
The numbers on the left and right sides of a black line in the graphical overview indicate the lengths (in amino acid residues) of the non-homologous N-terminal and C-terminal portions flanking the homologous region (indicated by the black line), respectively.
Result of homology search against HUGE database (FASTA output, Multiple alignment)
No significant homologues
Result of motif / domain search (InterProScan and SOSUI)

Result of InterProScan

Search method interpro_ID From To Entry Definition
HMMPfam IPR009582 42 223 PF06703 Microsomal signal peptidase 25 kDa subunit

Prediction of transmembrane (TM) segments
Method No. N terminal transmembrane region C terminal type length
SOSUI2 1 82 GRLTICTISCFFAIVALIWDYMH 104 PRIMARY 23
2 112 VLALCVISYFVMMGILTIYTSY 133 SECONDARY 22
Expression profile
Description

Northern blot
Experimental conditions
Primer_f
Primer_r
PCR conditions test

RH mapping information
Description

Chromosome No. 11
Experimental conditions
Panel name Genebridge 4
Primer_f AGTGGGAGAACAAAGCAGCAG
Primer_r TTTTTATCTCCCCCACCCCCC
PCR product length 223 bp
PCR conditions 95 °C15 sec70 °C60 sec32 cycles
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